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The map label for this gene is gap3 [H]

Identifier: 33863271

GI number: 33863271

Start: 1078038

End: 1079093

Strand: Reverse

Name: gap3 [H]

Synonym: PMT1000

Alternate gene names: 33863271

Gene position: 1079093-1078038 (Counterclockwise)

Preceding gene: 33863276

Following gene: 33863270

Centisome position: 44.76

GC content: 44.51

Gene sequence:

>1056_bases
ATGGCCAAGCAAGGGCCAATGCGTATTGGTATCAACGGTTTTGGGCGTATCGGGCGGCTGGTCTTCAGGGCTCTCTGGGG
GAGACCAGGGATCGAGATCTGCCATTTGAACGACCCGTCTGGGGATGCAGCCACTGCAGCACACCTGCTTAAGTTCGATT
CTGTACACGGACGCTGGGATCAGCTTGTTGGAGGCAGCGAGAACCAACTGTTGGTGAATCAACAAACCATCAGTTACTCC
CAAGGAAGCACTCCTGGCGCTGTGCCTTGGAATGACGCTGGCATTGACATCGTTTTGGAATGCAGCGGTAAATTTAAATC
ACCAGAAACTCTCAGCGTTTACTTCGATCAACATGCTTTAAAACGTGTGATTGTTGCTTGCCCAGTTAAAGGAAAACTAG
CTGGCAAAGAAGTGTTGAATATCGTTTATGGGATTAATCATCATCTTTATGACCCAAAGATTGATCATTTAGTGACAGCA
GCATCATGTACGACAAACTGTTTGGCTCCGTTGGTAAAGGTTGTGCATGAAAACTTTGGCATTTCACATGGCTGCATAAC
AACTCTTCATGATACTACTAATACACAAGTTCCGATTGATAGTTTCCAAACGGATTTACGTAGGGCTAGGAGTTGTCTAC
AAAGTTTGATCCCAACAACGACTGGCTCTGCCAAAGCAATCGCAATGATCTTTCCAGATCTAGAGGGAAAACTCAATGGA
CATGCTGTAAGAGTACCTTTGCTCAATGCTTCATTGACCGATGCAGTTTTTGAGCTAAAGCGCTCAATTACGGTGAAGGA
TGCCAATGAAGCGTTTAGGCATGCAGCAGAACATCAGCTAAAGGGAATCCTTGGCTATGAAGACAAGCCACTTGTCTCTA
TTGATTACGTTAACGATTCTCGAAGCTCAATTGTCGATGGATTGTCAACGATGGTTGTTAACGGCTCTCAGCTAAAGGTT
TACGCATGGTACGACAATGAATGGGGATATAGCAGTCGAATGGCAGATCTTACGTGTTATATTTCTAATCTTGAAAATAC
TAGTAATACCTTTTAA

Upstream 100 bases:

>100_bases
TTAATGTAAGACGTTTTTTACTTTAAGGACAATATAAGAAAGGGAAAAGAGCGCTTGCATCGACTATGGTTGATACATCA
AGATATTTTGATTGATCCTT

Downstream 100 bases:

>100_bases
ATGATGCTTTCGCCTCTCAATCAATATGGTGTTGTAACCACAAACTACTGGGCCTTTACGCTCACTGATGGTGCATTACG
TATGCTTGTGGTTTTTCACT

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MAKQGPMRIGINGFGRIGRLVFRALWGRPGIEICHLNDPSGDAATAAHLLKFDSVHGRWDQLVGGSENQLLVNQQTISYS
QGSTPGAVPWNDAGIDIVLECSGKFKSPETLSVYFDQHALKRVIVACPVKGKLAGKEVLNIVYGINHHLYDPKIDHLVTA
ASCTTNCLAPLVKVVHENFGISHGCITTLHDTTNTQVPIDSFQTDLRRARSCLQSLIPTTTGSAKAIAMIFPDLEGKLNG
HAVRVPLLNASLTDAVFELKRSITVKDANEAFRHAAEHQLKGILGYEDKPLVSIDYVNDSRSSIVDGLSTMVVNGSQLKV
YAWYDNEWGYSSRMADLTCYISNLENTSNTF

Sequences:

>Translated_351_residues
MAKQGPMRIGINGFGRIGRLVFRALWGRPGIEICHLNDPSGDAATAAHLLKFDSVHGRWDQLVGGSENQLLVNQQTISYS
QGSTPGAVPWNDAGIDIVLECSGKFKSPETLSVYFDQHALKRVIVACPVKGKLAGKEVLNIVYGINHHLYDPKIDHLVTA
ASCTTNCLAPLVKVVHENFGISHGCITTLHDTTNTQVPIDSFQTDLRRARSCLQSLIPTTTGSAKAIAMIFPDLEGKLNG
HAVRVPLLNASLTDAVFELKRSITVKDANEAFRHAAEHQLKGILGYEDKPLVSIDYVNDSRSSIVDGLSTMVVNGSQLKV
YAWYDNEWGYSSRMADLTCYISNLENTSNTF
>Mature_350_residues
AKQGPMRIGINGFGRIGRLVFRALWGRPGIEICHLNDPSGDAATAAHLLKFDSVHGRWDQLVGGSENQLLVNQQTISYSQ
GSTPGAVPWNDAGIDIVLECSGKFKSPETLSVYFDQHALKRVIVACPVKGKLAGKEVLNIVYGINHHLYDPKIDHLVTAA
SCTTNCLAPLVKVVHENFGISHGCITTLHDTTNTQVPIDSFQTDLRRARSCLQSLIPTTTGSAKAIAMIFPDLEGKLNGH
AVRVPLLNASLTDAVFELKRSITVKDANEAFRHAAEHQLKGILGYEDKPLVSIDYVNDSRSSIVDGLSTMVVNGSQLKVY
AWYDNEWGYSSRMADLTCYISNLENTSNTF

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=342, Percent_Identity=38.8888888888889, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI7657116, Length=336, Percent_Identity=38.6904761904762, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1788079, Length=337, Percent_Identity=40.3560830860534, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789295, Length=342, Percent_Identity=36.5497076023392, Blast_Score=228, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI32566163, Length=340, Percent_Identity=38.8235294117647, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17568413, Length=340, Percent_Identity=38.8235294117647, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17534677, Length=340, Percent_Identity=38.8235294117647, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI17534679, Length=340, Percent_Identity=38.5294117647059, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321631, Length=338, Percent_Identity=38.4615384615385, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6322409, Length=338, Percent_Identity=38.4615384615385, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6322468, Length=337, Percent_Identity=38.2789317507418, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19922412, Length=333, Percent_Identity=39.3393393393393, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI17933600, Length=335, Percent_Identity=40, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI18110149, Length=335, Percent_Identity=40, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI85725000, Length=337, Percent_Identity=40.0593471810089, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI22023983, Length=337, Percent_Identity=40.0593471810089, Blast_Score=244, Evalue=7e-65,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 38365; Mature: 38234

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQGPMRIGINGFGRIGRLVFRALWGRPGIEICHLNDPSGDAATAAHLLKFDSVHGRWD
CCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHH
QLVGGSENQLLVNQQTISYSQGSTPGAVPWNDAGIDIVLECSGKFKSPETLSVYFDQHAL
HHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECHHHH
KRVIVACPVKGKLAGKEVLNIVYGINHHLYDPKIDHLVTAASCTTNCLAPLVKVVHENFG
HHHHEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHCC
ISHGCITTLHDTTNTQVPIDSFQTDLRRARSCLQSLIPTTTGSAKAIAMIFPDLEGKLNG
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCC
HAVRVPLLNASLTDAVFELKRSITVKDANEAFRHAAEHQLKGILGYEDKPLVSIDYVNDS
CEEEEEEECCHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
RSSIVDGLSTMVVNGSQLKVYAWYDNEWGYSSRMADLTCYISNLENTSNTF
HHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
AKQGPMRIGINGFGRIGRLVFRALWGRPGIEICHLNDPSGDAATAAHLLKFDSVHGRWD
CCCCCEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHH
QLVGGSENQLLVNQQTISYSQGSTPGAVPWNDAGIDIVLECSGKFKSPETLSVYFDQHAL
HHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECHHHH
KRVIVACPVKGKLAGKEVLNIVYGINHHLYDPKIDHLVTAASCTTNCLAPLVKVVHENFG
HHHHEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHCC
ISHGCITTLHDTTNTQVPIDSFQTDLRRARSCLQSLIPTTTGSAKAIAMIFPDLEGKLNG
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCC
HAVRVPLLNASLTDAVFELKRSITVKDANEAFRHAAEHQLKGILGYEDKPLVSIDYVNDS
CEEEEEEECCHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
RSSIVDGLSTMVVNGSQLKVYAWYDNEWGYSSRMADLTCYISNLENTSNTF
HHHHHHHHHHEEECCCEEEEEEEECCCCCCCCCHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8378350 [H]