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The map label for this gene is leuD

Identifier: 34498237

GI number: 34498237

Start: 3017011

End: 3017649

Strand: Reverse

Name: leuD

Synonym: CV_2782

Alternate gene names: 34498237

Gene position: 3017649-3017011 (Counterclockwise)

Preceding gene: 34498238

Following gene: 34498236

Centisome position: 63.52

GC content: 63.69

Gene sequence:

>639_bases
ATGAAAGCATTCACCACTTTGCAGGGCCTGGTCTGTCCGCTGGACAGGGCCAATGTCGATACCGACGCCATCATTCCCAA
GCAGTTCCTGAAGTCGATCAAGCGGTCCGGCTTCGGGCCTAACCTGTTCGACGAATGGCGTTACCTGGATCATGGCGAGC
CGGGCATGGACAACGCCAAGCGTCCGCTGAATCCGGACTTCGTGCTGAACCAGCCGCGCTACCAGGGCGCGCAAGTGCTG
CTGGCGCGCGAGAACTTCGGCTGCGGCTCCAGCCGCGAGCACGCGCCGTGGGCGCTGGACGACCAGGGTTTCCGCGTGGT
GATCGCGCCCAGCTTCGCCGACATCTTCTTCAACAACTGCTACAAGAACGGCCTGCTGCCCATCGTGCTGGCGGCGGACA
TCGTCGACCAGCTGTTCCAGGAGTGCGAGGCGGCGCCGGGCTACAGCCTGAAAGTGGACCTGGCGGCGCAAACGGTGTTC
ACGCCGTCCGGACAGGCCTTCCTGTTTGACATCACCGAACACCGCAAGCACTGCCTGCTGGGCGGCCTGGACGAAATCGG
CCTGACCCTGCAGCATGCCGACGAGATCAAGGCTTTCGAAGCCAGGCGCCGAGTCGAGCAGCCCTGGCTGTTCGCCTGA

Upstream 100 bases:

>100_bases
AAGGCGGGACAGGCGGTCGGCAACGCGGTGGAGAAGAGCGGCGAAGCGATCAAGCGCACCGCCGAGTGATCCCCGCGCCA
TCCTGAACAGGGAGCGCATT

Downstream 100 bases:

>100_bases
GGCGATGCCATGGCGTACCGCTGCCCGGTAGCCTGTCCGCGTCATCAGCACGGATAAAACATCCGCGCGGCGCGGGGCTG
CCGGCGAAGGCGCCGGTTCA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKAFTTLQGLVCPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDHGEPGMDNAKRPLNPDFVLNQPRYQGAQVL
LARENFGCGSSREHAPWALDDQGFRVVIAPSFADIFFNNCYKNGLLPIVLAADIVDQLFQECEAAPGYSLKVDLAAQTVF
TPSGQAFLFDITEHRKHCLLGGLDEIGLTLQHADEIKAFEARRRVEQPWLFA

Sequences:

>Translated_212_residues
MKAFTTLQGLVCPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDHGEPGMDNAKRPLNPDFVLNQPRYQGAQVL
LARENFGCGSSREHAPWALDDQGFRVVIAPSFADIFFNNCYKNGLLPIVLAADIVDQLFQECEAAPGYSLKVDLAAQTVF
TPSGQAFLFDITEHRKHCLLGGLDEIGLTLQHADEIKAFEARRRVEQPWLFA
>Mature_212_residues
MKAFTTLQGLVCPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDHGEPGMDNAKRPLNPDFVLNQPRYQGAQVL
LARENFGCGSSREHAPWALDDQGFRVVIAPSFADIFFNNCYKNGLLPIVLAADIVDQLFQECEAAPGYSLKVDLAAQTVF
TPSGQAFLFDITEHRKHCLLGGLDEIGLTLQHADEIKAFEARRRVEQPWLFA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=202, Percent_Identity=57.9207920792079, Blast_Score=234, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=50, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD2_CHRVO (Q7NUB8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902452.1
- ProteinModelPortal:   Q7NUB8
- GeneID:   2548220
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2782
- NMPDR:   fig|243365.1.peg.2782
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q7NUB8
- ProtClustDB:   PRK01641
- BioCyc:   CVIO243365:CV_2782-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 23768; Mature: 23768

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFTTLQGLVCPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDHGEPGMDNAK
CCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCC
RPLNPDFVLNQPRYQGAQVLLARENFGCGSSREHAPWALDDQGFRVVIAPSFADIFFNNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHH
YKNGLLPIVLAADIVDQLFQECEAAPGYSLKVDLAAQTVFTPSGQAFLFDITEHRKHCLL
HCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEEEEEHHHHHHHHH
GGLDEIGLTLQHADEIKAFEARRRVEQPWLFA
CCHHHHCEEHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKAFTTLQGLVCPLDRANVDTDAIIPKQFLKSIKRSGFGPNLFDEWRYLDHGEPGMDNAK
CCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCC
RPLNPDFVLNQPRYQGAQVLLARENFGCGSSREHAPWALDDQGFRVVIAPSFADIFFNNC
CCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHH
YKNGLLPIVLAADIVDQLFQECEAAPGYSLKVDLAAQTVFTPSGQAFLFDITEHRKHCLL
HCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEEEEEHHHHHHHHH
GGLDEIGLTLQHADEIKAFEARRRVEQPWLFA
CCHHHHCEEHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782