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The map label for this gene is nth [H]

Identifier: 37521390

GI number: 37521390

Start: 1940666

End: 1941328

Strand: Reverse

Name: nth [H]

Synonym: gll1821

Alternate gene names: 37521390

Gene position: 1941328-1940666 (Counterclockwise)

Preceding gene: 37521393

Following gene: 37521388

Centisome position: 41.67

GC content: 64.25

Gene sequence:

>663_bases
ATGGCAATCGAGACAATGGTGACTCCCGGGGGGGTGCGGGCGGTGATGGAGGGTCTGGCGGCCACCTACCGCGGCCGGGG
CTCGGTGGAATTGGGCGAGCCTTACCGGGTGTTGGTCTCGACGGTGATTTCCCAGCGCACCCGCGAAGAGCAGACCACAG
CGGTCTCGCAGCGCGTCTTCGCGCGCTATCCGGACATGGCTTCACTGGCTGCGGCCGATGAAAAAGAATTGCTCGTGCTG
CTTGCCGGCAGCGAGTACCGGGAGGCCAAAGGGCCGCGCCTGATTGCCATGGCGACAATCCTGCTTGAAAAGTACGGCGG
CCGGGTTCCTGACGATATCGACGCACTGCTTGCTCTACCGGGCATTGGTCGCAAGACGGCCAACTGTGTGCTCATCTATG
CCTTCAACCGCGAGGCCATCTGCGTCGATACCCACATGCACAAAATCGCCAATCGACTGGGCTGGGTGACAACGAAGACC
CCCGAGCAGACCGAAAAAGCGCTCGAAGTCGTGATGCCGCGCGATCTGTGGGCCGGGAGCAACCGACTGTTTTTGCAGCA
CGGCCGGGCAATCTGCCTGAGCGGCGCTCCGCCCCTGTGCAGCCGCTGCCCGGTTCGCCCCTGGTGCGCCTACGGCCAGG
AGCCGACGGCCCGCAAACGCTGA

Upstream 100 bases:

>100_bases
GATGTTAATGCATCTAGCCGCTGTTTCCCTTCCGTCGGCGGAGAGATAGGCACCGGCGGAGCGGACATTTAGTCTGGAGA
GTACCGATGGCGAGAGGGCG

Downstream 100 bases:

>100_bases
AGCCGGCCGATGTCCGTAAGTCGCGGCGAAGACACCGGGCGGCTGCCAGGAGAACCCCGCAGCCGCCCGGTGCATCATCG
CAGGTCGTAAGTTACTTTTT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAIETMVTPGGVRAVMEGLAATYRGRGSVELGEPYRVLVSTVISQRTREEQTTAVSQRVFARYPDMASLAAADEKELLVL
LAGSEYREAKGPRLIAMATILLEKYGGRVPDDIDALLALPGIGRKTANCVLIYAFNREAICVDTHMHKIANRLGWVTTKT
PEQTEKALEVVMPRDLWAGSNRLFLQHGRAICLSGAPPLCSRCPVRPWCAYGQEPTARKR

Sequences:

>Translated_220_residues
MAIETMVTPGGVRAVMEGLAATYRGRGSVELGEPYRVLVSTVISQRTREEQTTAVSQRVFARYPDMASLAAADEKELLVL
LAGSEYREAKGPRLIAMATILLEKYGGRVPDDIDALLALPGIGRKTANCVLIYAFNREAICVDTHMHKIANRLGWVTTKT
PEQTEKALEVVMPRDLWAGSNRLFLQHGRAICLSGAPPLCSRCPVRPWCAYGQEPTARKR
>Mature_219_residues
AIETMVTPGGVRAVMEGLAATYRGRGSVELGEPYRVLVSTVISQRTREEQTTAVSQRVFARYPDMASLAAADEKELLVLL
AGSEYREAKGPRLIAMATILLEKYGGRVPDDIDALLALPGIGRKTANCVLIYAFNREAICVDTHMHKIANRLGWVTTKTP
EQTEKALEVVMPRDLWAGSNRLFLQHGRAICLSGAPPLCSRCPVRPWCAYGQEPTARKR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=182, Percent_Identity=35.7142857142857, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1787920, Length=186, Percent_Identity=28.494623655914, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1789331, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=176, Percent_Identity=35.7954545454545, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324530, Length=172, Percent_Identity=27.906976744186, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319304, Length=173, Percent_Identity=28.9017341040462, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=189, Percent_Identity=34.9206349206349, Blast_Score=110, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24198; Mature: 24067

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIETMVTPGGVRAVMEGLAATYRGRGSVELGEPYRVLVSTVISQRTREEQTTAVSQRVF
CCCCEECCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARYPDMASLAAADEKELLVLLAGSEYREAKGPRLIAMATILLEKYGGRVPDDIDALLALP
HHCCCHHHHHCCCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCC
GIGRKTANCVLIYAFNREAICVDTHMHKIANRLGWVTTKTPEQTEKALEVVMPRDLWAGS
CCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHCCCC
NRLFLQHGRAICLSGAPPLCSRCPVRPWCAYGQEPTARKR
CEEEEECCCEEEECCCCCHHHCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
AIETMVTPGGVRAVMEGLAATYRGRGSVELGEPYRVLVSTVISQRTREEQTTAVSQRVF
CCCEECCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARYPDMASLAAADEKELLVLLAGSEYREAKGPRLIAMATILLEKYGGRVPDDIDALLALP
HHCCCHHHHHCCCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCC
GIGRKTANCVLIYAFNREAICVDTHMHKIANRLGWVTTKTPEQTEKALEVVMPRDLWAGS
CCCCCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHCCCC
NRLFLQHGRAICLSGAPPLCSRCPVRPWCAYGQEPTARKR
CEEEEECCCEEEECCCCCHHHCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]