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The map label for this gene is hemL [H]

Identifier: 34498274

GI number: 34498274

Start: 3069549

End: 3070910

Strand: Reverse

Name: hemL [H]

Synonym: CV_2819

Alternate gene names: 34498274

Gene position: 3070910-3069549 (Counterclockwise)

Preceding gene: 34498275

Following gene: 34498273

Centisome position: 64.64

GC content: 70.78

Gene sequence:

>1362_bases
ATGGCGATTCGGCACGGCATTGATCTGGACAAGGCGCGCGCGCTGCATGCGCGCGAGACGGCGGATTTCCTGGCGCGCCG
CCCGCGCTCCCTGGCGCTGGCGGAGCGGGCCGGCGGGCATTTGCTGTTCGGCGTGCCGCTGCACTGGATGGACGATTGGC
CGACGCCGCGCTCACTGTTCCTGGCCGAGGCGCGGGACGCGAGGCTGACCGATGTGGATGGCCATGTCTACGCCGACTTC
TGCCTGGGCGACACCGGCGCGATGTTTGGCCACTCGCCGGCGCCGGTGGCGCAGGCCATCGCCCTAGAGTCGGCACGCGG
CATGACCGCGATGCTGCCCGGCGAGGACGCGCTGTGGGTGGCGGAGGAGCTGTCGCGGCGCTTCGGCCTGCCGGTGTGGC
AGTTCGCGCTGTCGGCCAGCGACGCCAACCGCTTCGCCATCCGCTGGGCGCGGGCGGTCACCGGCCGCGACCGCATCCTG
GTGTTCAACGGCTGCTACCACGGCACGGTGGATGATGTCTTCGTCGACCTGGCGGACGGGGAGGCGAGGCCGCGCGCCAG
TTTGCTGGGCCAGGTCTACGATCTGACCGAGCATACCCGGGTGGCGGAGTTCAACGACCTGGAGGGGCTGGAGGCGGCGC
TGGCCGATGGCCGCGTCGCCTGCGTGCTGGCCGAGCCGGCGATGACCAATATCGGCATGGTGCTGCCCGATCCGGGCTAC
TGGGAGCAGGCGCAGGCCATCATCCGCCGCCACGGCAGCCTGTTGCTGCTGGACGAAACCCATACCATCAGCTGCGGCCC
GGGCGGCTATACCGCCGCGCACGGCTTGCGGCCGGACATGCTGGTGCTGGGCAAACCATTGGCCGGCGGCTTGCCGGCCG
CCGCCTACGGCTTCAGCGCCGATCTGGCTGCGCGGGCGCAGCAGGCCAAGCGCGCCGCGCCGCCCGGCCATTCCGGCATC
GGCACCACGCTGAGCGCCAACCGGCTGGCCTGCGCGGCGATGCGCGCCAACCTGTCCCAGGTGATGACGGATGACAATTA
CCGCATCATGCTGGAGCGCGCCGGCCGGCTGGCGCAGGGCCTGCGCGAGCTGTTCGCCCGCTTCGCCTTGCCCTGGTGCG
TGACGCAGCTGGGCGCGCGCTGCGAGTTCCAGTTCGCCGCCAGGCCGCCGCGCAACGGCAGCGAGGCCGGCGCGGGGCAG
GACGAGGAGCTGGAGCGCTATATTCATCTTTACTTACTGAACCGCGGCCTGCTGATCACGCCCTTCCACAATATGCTGCT
GCTCAGCCCGCATACCGAGGATGCCGACGCGCAGCGCCTGCTGGAGGTCTTCGGCGAATGGCTGGAGCAGGCGAGGGCCT
GA

Upstream 100 bases:

>100_bases
TTGGCCCGGAGCGTTTTTCCTCCGGGCCTTTTGTCGTGGTGGATCATGCCCGGCGGAATTTGGCATACTGGCCGCATCCC
GCGCGACAGGAGCTGCGAGC

Downstream 100 bases:

>100_bases
GCGTCGCTGAGAAAACCCCTGGTCCTGCGTTGCGCCTCCTTGCCGCGCGGCTTGCGCTGTCTGCGTCGGCGCGCCTGGGC
TCAGCTGCTTTGCCCTTCGC

Product: aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MAIRHGIDLDKARALHARETADFLARRPRSLALAERAGGHLLFGVPLHWMDDWPTPRSLFLAEARDARLTDVDGHVYADF
CLGDTGAMFGHSPAPVAQAIALESARGMTAMLPGEDALWVAEELSRRFGLPVWQFALSASDANRFAIRWARAVTGRDRIL
VFNGCYHGTVDDVFVDLADGEARPRASLLGQVYDLTEHTRVAEFNDLEGLEAALADGRVACVLAEPAMTNIGMVLPDPGY
WEQAQAIIRRHGSLLLLDETHTISCGPGGYTAAHGLRPDMLVLGKPLAGGLPAAAYGFSADLAARAQQAKRAAPPGHSGI
GTTLSANRLACAAMRANLSQVMTDDNYRIMLERAGRLAQGLRELFARFALPWCVTQLGARCEFQFAARPPRNGSEAGAGQ
DEELERYIHLYLLNRGLLITPFHNMLLLSPHTEDADAQRLLEVFGEWLEQARA

Sequences:

>Translated_453_residues
MAIRHGIDLDKARALHARETADFLARRPRSLALAERAGGHLLFGVPLHWMDDWPTPRSLFLAEARDARLTDVDGHVYADF
CLGDTGAMFGHSPAPVAQAIALESARGMTAMLPGEDALWVAEELSRRFGLPVWQFALSASDANRFAIRWARAVTGRDRIL
VFNGCYHGTVDDVFVDLADGEARPRASLLGQVYDLTEHTRVAEFNDLEGLEAALADGRVACVLAEPAMTNIGMVLPDPGY
WEQAQAIIRRHGSLLLLDETHTISCGPGGYTAAHGLRPDMLVLGKPLAGGLPAAAYGFSADLAARAQQAKRAAPPGHSGI
GTTLSANRLACAAMRANLSQVMTDDNYRIMLERAGRLAQGLRELFARFALPWCVTQLGARCEFQFAARPPRNGSEAGAGQ
DEELERYIHLYLLNRGLLITPFHNMLLLSPHTEDADAQRLLEVFGEWLEQARA
>Mature_452_residues
AIRHGIDLDKARALHARETADFLARRPRSLALAERAGGHLLFGVPLHWMDDWPTPRSLFLAEARDARLTDVDGHVYADFC
LGDTGAMFGHSPAPVAQAIALESARGMTAMLPGEDALWVAEELSRRFGLPVWQFALSASDANRFAIRWARAVTGRDRILV
FNGCYHGTVDDVFVDLADGEARPRASLLGQVYDLTEHTRVAEFNDLEGLEAALADGRVACVLAEPAMTNIGMVLPDPGYW
EQAQAIIRRHGSLLLLDETHTISCGPGGYTAAHGLRPDMLVLGKPLAGGLPAAAYGFSADLAARAQQAKRAAPPGHSGIG
TTLSANRLACAAMRANLSQVMTDDNYRIMLERAGRLAQGLRELFARFALPWCVTQLGARCEFQFAARPPRNGSEAGAGQD
EELERYIHLYLLNRGLLITPFHNMLLLSPHTEDADAQRLLEVFGEWLEQARA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=321, Percent_Identity=25.8566978193146, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI284507298, Length=171, Percent_Identity=32.7485380116959, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1786349, Length=372, Percent_Identity=30.1075268817204, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1789016, Length=424, Percent_Identity=25, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789759, Length=322, Percent_Identity=23.9130434782609, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693181, Length=319, Percent_Identity=26.3322884012539, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788044, Length=346, Percent_Identity=26.5895953757225, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787560, Length=400, Percent_Identity=25.5, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25144271, Length=296, Percent_Identity=25.6756756756757, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25144274, Length=142, Percent_Identity=33.8028169014084, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 49404; Mature: 49273

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRHGIDLDKARALHARETADFLARRPRSLALAERAGGHLLFGVPLHWMDDWPTPRSLF
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCEEECCCCCCCHHEE
LAEARDARLTDVDGHVYADFCLGDTGAMFGHSPAPVAQAIALESARGMTAMLPGEDALWV
EECCCCCEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHH
AEELSRRFGLPVWQFALSASDANRFAIRWARAVTGRDRILVFNGCYHGTVDDVFVDLADG
HHHHHHHHCCHHHHHHHCCCCCCCEEEEEHHHHCCCCEEEEEECCCCCCHHHEEEECCCC
EARPRASLLGQVYDLTEHTRVAEFNDLEGLEAALADGRVACVLAEPAMTNIGMVLPDPGY
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHCCEECCCCCC
WEQAQAIIRRHGSLLLLDETHTISCGPGGYTAAHGLRPDMLVLGKPLAGGLPAAAYGFSA
HHHHHHHHHHCCCEEEEECCCEEEECCCCCCHHCCCCCCEEEECCCCCCCCCHHHHCCCH
DLAARAQQAKRAAPPGHSGIGTTLSANRLACAAMRANLSQVMTDDNYRIMLERAGRLAQG
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
LRELFARFALPWCVTQLGARCEFQFAARPPRNGSEAGAGQDEELERYIHLYLLNRGLLIT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
PFHNMLLLSPHTEDADAQRLLEVFGEWLEQARA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIRHGIDLDKARALHARETADFLARRPRSLALAERAGGHLLFGVPLHWMDDWPTPRSLF
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCEEECCCCCCCHHEE
LAEARDARLTDVDGHVYADFCLGDTGAMFGHSPAPVAQAIALESARGMTAMLPGEDALWV
EECCCCCEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHH
AEELSRRFGLPVWQFALSASDANRFAIRWARAVTGRDRILVFNGCYHGTVDDVFVDLADG
HHHHHHHHCCHHHHHHHCCCCCCCEEEEEHHHHCCCCEEEEEECCCCCCHHHEEEECCCC
EARPRASLLGQVYDLTEHTRVAEFNDLEGLEAALADGRVACVLAEPAMTNIGMVLPDPGY
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHCCEECCCCCC
WEQAQAIIRRHGSLLLLDETHTISCGPGGYTAAHGLRPDMLVLGKPLAGGLPAAAYGFSA
HHHHHHHHHHCCCEEEEECCCEEEECCCCCCHHCCCCCCEEEECCCCCCCCCHHHHCCCH
DLAARAQQAKRAAPPGHSGIGTTLSANRLACAAMRANLSQVMTDDNYRIMLERAGRLAQG
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
LRELFARFALPWCVTQLGARCEFQFAARPPRNGSEAGAGQDEELERYIHLYLLNRGLLIT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEE
PFHNMLLLSPHTEDADAQRLLEVFGEWLEQARA
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA