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The map label for this gene is gyrA [H]

Identifier: 33863395

GI number: 33863395

Start: 1215746

End: 1218373

Strand: Reverse

Name: gyrA [H]

Synonym: PMT1124

Alternate gene names: 33863395

Gene position: 1218373-1215746 (Counterclockwise)

Preceding gene: 33863401

Following gene: 33863394

Centisome position: 50.54

GC content: 53.39

Gene sequence:

>2628_bases
ATGGCGGATCCATTGGGACCCAGTGACGGCGGTCCCGGCGAGTCCGACGATCGGATAATCCAGACGGACCTGCGCATTGA
AATGTCGCGCTCCTATCTGGAGTACGCGATGAGTGTGATCGTTGGGCGTGCGTTGCCCGACGCTCGCGATGGTCTTAAGC
CTGTGCATAGGCGCATCCTTTATGCGATGTATGAGTTGGGCCTGACCAGCGATCGGCCTTACCGAAAGTGTGCCCGAGTT
GTTGGTGAGGTTCTGGGTAAATACCATCCCCATGGGGATACGGCCGTCTACGACGCGCTGGTTCGCATGGCTCAGGATTT
TTCCATGCAAATGCCGTTGATTGATGGTCACGGCAATTTTGGTTCGGTCGATAACGATCCACCTGCGGCCATGCGATACA
CCGAATCGCGTCTTCAGTCGCTTACGACAGACAGTCTGCTTGAGGATATCGAGGCAGAGACAGTTGACTACGCGGACAAC
TTTGATGGCTCCCAACAGGAGCCAATGGTATTGCCAGCACGTATTCCTCAGTTGCTGCTGAATGGCTCCTCAGGGATCGC
CGTTGGCATGGCTACCAATATTCCACCGCACAATCTCAGTGAATTAATCGATGGTCTGCAGGCTCTGATCAGTAATCCCG
AGCTAAGTGATTCTGAGTTGATGACGCTTATCCCTGGGCCGGACTTTCCAACTGGTGGTCAGATTCTTGGTCGTAGTGGT
ATTCGTGAAACCTATCTCACCGGTCGTGGTTCGGTAACGATGCGCGGCGTGGCAAATATTGAGACGATTGAACATCCCGG
TCGAGCAGACCGGGATGCAGTGATCATTACTGAATTGCCCTATCAGACCAATAAAGCGGCGCTGATTGAGCGGATTGCAG
AGATGGTTAATGATAAGAAGCTGGAGGGTATTTCTGATATTCGCGACGAAAGCGATCGCGATGGCATGCGTATTGTCGTG
GAGCTGCGACGTGATGCCTACCCTCAGGTAGTGCTCAATAATCTTTTCAAGCTCACTCCTCTTCAGAGCAACTTTAGTGC
CCATATGTTGGCGCTAGTCAATGGCGAGCCGGTGCTGCTCACCCTGCGCAAGATGCTGCAGGTATTCCTCGATTTTCGCG
TTGATACTGTTGAACGTCGGACCCGCTATTTCCTTCGTAAAGCTGAGGAACGCGACCACATCTTGTTGGGTCTTTTGCTT
GCTCTCGATCAGCTTGATCCAATCATCGCGTTGATTCGTGCTGCCTCTGATACGGCTACAGCTCGTCAACAGTTGCAGGA
GCATCATGGTCTCACGGAGATTCAGGCGGATGCCATCCTGCAGATGCAGTTGCGGCGTTTGACCGCTTTAGAGGCTGACA
AGATTCGTTTGGAACACGAGGATCTTGTCACCAAGATCGCCAATTACAAGGACATCCTTGGCCGACGTGAGCGTGTTTTC
GGTCTAATTGAGGACGAATTGACCAAGCTGCGCGAGAAGCATTGCTTGCCTCGGCGCACCGAGATCCTCGATTTAGGAGG
GGGGCTCGAAGACATCGACTTGATTGCCAACGAGCGATCTGTGGTGTTGCTCACTGAGACCGGTTATCTCAAGCGCATGC
CGGTAAGCGAGTTTGAGGCCACTAGTCGTGGGACACGAGGCAAGGCAGGCACTCGCAGTCAAGGTGAGGAGGCGGTGAAG
CTGTTTATCAGCTGCAATGACCATGACACGTTGCTGTTGTTCAGTGACCGCGGTGTCTCCTATGCCCTTCCTGCTTATCG
TGTGCCTCAGTGCAGCCGCACAGCGAAGGGAACACCTGTCGTACAGCTCTTGCCCATTCCCCGTGAAGAGGCAATTACCT
CCCTTCTGTCAGTTATATCCTTCGATGACGACACCCACTTGTTGATGTTGACGAAGGGTGGCTTTATCAAGCGCACCTCT
CTATCAGCTTTCAGCAAGATTCGTTCCAATGGTTTGATTGCCATTGGTCTGGAGGAGGGTGATGCTCTTACTTGGGTGAG
GTTGGCTGTTCCTGGTGACAGTGTTTTGATTGCTTCTAGGGCTGGAATGACCATTCATTTCCGACTCTGTGATGAGGAGT
TACGACCCTTAGGACGAACTGCTCGTGGCGTGCGTGCCATGAATTTACGCGGCGGCGATTCCTTGGTGAGCATGGATGTA
TTGCCTGTCGAGCTTGCCGATCAGGTCGCCAAGAGTCTTGAGGATGATCAGGATGAAAGCGGCGAGACCGTTGTCGTTAG
TGATGGACCATGGGTGCTTGTTGCTTCCGCTAGTGGACTGGGTAAGCGGGTACCGGTCAATCAGTTCCGTTTGCAGAAAC
GTGCAGGTATGGGTCTGCGTGCCATCAAGTTCAGGCGCGAAGGAGACGAGTTGGTGGGTTTAAGGGTGCTTGGCTACGGA
GAAGAGCTTTTGTTGGTAAGCGAGCGTGGAGTGATTGTGCGTACCAGTGCCGATAAAATTCCTCAGCAGTCAAGGGCTGC
TACTGGTGTTCGGCTTCAGCGCTTGGATGCAGGTGATCGCCTCTCTGAGGTGGTGCTTGTGCCGCCTGATTCTGAGGATC
AGGACCAGCCAGATGATCGATCTGATCCCAGTGAAGTGGCTGGGGTTGACGACGCTCCTTCCAGCTGA

Upstream 100 bases:

>100_bases
TGTATTAAGCCCTAACAGTGCAGAGGCTGGTTTTTGTTGATGCTGGTGGCTCAATTCATCAGCTTCCGAGATAACCTGAT
TATTGGCAACCAGCTCTTTT

Downstream 100 bases:

>100_bases
CATTGACCAGTCGTACGGATGTTTTAGTGATGGGAGCTGGTCCGGCGGCTCTCTGTATCGTTGCTGAGTTAGTCGAGCAA
GGCTTGGCAGTGACTGCCCT

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MADPLGPSDGGPGESDDRIIQTDLRIEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMYELGLTSDRPYRKCARV
VGEVLGKYHPHGDTAVYDALVRMAQDFSMQMPLIDGHGNFGSVDNDPPAAMRYTESRLQSLTTDSLLEDIEAETVDYADN
FDGSQQEPMVLPARIPQLLLNGSSGIAVGMATNIPPHNLSELIDGLQALISNPELSDSELMTLIPGPDFPTGGQILGRSG
IRETYLTGRGSVTMRGVANIETIEHPGRADRDAVIITELPYQTNKAALIERIAEMVNDKKLEGISDIRDESDRDGMRIVV
ELRRDAYPQVVLNNLFKLTPLQSNFSAHMLALVNGEPVLLTLRKMLQVFLDFRVDTVERRTRYFLRKAEERDHILLGLLL
ALDQLDPIIALIRAASDTATARQQLQEHHGLTEIQADAILQMQLRRLTALEADKIRLEHEDLVTKIANYKDILGRRERVF
GLIEDELTKLREKHCLPRRTEILDLGGGLEDIDLIANERSVVLLTETGYLKRMPVSEFEATSRGTRGKAGTRSQGEEAVK
LFISCNDHDTLLLFSDRGVSYALPAYRVPQCSRTAKGTPVVQLLPIPREEAITSLLSVISFDDDTHLLMLTKGGFIKRTS
LSAFSKIRSNGLIAIGLEEGDALTWVRLAVPGDSVLIASRAGMTIHFRLCDEELRPLGRTARGVRAMNLRGGDSLVSMDV
LPVELADQVAKSLEDDQDESGETVVVSDGPWVLVASASGLGKRVPVNQFRLQKRAGMGLRAIKFRREGDELVGLRVLGYG
EELLLVSERGVIVRTSADKIPQQSRAATGVRLQRLDAGDRLSEVVLVPPDSEDQDQPDDRSDPSEVAGVDDAPSS

Sequences:

>Translated_875_residues
MADPLGPSDGGPGESDDRIIQTDLRIEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMYELGLTSDRPYRKCARV
VGEVLGKYHPHGDTAVYDALVRMAQDFSMQMPLIDGHGNFGSVDNDPPAAMRYTESRLQSLTTDSLLEDIEAETVDYADN
FDGSQQEPMVLPARIPQLLLNGSSGIAVGMATNIPPHNLSELIDGLQALISNPELSDSELMTLIPGPDFPTGGQILGRSG
IRETYLTGRGSVTMRGVANIETIEHPGRADRDAVIITELPYQTNKAALIERIAEMVNDKKLEGISDIRDESDRDGMRIVV
ELRRDAYPQVVLNNLFKLTPLQSNFSAHMLALVNGEPVLLTLRKMLQVFLDFRVDTVERRTRYFLRKAEERDHILLGLLL
ALDQLDPIIALIRAASDTATARQQLQEHHGLTEIQADAILQMQLRRLTALEADKIRLEHEDLVTKIANYKDILGRRERVF
GLIEDELTKLREKHCLPRRTEILDLGGGLEDIDLIANERSVVLLTETGYLKRMPVSEFEATSRGTRGKAGTRSQGEEAVK
LFISCNDHDTLLLFSDRGVSYALPAYRVPQCSRTAKGTPVVQLLPIPREEAITSLLSVISFDDDTHLLMLTKGGFIKRTS
LSAFSKIRSNGLIAIGLEEGDALTWVRLAVPGDSVLIASRAGMTIHFRLCDEELRPLGRTARGVRAMNLRGGDSLVSMDV
LPVELADQVAKSLEDDQDESGETVVVSDGPWVLVASASGLGKRVPVNQFRLQKRAGMGLRAIKFRREGDELVGLRVLGYG
EELLLVSERGVIVRTSADKIPQQSRAATGVRLQRLDAGDRLSEVVLVPPDSEDQDQPDDRSDPSEVAGVDDAPSS
>Mature_874_residues
ADPLGPSDGGPGESDDRIIQTDLRIEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMYELGLTSDRPYRKCARVV
GEVLGKYHPHGDTAVYDALVRMAQDFSMQMPLIDGHGNFGSVDNDPPAAMRYTESRLQSLTTDSLLEDIEAETVDYADNF
DGSQQEPMVLPARIPQLLLNGSSGIAVGMATNIPPHNLSELIDGLQALISNPELSDSELMTLIPGPDFPTGGQILGRSGI
RETYLTGRGSVTMRGVANIETIEHPGRADRDAVIITELPYQTNKAALIERIAEMVNDKKLEGISDIRDESDRDGMRIVVE
LRRDAYPQVVLNNLFKLTPLQSNFSAHMLALVNGEPVLLTLRKMLQVFLDFRVDTVERRTRYFLRKAEERDHILLGLLLA
LDQLDPIIALIRAASDTATARQQLQEHHGLTEIQADAILQMQLRRLTALEADKIRLEHEDLVTKIANYKDILGRRERVFG
LIEDELTKLREKHCLPRRTEILDLGGGLEDIDLIANERSVVLLTETGYLKRMPVSEFEATSRGTRGKAGTRSQGEEAVKL
FISCNDHDTLLLFSDRGVSYALPAYRVPQCSRTAKGTPVVQLLPIPREEAITSLLSVISFDDDTHLLMLTKGGFIKRTSL
SAFSKIRSNGLIAIGLEEGDALTWVRLAVPGDSVLIASRAGMTIHFRLCDEELRPLGRTARGVRAMNLRGGDSLVSMDVL
PVELADQVAKSLEDDQDESGETVVVSDGPWVLVASASGLGKRVPVNQFRLQKRAGMGLRAIKFRREGDELVGLRVLGYGE
ELLLVSERGVIVRTSADKIPQQSRAATGVRLQRLDAGDRLSEVVLVPPDSEDQDQPDDRSDPSEVAGVDDAPSS

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=530, Percent_Identity=24.3396226415094, Blast_Score=90, Evalue=8e-18,
Organism=Homo sapiens, GI19913406, Length=371, Percent_Identity=25.8760107816712, Blast_Score=82, Evalue=3e-15,
Organism=Escherichia coli, GI1788562, Length=898, Percent_Identity=42.2048997772828, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=708, Percent_Identity=37.1468926553672, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=367, Percent_Identity=25.3405994550409, Blast_Score=76, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=287, Percent_Identity=27.8745644599303, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136538, Length=357, Percent_Identity=25.7703081232493, Blast_Score=75, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 96501; Mature: 96370

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPLGPSDGGPGESDDRIIQTDLRIEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRIL
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHH
YAMYELGLTSDRPYRKCARVVGEVLGKYHPHGDTAVYDALVRMAQDFSMQMPLIDGHGNF
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCC
GSVDNDPPAAMRYTESRLQSLTTDSLLEDIEAETVDYADNFDGSQQEPMVLPARIPQLLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHC
NGSSGIAVGMATNIPPHNLSELIDGLQALISNPELSDSELMTLIPGPDFPTGGQILGRSG
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHCCCC
IRETYLTGRGSVTMRGVANIETIEHPGRADRDAVIITELPYQTNKAALIERIAEMVNDKK
CCHHEECCCCCEEEEECCCCHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHH
LEGISDIRDESDRDGMRIVVELRRDAYPQVVLNNLFKLTPLQSNFSAHMLALVNGEPVLL
CCCHHHHCCCCCCCCCEEEEEEHHCCCHHHHHHHHHEECCCCCCCCEEEEEEECCCCHHH
TLRKMLQVFLDFRVDTVERRTRYFLRKAEERDHILLGLLLALDQLDPIIALIRAASDTAT
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARQQLQEHHGLTEIQADAILQMQLRRLTALEADKIRLEHEDLVTKIANYKDILGRRERVF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHEECHHHHHHHHHHHHHHHCCHHHHH
GLIEDELTKLREKHCLPRRTEILDLGGGLEDIDLIANERSVVLLTETGYLKRMPVSEFEA
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHEECCCEEEEEECCCCEECCCCHHHHH
TSRGTRGKAGTRSQGEEAVKLFISCNDHDTLLLFSDRGVSYALPAYRVPQCSRTAKGTPV
HCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCE
VQLLPIPREEAITSLLSVISFDDDTHLLMLTKGGFIKRTSLSAFSKIRSNGLIAIGLEEG
EEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHHCCCEEEEEECCC
DALTWVRLAVPGDSVLIASRAGMTIHFRLCDEELRPLGRTARGVRAMNLRGGDSLVSMDV
CEEEEEEEECCCCCEEEEECCCCEEEEEECCHHHHHHHHHHCCEEEEEECCCCCEEEEEE
LPVELADQVAKSLEDDQDESGETVVVSDGPWVLVASASGLGKRVPVNQFRLQKRAGMGLR
ECHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEE
AIKFRREGDELVGLRVLGYGEELLLVSERGVIVRTSADKIPQQSRAATGVRLQRLDAGDR
EEEEECCCCCEEEEEEECCCCEEEEEECCCEEEEECHHHCCCHHHHHCCCEEEECCCCCC
LSEVVLVPPDSEDQDQPDDRSDPSEVAGVDDAPSS
CCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
ADPLGPSDGGPGESDDRIIQTDLRIEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRIL
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHH
YAMYELGLTSDRPYRKCARVVGEVLGKYHPHGDTAVYDALVRMAQDFSMQMPLIDGHGNF
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCC
GSVDNDPPAAMRYTESRLQSLTTDSLLEDIEAETVDYADNFDGSQQEPMVLPARIPQLLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHC
NGSSGIAVGMATNIPPHNLSELIDGLQALISNPELSDSELMTLIPGPDFPTGGQILGRSG
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHCCCC
IRETYLTGRGSVTMRGVANIETIEHPGRADRDAVIITELPYQTNKAALIERIAEMVNDKK
CCHHEECCCCCEEEEECCCCHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHH
LEGISDIRDESDRDGMRIVVELRRDAYPQVVLNNLFKLTPLQSNFSAHMLALVNGEPVLL
CCCHHHHCCCCCCCCCEEEEEEHHCCCHHHHHHHHHEECCCCCCCCEEEEEEECCCCHHH
TLRKMLQVFLDFRVDTVERRTRYFLRKAEERDHILLGLLLALDQLDPIIALIRAASDTAT
HHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARQQLQEHHGLTEIQADAILQMQLRRLTALEADKIRLEHEDLVTKIANYKDILGRRERVF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHEECHHHHHHHHHHHHHHHCCHHHHH
GLIEDELTKLREKHCLPRRTEILDLGGGLEDIDLIANERSVVLLTETGYLKRMPVSEFEA
HHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHEECCCEEEEEECCCCEECCCCHHHHH
TSRGTRGKAGTRSQGEEAVKLFISCNDHDTLLLFSDRGVSYALPAYRVPQCSRTAKGTPV
HCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCE
VQLLPIPREEAITSLLSVISFDDDTHLLMLTKGGFIKRTSLSAFSKIRSNGLIAIGLEEG
EEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHHHCCCEEEEEECCC
DALTWVRLAVPGDSVLIASRAGMTIHFRLCDEELRPLGRTARGVRAMNLRGGDSLVSMDV
CEEEEEEEECCCCCEEEEECCCCEEEEEECCHHHHHHHHHHCCEEEEEECCCCCEEEEEE
LPVELADQVAKSLEDDQDESGETVVVSDGPWVLVASASGLGKRVPVNQFRLQKRAGMGLR
ECHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEE
AIKFRREGDELVGLRVLGYGEELLLVSERGVIVRTSADKIPQQSRAATGVRLQRLDAGDR
EEEEECCCCCEEEEEEECCCCEEEEEECCCEEEEECHHHCCCHHHHHCCCEEEECCCCCC
LSEVVLVPPDSEDQDQPDDRSDPSEVAGVDDAPSS
CCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]