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The map label for this gene is tnpA [H]

Identifier: 37519940

GI number: 37519940

Start: 385225

End: 386628

Strand: Reverse

Name: tnpA [H]

Synonym: gll0371

Alternate gene names: 37519940

Gene position: 386628-385225 (Counterclockwise)

Preceding gene: 37519941

Following gene: 37519937

Centisome position: 8.3

GC content: 65.17

Gene sequence:

>1404_bases
ATGCCGACCATCGACCCCTGGACCGAACACGAACTGCAACACCTCGATTTGGGCGATGCCCGTCGCCACACCCGACTCAA
GCAGCTGCTCTCCAGCCTCGCCCGTCAACCCCATGCCTCTCTGCCCCAAGCCTGCGAGGATGCCGCCGCCCTCAAAGCCG
CCTACCGCTTTTTGGACAACCCGCAGTGCCACCCTGCAGATATCCGCCAGGCCCATGCCAGTGCCACCGCCGGCCGCCTG
GCTGCCCTACCGCTGGTGCTGCTGATTCAAGACACCACCGAGTTGAACTTCACTGCCCACCCCCACACCACCGGGCTGGG
CCATCTGTCCAAGCCCGAAAGCCAGGGACTGCTGGTCCACTCGCTTCTGGCTGTGCGGCCCGACGCCGTCGCCCTCGGCT
TGTGCTGGCAGAAAGTCTGGACCCGTGCGGCCCAGGTGGAGCATCTGGCCGCTCGACGCCAAAAGCGTCCCCAACAGCAC
AAAGAATCCCAACGCTGGCTGGACGGTCTGGCGGCTGCCAAAGGCTATGTGCCGCCGGGGGGCGAAGCGGTGCTCATCGG
CGACCGCGAGTCGGACATGTTCGGCTTGTTTGCGGCACCGCGCCCGCCGCAGTTGCATCTCTTGGTGCGCGCCGCGCGCC
AACGTCGGCTGGCCACCCCCAAAACGTTGCTGTTCGAGGTTTTCGCGGGTGCCTCAGCCCAGGGCCACTATCGGTGCGAC
CTGGCGCGCCGTCCCGGGCGGGCGGCGCGCCAGGCGAGGTTGCAGGTGTACTGGCAAGCAGTGCAGTTGCAGCCGGGTCG
CAATGACCAGACGCACAAGGGTCAACCGCCGGTGTCGGTGTGGGTGGTGCGCGCCTGGGAAGTGGACCCGCCTGCGGGCG
AAGAGGGTATCGATTGGTGGTTGTTGAGTAGCCAGCCGGTGACCACGCTGGAGCAGGCGCTGGCCTGTGTGCACCGGTAC
ACGCTGCGCTGGTTAATCGAGCGCTATCACTACATTCTCAAAAGTGGTTGCGCGGTGGAGCAGTTGCAGTTGGAGACGGC
ACAGCGTTTGGAGCGTGCGTTGGCAGTCTATTGCATCGTGGCGTGGCGGTTGTTGCAATTGACTTACCAGTCTCGCAGCG
AGCCGGAGTTGCCGTGCACGGTGGCGTTGGCGCAGCACGAGTGGCAGGCGTTGTATTGTCGGATGACCCAGAGTCGGCAG
TTGCCCGAGGAGGCGCCGAGTTTACGTCAGGCGGTGCGGTGGATAGCGCAGTTGGGAGGCTTTGTCGGTCGTAAAGGGGA
CGGTGAGCCTGGGGTGAAGACGCTGTGGCGAGGCTGGCAGCGGCTGGAGGATTTGGCAGCAGGGTGGCTCATCGCCCGCT
CAGCGATGGCGGGCGATGGGAACGAAGATGTGGGTAACGGATAG

Upstream 100 bases:

>100_bases
GCACCCCCTCGACGCGGCTATCCATTACCCACATTTTTGAGAGCGCCTCTGTCTGCTGTGCGCCAGTCACACTGGGAGCG
TACTGGCTCAGCCGTGGCCC

Downstream 100 bases:

>100_bases
CGACGCGGGGAGGGGGAGAGCGCGAGAGGGGAACCCGAAGGGGGTTCCGCCTCTCGCACCCACAGACTGTCGTGCCAAAC
CCACTACCAGGTGTCAGGGT

Product: putative transposase

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPTIDPWTEHELQHLDLGDARRHTRLKQLLSSLARQPHASLPQACEDAAALKAAYRFLDNPQCHPADIRQAHASATAGRL
AALPLVLLIQDTTELNFTAHPHTTGLGHLSKPESQGLLVHSLLAVRPDAVALGLCWQKVWTRAAQVEHLAARRQKRPQQH
KESQRWLDGLAAAKGYVPPGGEAVLIGDRESDMFGLFAAPRPPQLHLLVRAARQRRLATPKTLLFEVFAGASAQGHYRCD
LARRPGRAARQARLQVYWQAVQLQPGRNDQTHKGQPPVSVWVVRAWEVDPPAGEEGIDWWLLSSQPVTTLEQALACVHRY
TLRWLIERYHYILKSGCAVEQLQLETAQRLERALAVYCIVAWRLLQLTYQSRSEPELPCTVALAQHEWQALYCRMTQSRQ
LPEEAPSLRQAVRWIAQLGGFVGRKGDGEPGVKTLWRGWQRLEDLAAGWLIARSAMAGDGNEDVGNG

Sequences:

>Translated_467_residues
MPTIDPWTEHELQHLDLGDARRHTRLKQLLSSLARQPHASLPQACEDAAALKAAYRFLDNPQCHPADIRQAHASATAGRL
AALPLVLLIQDTTELNFTAHPHTTGLGHLSKPESQGLLVHSLLAVRPDAVALGLCWQKVWTRAAQVEHLAARRQKRPQQH
KESQRWLDGLAAAKGYVPPGGEAVLIGDRESDMFGLFAAPRPPQLHLLVRAARQRRLATPKTLLFEVFAGASAQGHYRCD
LARRPGRAARQARLQVYWQAVQLQPGRNDQTHKGQPPVSVWVVRAWEVDPPAGEEGIDWWLLSSQPVTTLEQALACVHRY
TLRWLIERYHYILKSGCAVEQLQLETAQRLERALAVYCIVAWRLLQLTYQSRSEPELPCTVALAQHEWQALYCRMTQSRQ
LPEEAPSLRQAVRWIAQLGGFVGRKGDGEPGVKTLWRGWQRLEDLAAGWLIARSAMAGDGNEDVGNG
>Mature_466_residues
PTIDPWTEHELQHLDLGDARRHTRLKQLLSSLARQPHASLPQACEDAAALKAAYRFLDNPQCHPADIRQAHASATAGRLA
ALPLVLLIQDTTELNFTAHPHTTGLGHLSKPESQGLLVHSLLAVRPDAVALGLCWQKVWTRAAQVEHLAARRQKRPQQHK
ESQRWLDGLAAAKGYVPPGGEAVLIGDRESDMFGLFAAPRPPQLHLLVRAARQRRLATPKTLLFEVFAGASAQGHYRCDL
ARRPGRAARQARLQVYWQAVQLQPGRNDQTHKGQPPVSVWVVRAWEVDPPAGEEGIDWWLLSSQPVTTLEQALACVHRYT
LRWLIERYHYILKSGCAVEQLQLETAQRLERALAVYCIVAWRLLQLTYQSRSEPELPCTVALAQHEWQALYCRMTQSRQL
PEEAPSLRQAVRWIAQLGGFVGRKGDGEPGVKTLWRGWQRLEDLAAGWLIARSAMAGDGNEDVGNG

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 52242; Mature: 52111

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTIDPWTEHELQHLDLGDARRHTRLKQLLSSLARQPHASLPQACEDAAALKAAYRFLDN
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
PQCHPADIRQAHASATAGRLAALPLVLLIQDTTELNFTAHPHTTGLGHLSKPESQGLLVH
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHH
SLLAVRPDAVALGLCWQKVWTRAAQVEHLAARRQKRPQQHKESQRWLDGLAAAKGYVPPG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
GEAVLIGDRESDMFGLFAAPRPPQLHLLVRAARQRRLATPKTLLFEVFAGASAQGHYRCD
CCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEE
LARRPGRAARQARLQVYWQAVQLQPGRNDQTHKGQPPVSVWVVRAWEVDPPAGEEGIDWW
CCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEE
LLSSQPVTTLEQALACVHRYTLRWLIERYHYILKSGCAVEQLQLETAQRLERALAVYCIV
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AWRLLQLTYQSRSEPELPCTVALAQHEWQALYCRMTQSRQLPEEAPSLRQAVRWIAQLGG
HHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
FVGRKGDGEPGVKTLWRGWQRLEDLAAGWLIARSAMAGDGNEDVGNG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PTIDPWTEHELQHLDLGDARRHTRLKQLLSSLARQPHASLPQACEDAAALKAAYRFLDN
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
PQCHPADIRQAHASATAGRLAALPLVLLIQDTTELNFTAHPHTTGLGHLSKPESQGLLVH
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHH
SLLAVRPDAVALGLCWQKVWTRAAQVEHLAARRQKRPQQHKESQRWLDGLAAAKGYVPPG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
GEAVLIGDRESDMFGLFAAPRPPQLHLLVRAARQRRLATPKTLLFEVFAGASAQGHYRCD
CCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEE
LARRPGRAARQARLQVYWQAVQLQPGRNDQTHKGQPPVSVWVVRAWEVDPPAGEEGIDWW
CCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEE
LLSSQPVTTLEQALACVHRYTLRWLIERYHYILKSGCAVEQLQLETAQRLERALAVYCIV
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AWRLLQLTYQSRSEPELPCTVALAQHEWQALYCRMTQSRQLPEEAPSLRQAVRWIAQLGG
HHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
FVGRKGDGEPGVKTLWRGWQRLEDLAAGWLIARSAMAGDGNEDVGNG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]