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The map label for this gene is narH [H]

Identifier: 34497997

GI number: 34497997

Start: 2731396

End: 2732946

Strand: Reverse

Name: narH [H]

Synonym: CV_2542

Alternate gene names: 34497997

Gene position: 2732946-2731396 (Counterclockwise)

Preceding gene: 34497998

Following gene: 34497996

Centisome position: 57.52

GC content: 62.41

Gene sequence:

>1551_bases
ATGAAGATACGCGCGCAAATCGGCATGGTGCTGAACCTGGACAAGTGCATCGGTTGCCACACCTGTTCGGTCACCTGCAA
GAACGTATGGACCAGCCGCGACGGCATCGAGTACGCCTGGTTCAACAATGTGGAGACCAAGCCCGGCATCGGCTACCCCA
AGGACTGGGAAAATCAGGACAAGTGGCAGGGCGGCTGGAAGCGCCGCGCCGACGGCAAGCTGGAGCCGCGCCAGGGCGGC
CGGCTTAAGATACTGGCCAATATCTTCGCCAACCCCAATCTGCCGGCGATAGACGACTACTACGAGCCGTTCACCTACGA
CTACGAGCATCTGCAGAACGCGCCGCAGATGCAGACGCCGCCGACCGCGCGGCCGGTGTCGGTGCTGACCGGCAAGAAGA
TGGACAAGATCGAGTGGGGCCCGAACTGGGAGGACGACCTGGGCGGCGAGTTCTCCAAGCGCGCCAAGGATGCTTTGTTT
GAAGGCATCCAGAAGGAGATGTACTCCGCCTTCGAGAACACCTTCATGATGTATCTGCCGCGGCTGTGCGAACACTGCCT
GAACCCGGCCTGCGTCGCGTCCTGTCCGTCCGGCTCCATCTACAAGCGCGAAGACGACGGCATCGTCCTGATCGACCAGG
ACAAGTGCCGCGGTTGGCGGATGTGCGTGTCAGGCTGTCCGTACAAAAAGATTTATTACAACTGGACGTCCGGCAAGGCC
GAGAAGTGCATCTTCTGCTACCCGCGCATCGAGTCCGGCCAGCCTACCGTGTGCTCGGAAACCTGCGTCGGCCGCATCCG
TTACCTGGGCGTGCTGCTGTACGACGCCGACAAGATCGAACAGGCGGCGGCGGTGGAAGATCCGCAGGATCTTTACCAGT
CGCAGCTGGACCTGTTCCTGGATCCGAACGACCCTGCCATCGCGGCCGAGGCGCTGAAGCAGGGCATCCCGCAAAGCTGG
CTGGACGCCGCGAAGCGCTCGCCGGTGTACAAGATGGCGATGGAGTGGAAGGTGGCCTTCCCGCTGCATCCGGAATACCG
GACGCTGCCGATGGTGTGGTACATCCCGCCGCTGTCGCCGATCCAGAGCGCGATGGAGAACGGCCTGATAGGCGAGGGCG
GCATCATCCCCGACGTGCAGAGCCTGCGCATACCGATCAAGTATCTGGCCAACCTGCTGACGGCCGGCAAGGAAGAGCCG
GTGGTCAAGGCGCTGGAAACCATGGTGGCGATGCGCCGCTACATGCGCAAGAAGAGCGTGGAGAAGGTCTGCGACGCGGC
TACCCTGGCCGGTACCCACCTGACCGCAAGCCAGGTGGAAGAGATGTACCAGGTGATGGCCATCGCCAATTACGAAGACC
GCTTCGTGATTCCGTCCAGCCACAAGGAAATGGTGGAGGAGGCGTTCGAGGACAAGGCCAGCTGCGGCTTCAGCTTCGGC
AACGGCTGCTCCGGCGGCGAGTCCGAAGCCAGCCTGTTCGGCAAGAAGAAGGCCACGCCCATCGTGTTCCACGACATGCG
GCGCGACCGCAAGTCCAACGCCGGCAACTGA

Upstream 100 bases:

>100_bases
TCAACTACTACGGCACGGTGGGCGCCAACCGCGACGAGTTCGTGGTGGTGCGCAAGATGGACAAGGTGGACTGGATGGAC
CAGCCCGCGGGAGACAAGCA

Downstream 100 bases:

>100_bases
GGGAGACCGATATGCGCATCCACGCCATTCTGTCCGCGCTGTTGTGCTACCCGGAGGCTGAGCTGGTCGCCGCGGTGGAC
GAGATCCGCGCCGAGGCGGC

Product: nitrate reductase

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKDWENQDKWQGGWKRRADGKLEPRQGG
RLKILANIFANPNLPAIDDYYEPFTYDYEHLQNAPQMQTPPTARPVSVLTGKKMDKIEWGPNWEDDLGGEFSKRAKDALF
EGIQKEMYSAFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWTSGKA
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADKIEQAAAVEDPQDLYQSQLDLFLDPNDPAIAAEALKQGIPQSW
LDAAKRSPVYKMAMEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAMENGLIGEGGIIPDVQSLRIPIKYLANLLTAGKEEP
VVKALETMVAMRRYMRKKSVEKVCDAATLAGTHLTASQVEEMYQVMAIANYEDRFVIPSSHKEMVEEAFEDKASCGFSFG
NGCSGGESEASLFGKKKATPIVFHDMRRDRKSNAGN

Sequences:

>Translated_516_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKDWENQDKWQGGWKRRADGKLEPRQGG
RLKILANIFANPNLPAIDDYYEPFTYDYEHLQNAPQMQTPPTARPVSVLTGKKMDKIEWGPNWEDDLGGEFSKRAKDALF
EGIQKEMYSAFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWTSGKA
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADKIEQAAAVEDPQDLYQSQLDLFLDPNDPAIAAEALKQGIPQSW
LDAAKRSPVYKMAMEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAMENGLIGEGGIIPDVQSLRIPIKYLANLLTAGKEEP
VVKALETMVAMRRYMRKKSVEKVCDAATLAGTHLTASQVEEMYQVMAIANYEDRFVIPSSHKEMVEEAFEDKASCGFSFG
NGCSGGESEASLFGKKKATPIVFHDMRRDRKSNAGN
>Mature_516_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKDWENQDKWQGGWKRRADGKLEPRQGG
RLKILANIFANPNLPAIDDYYEPFTYDYEHLQNAPQMQTPPTARPVSVLTGKKMDKIEWGPNWEDDLGGEFSKRAKDALF
EGIQKEMYSAFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWTSGKA
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADKIEQAAAVEDPQDLYQSQLDLFLDPNDPAIAAEALKQGIPQSW
LDAAKRSPVYKMAMEWKVAFPLHPEYRTLPMVWYIPPLSPIQSAMENGLIGEGGIIPDVQSLRIPIKYLANLLTAGKEEP
VVKALETMVAMRRYMRKKSVEKVCDAATLAGTHLTASQVEEMYQVMAIANYEDRFVIPSSHKEMVEEAFEDKASCGFSFG
NGCSGGESEASLFGKKKATPIVFHDMRRDRKSNAGN

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=502, Percent_Identity=69.5219123505976, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=508, Percent_Identity=68.3070866141732, Blast_Score=773, Evalue=0.0,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=37.8787878787879, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787872, Length=159, Percent_Identity=35.8490566037736, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367345, Length=120, Percent_Identity=28.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=36.0824742268041, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58212; Mature: 58212

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKDWENQD
CEEHEECCEEEEHHHHHCCCCCEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRRADGKLEPRQGGRLKILANIFANPNLPAIDDYYEPFTYDYEHLQNAPQMQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGKKMDKIEWGPNWEDDLGGEFSKRAKDALFEGIQKEMYSAFENTFMMYLP
CCCCCHHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWTSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADKIEQAAAVEDPQDLYQSQLDLFL
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHHCCEEE
DPNDPAIAAEALKQGIPQSWLDAAKRSPVYKMAMEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCHH
IQSAMENGLIGEGGIIPDVQSLRIPIKYLANLLTAGKEEPVVKALETMVAMRRYMRKKSV
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EKVCDAATLAGTHLTASQVEEMYQVMAIANYEDRFVIPSSHKEMVEEAFEDKASCGFSFG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCC
NGCSGGESEASLFGKKKATPIVFHDMRRDRKSNAGN
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRDGIEYAWFNNVETKPGIGYPKDWENQD
CEEHEECCEEEEHHHHHCCCCCEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRRADGKLEPRQGGRLKILANIFANPNLPAIDDYYEPFTYDYEHLQNAPQMQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGKKMDKIEWGPNWEDDLGGEFSKRAKDALFEGIQKEMYSAFENTFMMYLP
CCCCCHHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWTSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADKIEQAAAVEDPQDLYQSQLDLFL
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHHCCEEE
DPNDPAIAAEALKQGIPQSWLDAAKRSPVYKMAMEWKVAFPLHPEYRTLPMVWYIPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCHH
IQSAMENGLIGEGGIIPDVQSLRIPIKYLANLLTAGKEEPVVKALETMVAMRRYMRKKSV
HHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
EKVCDAATLAGTHLTASQVEEMYQVMAIANYEDRFVIPSSHKEMVEEAFEDKASCGFSFG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCC
NGCSGGESEASLFGKKKATPIVFHDMRRDRKSNAGN
CCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]