Click here to switch to the map view.

The map label for this gene is pntA-1 [H]

Identifier: 33863436

GI number: 33863436

Start: 1257816

End: 1258958

Strand: Reverse

Name: pntA-1 [H]

Synonym: PMT1166

Alternate gene names: 33863436

Gene position: 1258958-1257816 (Counterclockwise)

Preceding gene: 33863439

Following gene: 33863435

Centisome position: 52.22

GC content: 56.08

Gene sequence:

>1143_bases
TTGCCCAGACTCTTAATTCAGCTGGAATCGGCGATGGGAGAAACCCGTGTTGCGGCTTCGCCGGACACGGTGAAAAAGTT
TCTTTCTCTGGGCAGCAAGGTTGCCATTGAGCGGGGTGCAGGCCTTGCCGCAGGTTTTGTTGATGCTGTTTATGGAAACG
TTGGTGCTGAGCTGGTCACCCCTGGGGATGAACAGTTCTGGGGTCAGACGGATTTACTCCTCTGTGTGCATGCACCGTCT
GAAATCTCATTGCAGAAGCTGCGGCCTGGAGCCACCATTGTTGGCTTGTTGGCCCCTTACAGCAATTCTGCTTTGGCGGC
GGCGCTGAAATCGGCCAAGCTCTCAGCGATGGCCCTAGAGCTGCTGCCGAGAATTAGTCGGGCACAGTCTGCGGATGCTC
TGTCCTCTCAGGCCAATATTGCTGGCTACAAGGCGGTGCTGTTGGCTGCAGCTGCCCTCGATCGTTACTTCCCGATGTTG
ATGACGGCAGCTGGCACAGTGCAGCCAGCACGGGTCGTGGTATTGGGTGCTGGTGTGGCTGGATTGCAGGCAGTTGCTAC
GGCAAAGCGGCTGGGTGCGGTGGTCTACGTCAGTGATGTTCGCTCTGCCGTGAAGGAGCAGGTGGAGTCACTTGGAGCAC
GCTTTATCGATCCCCCTGAGATTGAGCAGAGGCCAGATGAGTCTGGTGGCTATGCCAAGCAGGCTTCTGAGGCTTTCCTG
GCGGCTCAGCGTGAGCAGCTCTCCAATCAGTTGGCGCAGGCCGATGTGGTGATCTGTACTGCTCAGGTGCCTGGGAGAAA
AGCTCCCAGATTGATCAGTGATCTCATGCTCGATCGCATGCGCCCAGGAGCTGTGGTGGTCGATCTTGCCGTTGAACAGG
GTGGAAATTGTGCGAATACGCGTCCTGGTGAAACAGTCGATCGCAACGGCGTGAAGTTGATCGGTGCCTCCGATCTCCCT
TGCAGTGTGCCCAATCACGCTAGTGCTCTTTACTCCCGCAATTTGGTGGCTCTGTTGGAGCCCGTTTTGAAGGACGGCCA
GCTCAGTCTCAACATTGAGGATGAGTTAATTGCCGGATGCCTGATCAGTCACAACGGTGAGATTCGCCATAACAATGTGT
TCAACATGGGAGGGTCCAACTGA

Upstream 100 bases:

>100_bases
TCATAGCTAAAAGTTCAGCTAAAAAAACTTTTCTTCAGGAAAGCTCCCGGTATGGGGAGGTCGACCCCGTAATCTCGCCT
GGTTAAACAAGTTTTCTCTC

Downstream 100 bases:

>100_bases
TGTCGTTCTTGAGTGAGGCCCTTTGGGTGCTGCTGCTTGGCAGCTTGCTTGGCCTTGAACTGATCGGCAAGGTGCCCCCC
ACTTTGCATACGCCTTTGAT

Product: nicotinamide nucleotide transhydrogenase, subunit alpha 1 (A1)

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPRLLIQLESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDAVYGNVGAELVTPGDEQFWGQTDLLLCVHAPS
EISLQKLRPGATIVGLLAPYSNSALAAALKSAKLSAMALELLPRISRAQSADALSSQANIAGYKAVLLAAAALDRYFPML
MTAAGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIDPPEIEQRPDESGGYAKQASEAFL
AAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANTRPGETVDRNGVKLIGASDLP
CSVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGCLISHNGEIRHNNVFNMGGSN

Sequences:

>Translated_380_residues
MPRLLIQLESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDAVYGNVGAELVTPGDEQFWGQTDLLLCVHAPS
EISLQKLRPGATIVGLLAPYSNSALAAALKSAKLSAMALELLPRISRAQSADALSSQANIAGYKAVLLAAAALDRYFPML
MTAAGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIDPPEIEQRPDESGGYAKQASEAFL
AAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANTRPGETVDRNGVKLIGASDLP
CSVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGCLISHNGEIRHNNVFNMGGSN
>Mature_379_residues
PRLLIQLESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDAVYGNVGAELVTPGDEQFWGQTDLLLCVHAPSE
ISLQKLRPGATIVGLLAPYSNSALAAALKSAKLSAMALELLPRISRAQSADALSSQANIAGYKAVLLAAAALDRYFPMLM
TAAGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIDPPEIEQRPDESGGYAKQASEAFLA
AQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANTRPGETVDRNGVKLIGASDLPC
SVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGCLISHNGEIRHNNVFNMGGSN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=359, Percent_Identity=38.9972144846797, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI122939153, Length=359, Percent_Identity=38.9972144846797, Blast_Score=232, Evalue=3e-61,
Organism=Escherichia coli, GI1787887, Length=369, Percent_Identity=44.7154471544715, Blast_Score=298, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17550456, Length=357, Percent_Identity=44.2577030812325, Blast_Score=290, Evalue=9e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39752; Mature: 39621

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLLIQLESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDAVYGNVGAELVT
CCCEEEEEHHHCCCCEECCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCEEEC
PGDEQFWGQTDLLLCVHAPSEISLQKLRPGATIVGLLAPYSNSALAAALKSAKLSAMALE
CCCHHHCCCCCEEEEEECCCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LLPRISRAQSADALSSQANIAGYKAVLLAAAALDRYFPMLMTAAGTVQPARVVVLGAGVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHH
GLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIDPPEIEQRPDESGGYAKQASEAFL
HHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHH
AAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANT
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
RPGETVDRNGVKLIGASDLPCSVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGC
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCEEEEEECHHHHHHH
LISHNGEIRHNNVFNMGGSN
HCCCCCCEEECCEECCCCCC
>Mature Secondary Structure 
PRLLIQLESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDAVYGNVGAELVT
CCEEEEEHHHCCCCEECCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCEEEC
PGDEQFWGQTDLLLCVHAPSEISLQKLRPGATIVGLLAPYSNSALAAALKSAKLSAMALE
CCCHHHCCCCCEEEEEECCCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LLPRISRAQSADALSSQANIAGYKAVLLAAAALDRYFPMLMTAAGTVQPARVVVLGAGVA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHH
GLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIDPPEIEQRPDESGGYAKQASEAFL
HHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHH
AAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANT
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
RPGETVDRNGVKLIGASDLPCSVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGC
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCEEEEEECHHHHHHH
LISHNGEIRHNNVFNMGGSN
HCCCCCCEEECCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]