Click here to switch to the map view.

The map label for this gene is ruvA

Identifier: 33862917

GI number: 33862917

Start: 697936

End: 698613

Strand: Direct

Name: ruvA

Synonym: PMT0644

Alternate gene names: 33862917

Gene position: 697936-698613 (Clockwise)

Preceding gene: 33862916

Following gene: 33862918

Centisome position: 28.95

GC content: 55.16

Gene sequence:

>678_bases
ATGATTGGCTGGCTGCAAGGGCAAAAGGTAGAAGCCTGGCAGCAAGGGACTCGGCAAGGAGTCGTTCTGGCTTGTGCTGG
TGTTGGTTACGAGGTTCAGATTGCACCGCGCCATCTCAGCGAAATGGAGCACGGGCAAAACACATTCATCCTCTGGATCC
ATCAGGTGCAGCGTGATGATGGATCCAGCTTGTTTGGCTTCCCGGAACGACGAGAACGGGACATGTTTCGTACCTTGATT
GGTGTCAGCGGCGTGGGGCCCCAGATGGCTCTCGCTCTTCTAGAGGAATGTCAGACCGGAGAACTTGTAGAGGCGATTGT
GCAGGGAGACCTACGCAAGCTTTGTCAGGCTCAAGGTGTAGGCAAGCGCACTGCCGAACGTCTTGCGGTGGAATTGCGCA
CAAAGTTGGCTGAATTTAGTTGTCGTGATCCTGGAATGTCCCTGGTGGACAATGGTGTGATCGATTCGCATCAGCTCAAG
GATTCAAGTCTGCATGAGCTACAGATCACCCTCGGGGGCCTTGGCTACGAAGATCTTGAAATACGCCGTGCCATAAGGGC
TGTGGCCAGCGGTGCTGCAATAGGAGCCAGTGACATGCCTGAGTCTGTCCCTTCCATCGACGACACTGACGCCTGGCTGA
GAGCAAGCCTGCGCTGGCTTAGTCAGGAAGCTGCCTAA

Upstream 100 bases:

>100_bases
CGCAAAGCAAAACAAGCTCCAGAAGCTCTAATCAGATCCTCGAACGATCAAGCAGAGGCTGGACAGATCAGGCAGCATGG
ATGACATCAAGAGCAACCTT

Downstream 100 bases:

>100_bases
ACAATGGTCCTCAAAGAGTTAGATTGTTTTTTTGGTCTCTCAGGGCAAAACGCCAAGCATGTCGCTCGATACAACAGAAA
AGCAACAGCTGATCAATGCC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDDGSSLFGFPERRERDMFRTLI
GVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNGVIDSHQLK
DSSLHELQITLGGLGYEDLEIRRAIRAVASGAAIGASDMPESVPSIDDTDAWLRASLRWLSQEAA

Sequences:

>Translated_225_residues
MIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDDGSSLFGFPERRERDMFRTLI
GVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNGVIDSHQLK
DSSLHELQITLGGLGYEDLEIRRAIRAVASGAAIGASDMPESVPSIDDTDAWLRASLRWLSQEAA
>Mature_225_residues
MIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDDGSSLFGFPERRERDMFRTLI
GVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGVGKRTAERLAVELRTKLAEFSCRDPGMSLVDNGVIDSHQLK
DSSLHELQITLGGLGYEDLEIRRAIRAVASGAAIGASDMPESVPSIDDTDAWLRASLRWLSQEAA

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=191, Percent_Identity=31.413612565445, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_PROMM (Q7V7T9)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894477.1
- ProteinModelPortal:   Q7V7T9
- SMR:   Q7V7T9
- STRING:   Q7V7T9
- GeneID:   1728975
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0644
- NMPDR:   fig|74547.1.peg.644
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   THLQVRE
- ProtClustDB:   PRK00116
- BioCyc:   PMAR74547:PMT0644-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =3.6.4.12

Molecular weight: Translated: 24695; Mature: 24695

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDD
CCCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHCCCCEEEEEEEEECCCC
GSSLFGFPERRERDMFRTLIGVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGV
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
GKRTAERLAVELRTKLAEFSCRDPGMSLVDNGVIDSHQLKDSSLHELQITLGGLGYEDLE
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHCCCCCCEEEEEEECCCCCHHHH
IRRAIRAVASGAAIGASDMPESVPSIDDTDAWLRASLRWLSQEAA
HHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIGWLQGQKVEAWQQGTRQGVVLACAGVGYEVQIAPRHLSEMEHGQNTFILWIHQVQRDD
CCCCCCCCCHHHHHCCCCCCEEEEEECCCEEEEECHHHHHHHHCCCCEEEEEEEEECCCC
GSSLFGFPERRERDMFRTLIGVSGVGPQMALALLEECQTGELVEAIVQGDLRKLCQAQGV
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
GKRTAERLAVELRTKLAEFSCRDPGMSLVDNGVIDSHQLKDSSLHELQITLGGLGYEDLE
CHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHCCCCCCEEEEEEECCCCCHHHH
IRRAIRAVASGAAIGASDMPESVPSIDDTDAWLRASLRWLSQEAA
HHHHHHHHHCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642