Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 33862827

GI number: 33862827

Start: 609012

End: 609926

Strand: Direct

Name: opuAB [H]

Synonym: PMT0554

Alternate gene names: 33862827

Gene position: 609012-609926 (Clockwise)

Preceding gene: 33862826

Following gene: 33862828

Centisome position: 25.26

GC content: 52.13

Gene sequence:

>915_bases
ATGATGGATGTGAACGACCAAGTTTTTGCAACCGTCAGTACCGGCCCTGTCGGCGAGGGCATGAGCAGATTCGTGGACTG
GTTGCTCAATCATGCGCAACCAATTTTCCTAGTAATTGATTCTGCAATTAATGGGTTAGCCGGAGCCATCGAACAGATCC
TCTCCGTACCAGCGCCCTGGCTGCTAGCTCCCCTTATCGCCATACTCGCAGCTTGGCGAGTGAGTTTTAGCTTCGCCATT
CTCAGCCTTCTTGGTCTGAACCTCGTTTTGTTCATGGGGCTTTGGCCGCCAATGATCTCAACCCTGGCCCTAGTTATTGC
AGCCTCATTACTGGCACTAATCATTGGCATCCCGATCGGCATTTTTTCTGCCCGTCGCCAACACATCTGGGCGATTACCC
GCCCAGTATTGGATCTGATGCAAACCATGCCGGCATTCGTTTATCTCATTCCGGCAGTGATGTTTTTCGGCACAGGGCTT
GTCCCATCCACTATCGCAACGCTGATCTTCTCGATGCCACCCGTAGTACGCCTGACGTACCTGGGCATACGACAAGTCCC
TGTTGATCTAATCGAAGCCGGGCGCGCCTTCGGTTGCTCAGAACGACAATTGCTCTGGAAAGTGCAACTTCCAAACGCCT
TACCAACCTTGATGGCTGGTGTTAATCAAACCATCATGCTCGCGCTATCGATGGTTGTGATCGCATCAATGATTGGTGGG
GGCGGCCTAGGTGATGTGGTGCTCAGAGGCATCCAACAACTTGATGTAGGCCTTGGCTTCGAGGGAGGGCTCGCCGTGGT
AATTCTGGCTGTAATCCTGGATCGACTCACACAAAGCCTGGCTGCCTCAAACTTTTCGAGAAAGTCATTGCCGCAGCGCT
TTAAAGCCTTTATTAACCTCTGGACTTCCTCATGA

Upstream 100 bases:

>100_bases
CTGTGGCCAACGCCCCGTATCCAGTTCCAGTGATTGGATCAGATCAGCGCCTCATTGGCGTGATGAGTCCACGTCGACTT
TTGCGCTCGATGATCCTGAG

Downstream 100 bases:

>100_bases
AAATGTCTAACAAGTTGCGAAATCTAAGCCTTGTTATCGCTGGAGTGCTGATGACTGGGTTGTCGGGATGTTCGAATGCC
AAACCAGATCAATTGCGCAT

Product: glycine betaine/proline ABC transporter

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MMDVNDQVFATVSTGPVGEGMSRFVDWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPWLLAPLIAILAAWRVSFSFAI
LSLLGLNLVLFMGLWPPMISTLALVIAASLLALIIGIPIGIFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGL
VPSTIATLIFSMPPVVRLTYLGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFINLWTSS

Sequences:

>Translated_304_residues
MMDVNDQVFATVSTGPVGEGMSRFVDWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPWLLAPLIAILAAWRVSFSFAI
LSLLGLNLVLFMGLWPPMISTLALVIAASLLALIIGIPIGIFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGL
VPSTIATLIFSMPPVVRLTYLGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFINLWTSS
>Mature_304_residues
MMDVNDQVFATVSTGPVGEGMSRFVDWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPWLLAPLIAILAAWRVSFSFAI
LSLLGLNLVLFMGLWPPMISTLALVIAASLLALIIGIPIGIFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGL
VPSTIATLIFSMPPVVRLTYLGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFINLWTSS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=276, Percent_Identity=50, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1788451, Length=200, Percent_Identity=34.5, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788449, Length=182, Percent_Identity=32.967032967033, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=29.6774193548387, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32658; Mature: 32658

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDVNDQVFATVSTGPVGEGMSRFVDWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPW
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHCCCHH
LLAPLIAILAAWRVSFSFAILSLLGLNLVLFMGLWPPMISTLALVIAASLLALIIGIPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGLVPSTIATLIFSMPPVVRLTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
LGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
HHHHHCCHHHHHCCCCCCCCCHHEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFINL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WTSS
HCCC
>Mature Secondary Structure
MMDVNDQVFATVSTGPVGEGMSRFVDWLLNHAQPIFLVIDSAINGLAGAIEQILSVPAPW
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHCCCHH
LLAPLIAILAAWRVSFSFAILSLLGLNLVLFMGLWPPMISTLALVIAASLLALIIGIPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
IFSARRQHIWAITRPVLDLMQTMPAFVYLIPAVMFFGTGLVPSTIATLIFSMPPVVRLTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
LGIRQVPVDLIEAGRAFGCSERQLLWKVQLPNALPTLMAGVNQTIMLALSMVVIASMIGG
HHHHHCCHHHHHCCCCCCCCCHHEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHCC
GGLGDVVLRGIQQLDVGLGFEGGLAVVILAVILDRLTQSLAASNFSRKSLPQRFKAFINL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
WTSS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]