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The map label for this gene is slyD [H]

Identifier: 34498284

GI number: 34498284

Start: 3081173

End: 3081649

Strand: Reverse

Name: slyD [H]

Synonym: CV_2829

Alternate gene names: 34498284

Gene position: 3081649-3081173 (Counterclockwise)

Preceding gene: 34498285

Following gene: 34498283

Centisome position: 64.86

GC content: 61.43

Gene sequence:

>477_bases
ATGAAAATCACCGCCAACACCGCCGTCACCCTGAGAATGAAAGTCACCGACAGCCAGGGTCTGGTTTACGACGATGGCAA
GAACCCGGTTTCTTACCTGCACGGCGATTACGACAATCTCTTCCCCAAGCTGGAAGCCGCGCTTGAAGGCCAGGAAGCAG
GGTTTCAAACCACGCTGGAACTGGCTAGCGAAGACGCCTTCGGCGAACGCGACGAAGCCCTGGTCACCACCATGCCCAAG
GCCGAATTCCCGCCCGGCGTCAAAGTAGGCGGCCAGATCCCGCGCCCCGGCCCGGATGGCCAGCGGCGCTACTACTTCGT
CACCAAGATCAAAGGCCCGACGGTATTGCTGGACGGCAACCACCCGCTTTGTGGCAAGACCCTGCGCTTCGCCATCAAGG
TGTTGGACGTGCGGGAGGCCACCGCCGAGGAAATCGCCCACCAGCATGTGCATGGCGAGCACGGGCATCAGCACTGA

Upstream 100 bases:

>100_bases
GGTGGCAAGCGCATAAGCGTTGAGCAAACCAGGGGGATCTGGTTGACACTCCCGCGCCGAACTGAGACCCTTCCCGCTTC
AATTTATTTTGCTGCCCGCC

Downstream 100 bases:

>100_bases
CATCGTCCGTCTCCCGTAAAAAAAGCCCTCCCCATCCGGGAGGGCTTTGTTTTGCCCGATTCTTGCCTGCCATTGGGAGT
GGGAGACCTGCTCACGAGCC

Product: fkbp-type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Metallochaperone slyD [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKITANTAVTLRMKVTDSQGLVYDDGKNPVSYLHGDYDNLFPKLEAALEGQEAGFQTTLELASEDAFGERDEALVTTMPK
AEFPPGVKVGGQIPRPGPDGQRRYYFVTKIKGPTVLLDGNHPLCGKTLRFAIKVLDVREATAEEIAHQHVHGEHGHQH

Sequences:

>Translated_158_residues
MKITANTAVTLRMKVTDSQGLVYDDGKNPVSYLHGDYDNLFPKLEAALEGQEAGFQTTLELASEDAFGERDEALVTTMPK
AEFPPGVKVGGQIPRPGPDGQRRYYFVTKIKGPTVLLDGNHPLCGKTLRFAIKVLDVREATAEEIAHQHVHGEHGHQH
>Mature_158_residues
MKITANTAVTLRMKVTDSQGLVYDDGKNPVSYLHGDYDNLFPKLEAALEGQEAGFQTTLELASEDAFGERDEALVTTMPK
AEFPPGVKVGGQIPRPGPDGQRRYYFVTKIKGPTVLLDGNHPLCGKTLRFAIKVLDVREATAEEIAHQHVHGEHGHQH

Specific function: Also involved in hydrogenase metallocenter assembly, probably by participating in the nickel insertion step. This function in hydrogenase biosynthesis requires chaperone activity and the presence of the metal-binding domain, but not PPIase activity [H]

COG id: COG1047

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerases 2

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789748, Length=153, Percent_Identity=35.2941176470588, Blast_Score=80, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179 [H]

Pfam domain/function: PF00254 FKBP_C [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 17362; Mature: 17362

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITANTAVTLRMKVTDSQGLVYDDGKNPVSYLHGDYDNLFPKLEAALEGQEAGFQTTLE
CEEECCEEEEEEEEEECCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHH
LASEDAFGERDEALVTTMPKAEFPPGVKVGGQIPRPGPDGQRRYYFVTKIKGPTVLLDGN
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCC
HPLCGKTLRFAIKVLDVREATAEEIAHQHVHGEHGHQH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKITANTAVTLRMKVTDSQGLVYDDGKNPVSYLHGDYDNLFPKLEAALEGQEAGFQTTLE
CEEECCEEEEEEEEEECCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHH
LASEDAFGERDEALVTTMPKAEFPPGVKVGGQIPRPGPDGQRRYYFVTKIKGPTVLLDGN
HHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCC
HPLCGKTLRFAIKVLDVREATAEEIAHQHVHGEHGHQH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]