Click here to switch to the map view.

The map label for this gene is ntcB [H]

Identifier: 37521127

GI number: 37521127

Start: 1678891

End: 1679871

Strand: Direct

Name: ntcB [H]

Synonym: gvip213

Alternate gene names: 37521127

Gene position: 1678891-1679871 (Clockwise)

Preceding gene: 37521124

Following gene: 37521128

Centisome position: 36.04

GC content: 64.42

Gene sequence:

>981_bases
ATGCACGGAGTGACTAACGGTTGGGGTTCCGGGTTGGATTCAAGGCAGCTGCAGTACTTTTTGGCATTGAATCATTGCGG
CAGTTTCAGGCTTGCCGCCGAGCAGTGTGGCGTTTCCCAGCCAGGGCTCAGCAAGCAGATCATGGCTCTCGAAGGCGAGC
TTGGGGGACCGTTGTTCGACCGTACCGGTCGGCGGGCATCCCTCACACCCTTGGGCGAATGCTTTTTGCCTTACGCCCGC
CGCGCCTACAAAGAACTCGAACAGTCCCGTGCCGCCATCGGCGAGTTGTTGAAGCCCGATCGGGGCGAAATTTGCATCGC
CGGGCTGCACTCGGTCAACGCCTATTTGCTGCCGGCGCTACTGGCGGTTTTTCGGCAGAAGCATCCCGGTACGCAGCTGC
GCCTCACCTCGCTGGGTTCTGAGCGCATCATCAAGGTGCTGCTCGACCGGTTGGTGGATGCGGCCATTTTGATGGGGCCG
GTCCATTCGCCGGAACTGGTGAGCACGCCGCTCTACGAAGAAGAGCTGGTTGTGCTGCTCCCGGCGCACCACCCGCTCGC
GCGCCGTCCGAGCGTGCGGCTTGCGGAGGTGGCGATCCTGCCGCAGGTGGTCTTCCGCGACGGGTACGCCATGCGCACCG
CCCTGGTGCAGCACTTTCGCGCCATCGGCGCCACGGTCGATGTGGCTGTCGAACTGAATACCCTCGAAGCGTTCAAAGAA
ATGGTCCGTCAGGAAGTGGGGGTGGCGATCCTGCCGCTGTGCGCCGTGCAGCACCTGGGGACGGATTTGGTCATCGCCCG
CCTCGAGGAGCCCTCGATCACCCGGCGCGTCGAACTGGTCTGCCGCAAGGACAACTACCAGGTGCCCGTGGTGGCGGCCT
TTACGCGCCTGGTGGCCGAGCACCTGCCCACCGCCTTCGCCCGCTGGGTCCACACCGGCCCCGAACCCTGGCTGCGCGAA
GCTTCGTACCTGGCGGGCTGA

Upstream 100 bases:

>100_bases
AATAACCTTTCGAGAGCCAGCGTCCCACGAATGCTTTGAAGGAACTTAACTTTACGTAACCCCAGATACATTCGTGGCCG
TGCGGGCCGGATATGGTCTG

Downstream 100 bases:

>100_bases
TGAGCACGGCCTTTCGCGAATTCGTCAAAAAAATCGGCTCTGGTCGCCTCACCGGCCGCGACCTCACCCGTGAAGAATCC
TGCCGGGCGATGGCGATGCT

Product: nitrogen assimilation transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MHGVTNGWGSGLDSRQLQYFLALNHCGSFRLAAEQCGVSQPGLSKQIMALEGELGGPLFDRTGRRASLTPLGECFLPYAR
RAYKELEQSRAAIGELLKPDRGEICIAGLHSVNAYLLPALLAVFRQKHPGTQLRLTSLGSERIIKVLLDRLVDAAILMGP
VHSPELVSTPLYEEELVVLLPAHHPLARRPSVRLAEVAILPQVVFRDGYAMRTALVQHFRAIGATVDVAVELNTLEAFKE
MVRQEVGVAILPLCAVQHLGTDLVIARLEEPSITRRVELVCRKDNYQVPVVAAFTRLVAEHLPTAFARWVHTGPEPWLRE
ASYLAG

Sequences:

>Translated_326_residues
MHGVTNGWGSGLDSRQLQYFLALNHCGSFRLAAEQCGVSQPGLSKQIMALEGELGGPLFDRTGRRASLTPLGECFLPYAR
RAYKELEQSRAAIGELLKPDRGEICIAGLHSVNAYLLPALLAVFRQKHPGTQLRLTSLGSERIIKVLLDRLVDAAILMGP
VHSPELVSTPLYEEELVVLLPAHHPLARRPSVRLAEVAILPQVVFRDGYAMRTALVQHFRAIGATVDVAVELNTLEAFKE
MVRQEVGVAILPLCAVQHLGTDLVIARLEEPSITRRVELVCRKDNYQVPVVAAFTRLVAEHLPTAFARWVHTGPEPWLRE
ASYLAG
>Mature_326_residues
MHGVTNGWGSGLDSRQLQYFLALNHCGSFRLAAEQCGVSQPGLSKQIMALEGELGGPLFDRTGRRASLTPLGECFLPYAR
RAYKELEQSRAAIGELLKPDRGEICIAGLHSVNAYLLPALLAVFRQKHPGTQLRLTSLGSERIIKVLLDRLVDAAILMGP
VHSPELVSTPLYEEELVVLLPAHHPLARRPSVRLAEVAILPQVVFRDGYAMRTALVQHFRAIGATVDVAVELNTLEAFKE
MVRQEVGVAILPLCAVQHLGTDLVIARLEEPSITRRVELVCRKDNYQVPVVAAFTRLVAEHLPTAFARWVHTGPEPWLRE
ASYLAG

Specific function: Seems to regulate utilization of fixed nitrogen by controlling the expression of a certain gene(s) involved in nitrogen metabolism [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=282, Percent_Identity=35.8156028368794, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788481, Length=237, Percent_Identity=33.7552742616034, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788887, Length=186, Percent_Identity=34.9462365591398, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=29.3877551020408, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=28.8617886178862, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=26.8551236749117, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=27.4305555555556, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=26.1044176706827, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788296, Length=221, Percent_Identity=26.6968325791855, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786401, Length=159, Percent_Identity=33.9622641509434, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082132, Length=241, Percent_Identity=28.2157676348548, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790208, Length=303, Percent_Identity=28.3828382838284, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=26.984126984127, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081904, Length=210, Percent_Identity=30.4761904761905, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787601, Length=292, Percent_Identity=25.6849315068493, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790262, Length=293, Percent_Identity=25.5972696245734, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=271, Percent_Identity=24.3542435424354, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=261, Percent_Identity=25.6704980842912, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35907; Mature: 35907

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGVTNGWGSGLDSRQLQYFLALNHCGSFRLAAEQCGVSQPGLSKQIMALEGELGGPLFD
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHHHHHCCCCCCCHHH
RTGRRASLTPLGECFLPYARRAYKELEQSRAAIGELLKPDRGEICIAGLHSVNAYLLPAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
LAVFRQKHPGTQLRLTSLGSERIIKVLLDRLVDAAILMGPVHSPELVSTPLYEEELVVLL
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEE
PAHHPLARRPSVRLAEVAILPQVVFRDGYAMRTALVQHFRAIGATVDVAVELNTLEAFKE
ECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHH
MVRQEVGVAILPLCAVQHLGTDLVIARLEEPSITRRVELVCRKDNYQVPVVAAFTRLVAE
HHHHHHCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHH
HLPTAFARWVHTGPEPWLREASYLAG
HHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHGVTNGWGSGLDSRQLQYFLALNHCGSFRLAAEQCGVSQPGLSKQIMALEGELGGPLFD
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCHHHHHHCCCCCCCHHH
RTGRRASLTPLGECFLPYARRAYKELEQSRAAIGELLKPDRGEICIAGLHSVNAYLLPAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHH
LAVFRQKHPGTQLRLTSLGSERIIKVLLDRLVDAAILMGPVHSPELVSTPLYEEELVVLL
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEEEE
PAHHPLARRPSVRLAEVAILPQVVFRDGYAMRTALVQHFRAIGATVDVAVELNTLEAFKE
ECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHH
MVRQEVGVAILPLCAVQHLGTDLVIARLEEPSITRRVELVCRKDNYQVPVVAAFTRLVAE
HHHHHHCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHEEECCCCCCHHHHHHHHHHHH
HLPTAFARWVHTGPEPWLREASYLAG
HHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7814317 [H]