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The map label for this gene is proP [H]

Identifier: 33863315

GI number: 33863315

Start: 1121086

End: 1122348

Strand: Reverse

Name: proP [H]

Synonym: PMT1044

Alternate gene names: 33863315

Gene position: 1122348-1121086 (Counterclockwise)

Preceding gene: 33863317

Following gene: 33863313

Centisome position: 46.55

GC content: 44.66

Gene sequence:

>1263_bases
ATGATCGCAAAAGCCCAAGAAACATCTAACATGCGTGTGATACTAGCGGGCCTCGTTGGTAACGTGATCGAGTGGTATGA
CTTTGCTTTGTATGGATACTTTGCCAACGTTATTGGACGACAGTTCTTTCCCTCAAGTAATCCTTCTGTTTCTCTAATTG
CTGCTTTCGGAGCGTTTGCCGTCGGCTTTCTAGTTCGGCCCTTTGGAGGACTTTTGTTCGGACGTATCGCTGATTTGCTG
GGACGAAAACAGGCTCTTATCCTTACCTTGATGGCTATGGCTATTCCAACAGTGCTAATGGCCTGTATGCCCAACTACAG
CAGGATTGGTGTAACCGCCCCGATCATAGTTGTTTTGTTGCGTATCATTCAAGGATTATCAGTTGGCGGCGAGTACACAA
CATCGATTGTCTATCTCGTTGAGAATGCCCCTGATCAACGACGAGGCTTCTTTGCTATTTGGGGGCTATGGGGTGCAGTA
TTAGGAATTCTGTTGGCTTCAGCAGTCGCAAGTTTTCTTGCGAATATTCTTGACCATCAACAGCTAGATATCTGGGGCTG
GAGAGTGCCTTTTGCTCTTGGTTCTCTTGTCGCATTAATAGGACTTTTAATTCGTCGTGGCCTTGTTAGCGATGTATCTA
CTGATGTATCCAGAGACCCAGTACAACAGGTTTTTGGAAAATACCGTATGCAGGTATTACGCTTGTTCTTGCTTAATATT
GGTGGTGGTGTTGGTTTCTATGCAGCTTTTGTGTATGTTGTGAGTTATATAAAAGAAATAGATATGGTGTCTGAACGCAT
AGCTTTGAATATAAATACAGTTTCTATGGCAATACTTTTACTACTTTATCCATTAACAGCTTGGCTTTCAGACCGTCTTG
GACGCAAGCCATTGTTGCTCGCTGGGGGTGGCATGTTGATGTTTGGGTCGATTCCACTGTTTGAATTGATTCACACCACT
GATCCTTTACGAATTTTCGCAGGGCAGCTTGGATTTGTGATTGCACTCGCAACTCTTTCGGGAGGATTAAATGTCGCGAA
TGTGGAGCTAATGCCTAAGGTGGTTCGCTGTACCGGCCTGGCCTTTGCCTACAACACTTCTATGGGAATTTTTGGTGGTA
CAACACCATTAATAGCGACCTGGTTAATTCAGGGAAGTGGAAATCCAATTAGCCCTGCTTACTGGGTAGCGGGCAGTGCT
TCGATCACTTTATTAACAAGTATCTTTTGGGTTAGAGAGACGAGACTTTCATGCCTGTCTTGA

Upstream 100 bases:

>100_bases
AGTCTTAATCACACCATTTATGTTTTGCCTTAGGTCAGAATTTCTTGCTTAATCATGAGGGGAGACTCGCCTGAAAAGAC
CATTCTTTTAGTTATTTGGC

Downstream 100 bases:

>100_bases
ACACAAAAAATATAAATCAAGCATTGCTATTCCCCAATCAAGGTTATTTACTAGCCCATCATTAACCTGGGGAGATACCC
TCAAATTGTTTGGTTTGTGT

Product: major facilitator superfamily proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MIAKAQETSNMRVILAGLVGNVIEWYDFALYGYFANVIGRQFFPSSNPSVSLIAAFGAFAVGFLVRPFGGLLFGRIADLL
GRKQALILTLMAMAIPTVLMACMPNYSRIGVTAPIIVVLLRIIQGLSVGGEYTTSIVYLVENAPDQRRGFFAIWGLWGAV
LGILLASAVASFLANILDHQQLDIWGWRVPFALGSLVALIGLLIRRGLVSDVSTDVSRDPVQQVFGKYRMQVLRLFLLNI
GGGVGFYAAFVYVVSYIKEIDMVSERIALNINTVSMAILLLLYPLTAWLSDRLGRKPLLLAGGGMLMFGSIPLFELIHTT
DPLRIFAGQLGFVIALATLSGGLNVANVELMPKVVRCTGLAFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWVAGSA
SITLLTSIFWVRETRLSCLS

Sequences:

>Translated_420_residues
MIAKAQETSNMRVILAGLVGNVIEWYDFALYGYFANVIGRQFFPSSNPSVSLIAAFGAFAVGFLVRPFGGLLFGRIADLL
GRKQALILTLMAMAIPTVLMACMPNYSRIGVTAPIIVVLLRIIQGLSVGGEYTTSIVYLVENAPDQRRGFFAIWGLWGAV
LGILLASAVASFLANILDHQQLDIWGWRVPFALGSLVALIGLLIRRGLVSDVSTDVSRDPVQQVFGKYRMQVLRLFLLNI
GGGVGFYAAFVYVVSYIKEIDMVSERIALNINTVSMAILLLLYPLTAWLSDRLGRKPLLLAGGGMLMFGSIPLFELIHTT
DPLRIFAGQLGFVIALATLSGGLNVANVELMPKVVRCTGLAFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWVAGSA
SITLLTSIFWVRETRLSCLS
>Mature_420_residues
MIAKAQETSNMRVILAGLVGNVIEWYDFALYGYFANVIGRQFFPSSNPSVSLIAAFGAFAVGFLVRPFGGLLFGRIADLL
GRKQALILTLMAMAIPTVLMACMPNYSRIGVTAPIIVVLLRIIQGLSVGGEYTTSIVYLVENAPDQRRGFFAIWGLWGAV
LGILLASAVASFLANILDHQQLDIWGWRVPFALGSLVALIGLLIRRGLVSDVSTDVSRDPVQQVFGKYRMQVLRLFLLNI
GGGVGFYAAFVYVVSYIKEIDMVSERIALNINTVSMAILLLLYPLTAWLSDRLGRKPLLLAGGGMLMFGSIPLFELIHTT
DPLRIFAGQLGFVIALATLSGGLNVANVELMPKVVRCTGLAFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWVAGSA
SITLLTSIFWVRETRLSCLS

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=33.6515513126492, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788942, Length=367, Percent_Identity=33.7874659400545, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1788292, Length=422, Percent_Identity=31.042654028436, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=28.5714285714286, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI87082231, Length=200, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45458; Mature: 45458

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKAQETSNMRVILAGLVGNVIEWYDFALYGYFANVIGRQFFPSSNPSVSLIAAFGAFA
CCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VGFLVRPFGGLLFGRIADLLGRKQALILTLMAMAIPTVLMACMPNYSRIGVTAPIIVVLL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RIIQGLSVGGEYTTSIVYLVENAPDQRRGFFAIWGLWGAVLGILLASAVASFLANILDHQ
HHHHCCCCCCCHHHEEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QLDIWGWRVPFALGSLVALIGLLIRRGLVSDVSTDVSRDPVQQVFGKYRMQVLRLFLLNI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GGGVGFYAAFVYVVSYIKEIDMVSERIALNINTVSMAILLLLYPLTAWLSDRLGRKPLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
AGGGMLMFGSIPLFELIHTTDPLRIFAGQLGFVIALATLSGGLNVANVELMPKVVRCTGL
ECCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
AFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWVAGSASITLLTSIFWVRETRLSCLS
HEEEECCCCCCCCCHHHHHHHHEECCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAKAQETSNMRVILAGLVGNVIEWYDFALYGYFANVIGRQFFPSSNPSVSLIAAFGAFA
CCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
VGFLVRPFGGLLFGRIADLLGRKQALILTLMAMAIPTVLMACMPNYSRIGVTAPIIVVLL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RIIQGLSVGGEYTTSIVYLVENAPDQRRGFFAIWGLWGAVLGILLASAVASFLANILDHQ
HHHHCCCCCCCHHHEEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QLDIWGWRVPFALGSLVALIGLLIRRGLVSDVSTDVSRDPVQQVFGKYRMQVLRLFLLNI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GGGVGFYAAFVYVVSYIKEIDMVSERIALNINTVSMAILLLLYPLTAWLSDRLGRKPLLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
AGGGMLMFGSIPLFELIHTTDPLRIFAGQLGFVIALATLSGGLNVANVELMPKVVRCTGL
ECCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
AFAYNTSMGIFGGTTPLIATWLIQGSGNPISPAYWVAGSASITLLTSIFWVRETRLSCLS
HEEEECCCCCCCCCHHHHHHHHEECCCCCCCCHHEECCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]