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The map label for this gene is truA

Identifier: 34498219

GI number: 34498219

Start: 2999847

End: 3000635

Strand: Reverse

Name: truA

Synonym: CV_2764

Alternate gene names: 34498219

Gene position: 3000635-2999847 (Counterclockwise)

Preceding gene: 34498220

Following gene: 34498218

Centisome position: 63.16

GC content: 66.79

Gene sequence:

>789_bases
ATGAGAATCGCGCTCGGCATTGAGTATGACGGTCGGGCGTTCGCCGGCTGGCAGAGTCAGCCGCACGGCAACACGGTACA
GGACAGGCTGAACAGGGCGCTGGGGCAGATCGCCGGCAACCGCGATGTGGTCACCCACGCCGCCGGCCGCACCGATGCCG
GCGTGCATGCCGCGATGCAGGTGGTTCACTTCGATACCGACGCATCCCGTCCGCTCAACGCCTGGGTGAGAGGCGTGAAC
GCCTTGCTGCCGCCGGAAGTGGCCGTGGTATGGGCGCGAGAGGTCGGGGACGATTTCCATGCCCGGTTTTCCGCGTTTTC
CCGCAGCTACAGCTATTTTCTGCTGACCCACCCGGTCCGCTCCTGCCTGTTGGCGGGGAAGGTGGGCTGGTATCACCAGG
CGCTGGATGTGGCGGCGATGAGGGAGGCGGCGGCCGGGCTGCTGGGGCGGCACGACTTTTCCAGTTTCCGCGCGTCGGAA
TGCCAGGCCAAGTCGCCGGTCAAGGATTTGCAGCGGCTGGACATCATCGAGGCGGATGGCTTGCTGCGCTTCGATCTGCA
CGCCGACGCCTTTCTGCATCACATGGTGCGCAATATCGTCGGCGCCCTGCTGTACGTCGGCAAGGGCGCGCTGAGCCCCG
CCGACATGCAGTCGCTGCTGGCGGCGCGGGACCGCACCAGCGCGCCGCCCACCTTCATGCCGGACGGGCTGTATCTGACC
GGCGTCGGTTATCCGGATGAGTTTTCCCTGCCGTCCCGCTGCGAGGCGGCGCGGCTCAGATTGCGCTGA

Upstream 100 bases:

>100_bases
AGCGTGTGGCGCAAACCGGGCAGGATAGGATAAAGTGGGGTTTTATCGCCCTGGCGGGGTTTTGTCCTGCAAGGGGCTTG
CAATGAATGGATGAGTGGGT

Downstream 100 bases:

>100_bases
AATGCAACGCTTTATAGGGCGAACATAGAAAATCGTGGTCAGAATCAAAGTTTGTGGCATCACCAGGCCGGAAGACGGCG
CAGAGGCGGCCCGCTTGGGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIALGIEYDGRAFAGWQSQPHGNTVQDRLNRALGQIAGNRDVVTHAAGRTDAGVHAAMQVVHFDTDASRPLNAWVRGVN
ALLPPEVAVVWAREVGDDFHARFSAFSRSYSYFLLTHPVRSCLLAGKVGWYHQALDVAAMREAAAGLLGRHDFSSFRASE
CQAKSPVKDLQRLDIIEADGLLRFDLHADAFLHHMVRNIVGALLYVGKGALSPADMQSLLAARDRTSAPPTFMPDGLYLT
GVGYPDEFSLPSRCEAARLRLR

Sequences:

>Translated_262_residues
MRIALGIEYDGRAFAGWQSQPHGNTVQDRLNRALGQIAGNRDVVTHAAGRTDAGVHAAMQVVHFDTDASRPLNAWVRGVN
ALLPPEVAVVWAREVGDDFHARFSAFSRSYSYFLLTHPVRSCLLAGKVGWYHQALDVAAMREAAAGLLGRHDFSSFRASE
CQAKSPVKDLQRLDIIEADGLLRFDLHADAFLHHMVRNIVGALLYVGKGALSPADMQSLLAARDRTSAPPTFMPDGLYLT
GVGYPDEFSLPSRCEAARLRLR
>Mature_262_residues
MRIALGIEYDGRAFAGWQSQPHGNTVQDRLNRALGQIAGNRDVVTHAAGRTDAGVHAAMQVVHFDTDASRPLNAWVRGVN
ALLPPEVAVVWAREVGDDFHARFSAFSRSYSYFLLTHPVRSCLLAGKVGWYHQALDVAAMREAAAGLLGRHDFSSFRASE
CQAKSPVKDLQRLDIIEADGLLRFDLHADAFLHHMVRNIVGALLYVGKGALSPADMQSLLAARDRTSAPPTFMPDGLYLT
GVGYPDEFSLPSRCEAARLRLR

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=269, Percent_Identity=31.9702602230483, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI199559785, Length=270, Percent_Identity=25.5555555555556, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=47.0119521912351, Blast_Score=234, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CHRVO (Q7NUD6)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902434.1
- ProteinModelPortal:   Q7NUD6
- SMR:   Q7NUD6
- GeneID:   2547922
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2764
- NMPDR:   fig|243365.1.peg.2764
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- PhylomeDB:   Q7NUD6
- ProtClustDB:   PRK00021
- BioCyc:   CVIO243365:CV_2764-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28734; Mature: 28734

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIEYDGRAFAGWQSQPHGNTVQDRLNRALGQIAGNRDVVTHAAGRTDAGVHAAMQ
CEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
VVHFDTDASRPLNAWVRGVNALLPPEVAVVWAREVGDDFHARFSAFSRSYSYFLLTHPVR
HEEECCCCCCHHHHHHHHHHCCCCCHHEEEEHHHHCCHHHHHHHHHHCCCEEEEEHHHHH
SCLLAGKVGWYHQALDVAAMREAAAGLLGRHDFSSFRASECQAKSPVKDLQRLDIIEADG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCEECCCC
LLRFDLHADAFLHHMVRNIVGALLYVGKGALSPADMQSLLAARDRTSAPPTFMPDGLYLT
EEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GVGYPDEFSLPSRCEAARLRLR
ECCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MRIALGIEYDGRAFAGWQSQPHGNTVQDRLNRALGQIAGNRDVVTHAAGRTDAGVHAAMQ
CEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHH
VVHFDTDASRPLNAWVRGVNALLPPEVAVVWAREVGDDFHARFSAFSRSYSYFLLTHPVR
HEEECCCCCCHHHHHHHHHHCCCCCHHEEEEHHHHCCHHHHHHHHHHCCCEEEEEHHHHH
SCLLAGKVGWYHQALDVAAMREAAAGLLGRHDFSSFRASECQAKSPVKDLQRLDIIEADG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCEECCCC
LLRFDLHADAFLHHMVRNIVGALLYVGKGALSPADMQSLLAARDRTSAPPTFMPDGLYLT
EEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GVGYPDEFSLPSRCEAARLRLR
ECCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782