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The map label for this gene is glyS [H]

Identifier: 37521215

GI number: 37521215

Start: 1760302

End: 1762476

Strand: Direct

Name: glyS [H]

Synonym: gvip230

Alternate gene names: 37521215

Gene position: 1760302-1762476 (Clockwise)

Preceding gene: 37521208

Following gene: 37521216

Centisome position: 37.78

GC content: 67.63

Gene sequence:

>2175_bases
ATGGCCACGTTTCTGCTTGAAATCGGCACCGAGGAATTGCCCGCGAGCTTTGTGCCCGGCGCCCTGGCCCAACTGGAGGC
GCGGGTGCGTTCCGCTTTGGCGGAAGCGCAGATCACAGCGGAAACATTGAAGTTATTTGGTACCCCCCGGCGACTGGCGG
TGCTCGCCGAGGGGTTGCCCGAGCGCCAGCCCGACCGCGAAGAAGAACTCAAAGGCCCCCCCGCCCGGGTAGCCTTCGAT
GCCGGGGGCCGCCCCACTGCGCAGGCGGAAGGTTTCGCGCGCAAAGCCGGCGTGGCGGTGGGCGAGCTGAGCGTGCGCGC
CACCGAGAAGGGTGAAGTCGTCTTCGCCGTGCGCCGCATCGAGGGCCGACCGGTGACCCAGCTGCTCGCCGAGCAGATCC
CCGAGTGGATAACCGGCCTCGAAGGGCCGCGGCTGATGCGGTGGGCCGACGGCGACTTGCGCTTCAGCCGCCCGATTCGC
TGGCTGGTGGCCCTGGTGGATAGTGAAGTGCTGCCGGTGGAAATGGCCGGGGTGAGCGCGGGGCGCACCAGCCGCGGCCA
CCGGGTGCTCCATCCCGAACCGATCGAACTGGCCACCGCCGCCGACTACCCGGCGGCCCTCACAGCGGCCTTTGTGCTCG
CCGACCCCGAGGTGCGGCGCGAGCGCATCCGCACCGGCATCGCGGCGGTGGCGGAGCAACTGGGGGCGGAGGCGGCGGTA
CCGGCGGCCCTGCTGGAGGAAGTCAATTTTCTGGTGGAGTGGCCGACGGCGGTGGCGGGGCAGTTCGAGAGCGAATTTTT
GAGCCTGCCCGCTCCGGTGATCAAAACCGAGATGATCACCCACCAGCGCTACTTTCCCCTGCACCAACCGGGCGATCCCG
AAGCCTTGATTCCCCGCTTCATCACCGTGAGCAACGGCGACCCGCGCTACAGCGAACTGATTGCCCGGGGCAACGGCCGG
GTGATCCGCGCCCGCCTCGCCGACGGCCGCTTCTTCTTTGAAGAGGACCGCAAACAGCCTTTGGAGAAGTTCCTCGGTAA
ACTGCAATCGGTCACCTTTCAGGAGAGCCTCGGTTCGGTGGCCGAGCGCGTCGAGCGCCTCGAAGCGATCGCCCTCTGGC
TGTGCGGGCATCTGGGCCTGGGGGGCGAGCAGACCCGGCTGGTGGCGCGCACCGCCCACCTGTGCAAAGCGGACCTGGCC
ACCCAGATGGTCTACGAATTTCCCGAACTGCAGGGACTGGTGGGGCGCGACTACGCCCGCCTCGACGGCGAGGAGCCGCT
GGTCTGTGCAGGCATCGCCGAGCACTACCAGCCGCGCAACGTCGAGGACGACCTGCCTGCGAGCCTGACCGGACAGGTAG
TCGGGATCGCCGATCGCCTGGAGACGCTGGTGGGCATCTTCGGCCTGGGGATGCTCCCGAGCGGCTCCTCCGACCCGTTC
GCCCTGCGCCGCGCCGCCCTCACGGTGGTGCGCATCGGCTGGGACACCGACTTTGAGCTGGATCTCGTGGAAGCCCTCGG
CTTCACCGTTGAACTATTCGTCTCCCGACAACTGCTCAAGCAACCCGCCGATACCCTCCTCGAGCAGCTGTGCGACTGGT
TCGCCCAGAGGCTCTACGGCCTGCTCGTCGAGCGCGACGGCCTCGATTACGATCTGGTGCTCGCCGTGCTGGGAGCCGTG
GATACCGGCTACACCCGCCGCGCCCTGGCCAACCTGCGCGCCCTGAAGTTGCGGCTCGACACGCTGGTGGCCGCCCGCAA
AAGCGGGCTGCTTGCGCAGATTTACGAGACTGCCGTGCGCGTCGCCCGCCTGGCGGGTAAGGCCGGGATTGGCGATCTCA
CCCTCGAACACTTCGATGCGCAGCTGGTGGCCGACCCGGCAGAAGCAGCGCTGCGCGAGGCGGTCCAAAAGTTTGCCGCC
ACCGCCGAGGCCGCCGAGGCCCAAGAGGATTATCCCGCCCTGCTGGGTGCCCTCGGTGAACTCGCCCCCCAGGTCAGCTG
CTTTTTTGATGCCGTGATGGTGATGGTGCCCGACGAAACCTTGCGCACCAACCGCCTGCGGCTGCTCGCGGTCATCGACA
ACAACGCTCGCCGCTGCTTTGATGTCACCCAGGTCGTCATGGCCGGCGGCCAGAGCGATAATGATCCCATTCACCCCAAG
GTGCGCCATGGCTGA

Upstream 100 bases:

>100_bases
CGCGCTCCTTGCCTGGGTTTCATCATAGGCCCGCTTGCCGCACCGGCAAGTGGGCCTGCAAACCCCCTAAGGTACACTGG
GAAGGCTTTCGAGAACAGGC

Downstream 100 bases:

>100_bases
GCAATCCCCGATCCGGGTGCACTTTCTGCCCGATAACCTGAGTGTGACCGCCCAGAGCGGCGAACTGTTGCTCGAATGTG
CCGAGCGCGCCGGCGTGCGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MATFLLEIGTEELPASFVPGALAQLEARVRSALAEAQITAETLKLFGTPRRLAVLAEGLPERQPDREEELKGPPARVAFD
AGGRPTAQAEGFARKAGVAVGELSVRATEKGEVVFAVRRIEGRPVTQLLAEQIPEWITGLEGPRLMRWADGDLRFSRPIR
WLVALVDSEVLPVEMAGVSAGRTSRGHRVLHPEPIELATAADYPAALTAAFVLADPEVRRERIRTGIAAVAEQLGAEAAV
PAALLEEVNFLVEWPTAVAGQFESEFLSLPAPVIKTEMITHQRYFPLHQPGDPEALIPRFITVSNGDPRYSELIARGNGR
VIRARLADGRFFFEEDRKQPLEKFLGKLQSVTFQESLGSVAERVERLEAIALWLCGHLGLGGEQTRLVARTAHLCKADLA
TQMVYEFPELQGLVGRDYARLDGEEPLVCAGIAEHYQPRNVEDDLPASLTGQVVGIADRLETLVGIFGLGMLPSGSSDPF
ALRRAALTVVRIGWDTDFELDLVEALGFTVELFVSRQLLKQPADTLLEQLCDWFAQRLYGLLVERDGLDYDLVLAVLGAV
DTGYTRRALANLRALKLRLDTLVAARKSGLLAQIYETAVRVARLAGKAGIGDLTLEHFDAQLVADPAEAALREAVQKFAA
TAEAAEAQEDYPALLGALGELAPQVSCFFDAVMVMVPDETLRTNRLRLLAVIDNNARRCFDVTQVVMAGGQSDNDPIHPK
VRHG

Sequences:

>Translated_724_residues
MATFLLEIGTEELPASFVPGALAQLEARVRSALAEAQITAETLKLFGTPRRLAVLAEGLPERQPDREEELKGPPARVAFD
AGGRPTAQAEGFARKAGVAVGELSVRATEKGEVVFAVRRIEGRPVTQLLAEQIPEWITGLEGPRLMRWADGDLRFSRPIR
WLVALVDSEVLPVEMAGVSAGRTSRGHRVLHPEPIELATAADYPAALTAAFVLADPEVRRERIRTGIAAVAEQLGAEAAV
PAALLEEVNFLVEWPTAVAGQFESEFLSLPAPVIKTEMITHQRYFPLHQPGDPEALIPRFITVSNGDPRYSELIARGNGR
VIRARLADGRFFFEEDRKQPLEKFLGKLQSVTFQESLGSVAERVERLEAIALWLCGHLGLGGEQTRLVARTAHLCKADLA
TQMVYEFPELQGLVGRDYARLDGEEPLVCAGIAEHYQPRNVEDDLPASLTGQVVGIADRLETLVGIFGLGMLPSGSSDPF
ALRRAALTVVRIGWDTDFELDLVEALGFTVELFVSRQLLKQPADTLLEQLCDWFAQRLYGLLVERDGLDYDLVLAVLGAV
DTGYTRRALANLRALKLRLDTLVAARKSGLLAQIYETAVRVARLAGKAGIGDLTLEHFDAQLVADPAEAALREAVQKFAA
TAEAAEAQEDYPALLGALGELAPQVSCFFDAVMVMVPDETLRTNRLRLLAVIDNNARRCFDVTQVVMAGGQSDNDPIHPK
VRHG
>Mature_723_residues
ATFLLEIGTEELPASFVPGALAQLEARVRSALAEAQITAETLKLFGTPRRLAVLAEGLPERQPDREEELKGPPARVAFDA
GGRPTAQAEGFARKAGVAVGELSVRATEKGEVVFAVRRIEGRPVTQLLAEQIPEWITGLEGPRLMRWADGDLRFSRPIRW
LVALVDSEVLPVEMAGVSAGRTSRGHRVLHPEPIELATAADYPAALTAAFVLADPEVRRERIRTGIAAVAEQLGAEAAVP
AALLEEVNFLVEWPTAVAGQFESEFLSLPAPVIKTEMITHQRYFPLHQPGDPEALIPRFITVSNGDPRYSELIARGNGRV
IRARLADGRFFFEEDRKQPLEKFLGKLQSVTFQESLGSVAERVERLEAIALWLCGHLGLGGEQTRLVARTAHLCKADLAT
QMVYEFPELQGLVGRDYARLDGEEPLVCAGIAEHYQPRNVEDDLPASLTGQVVGIADRLETLVGIFGLGMLPSGSSDPFA
LRRAALTVVRIGWDTDFELDLVEALGFTVELFVSRQLLKQPADTLLEQLCDWFAQRLYGLLVERDGLDYDLVLAVLGAVD
TGYTRRALANLRALKLRLDTLVAARKSGLLAQIYETAVRVARLAGKAGIGDLTLEHFDAQLVADPAEAALREAVQKFAAT
AEAAEAQEDYPALLGALGELAPQVSCFFDAVMVMVPDETLRTNRLRLLAVIDNNARRCFDVTQVVMAGGQSDNDPIHPKV
RHG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=711, Percent_Identity=34.3178621659634, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 78973; Mature: 78842

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFLLEIGTEELPASFVPGALAQLEARVRSALAEAQITAETLKLFGTPRRLAVLAEGLP
CCEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
ERQPDREEELKGPPARVAFDAGGRPTAQAEGFARKAGVAVGELSVRATEKGEVVFAVRRI
CCCCCCHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEEC
EGRPVTQLLAEQIPEWITGLEGPRLMRWADGDLRFSRPIRWLVALVDSEVLPVEMAGVSA
CCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
GRTSRGHRVLHPEPIELATAADYPAALTAAFVLADPEVRRERIRTGIAAVAEQLGAEAAV
CCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHH
PAALLEEVNFLVEWPTAVAGQFESEFLSLPAPVIKTEMITHQRYFPLHQPGDPEALIPRF
HHHHHHHHHHHHCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCEE
ITVSNGDPRYSELIARGNGRVIRARLADGRFFFEEDRKQPLEKFLGKLQSVTFQESLGSV
EEECCCCCHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERVERLEAIALWLCGHLGLGGEQTRLVARTAHLCKADLATQMVYEFPELQGLVGRDYAR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDGEEPLVCAGIAEHYQPRNVEDDLPASLTGQVVGIADRLETLVGIFGLGMLPSGSSDPF
CCCCCCEEEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ALRRAALTVVRIGWDTDFELDLVEALGFTVELFVSRQLLKQPADTLLEQLCDWFAQRLYG
HHHHHHEEEEEECCCCCCCHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
LLVERDGLDYDLVLAVLGAVDTGYTRRALANLRALKLRLDTLVAARKSGLLAQIYETAVR
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VARLAGKAGIGDLTLEHFDAQLVADPAEAALREAVQKFAATAEAAEAQEDYPALLGALGE
HHHHHCCCCCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPQVSCFFDAVMVMVPDETLRTNRLRLLAVIDNNARRCFDVTQVVMAGGQSDNDPIHPK
HHHHHHHHHHHHHHEECCCHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCC
VRHG
CCCC
>Mature Secondary Structure 
ATFLLEIGTEELPASFVPGALAQLEARVRSALAEAQITAETLKLFGTPRRLAVLAEGLP
CEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
ERQPDREEELKGPPARVAFDAGGRPTAQAEGFARKAGVAVGELSVRATEKGEVVFAVRRI
CCCCCCHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCEEEEEEEC
EGRPVTQLLAEQIPEWITGLEGPRLMRWADGDLRFSRPIRWLVALVDSEVLPVEMAGVSA
CCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCC
GRTSRGHRVLHPEPIELATAADYPAALTAAFVLADPEVRRERIRTGIAAVAEQLGAEAAV
CCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHH
PAALLEEVNFLVEWPTAVAGQFESEFLSLPAPVIKTEMITHQRYFPLHQPGDPEALIPRF
HHHHHHHHHHHHCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHCCEE
ITVSNGDPRYSELIARGNGRVIRARLADGRFFFEEDRKQPLEKFLGKLQSVTFQESLGSV
EEECCCCCHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERVERLEAIALWLCGHLGLGGEQTRLVARTAHLCKADLATQMVYEFPELQGLVGRDYAR
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDGEEPLVCAGIAEHYQPRNVEDDLPASLTGQVVGIADRLETLVGIFGLGMLPSGSSDPF
CCCCCCEEEECHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ALRRAALTVVRIGWDTDFELDLVEALGFTVELFVSRQLLKQPADTLLEQLCDWFAQRLYG
HHHHHHEEEEEECCCCCCCHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
LLVERDGLDYDLVLAVLGAVDTGYTRRALANLRALKLRLDTLVAARKSGLLAQIYETAVR
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VARLAGKAGIGDLTLEHFDAQLVADPAEAALREAVQKFAATAEAAEAQEDYPALLGALGE
HHHHHCCCCCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPQVSCFFDAVMVMVPDETLRTNRLRLLAVIDNNARRCFDVTQVVMAGGQSDNDPIHPK
HHHHHHHHHHHHHHEECCCHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCC
VRHG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]