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The map label for this gene is hofD [H]

Identifier: 33862806

GI number: 33862806

Start: 581543

End: 582355

Strand: Direct

Name: hofD [H]

Synonym: PMT0533

Alternate gene names: 33862806

Gene position: 581543-582355 (Clockwise)

Preceding gene: 33862805

Following gene: 33862807

Centisome position: 24.12

GC content: 55.23

Gene sequence:

>813_bases
ATGCTCTGGATCTATCTAGCGATCATCGGAGCTTGTATGGGCAGCTTCATCAATGTGATGGCTTGGCGGCTTCCACGAAA
GGAATCATTGATTTGGCCTGGTAGCCATTGCCCACGTTGTGGATGTGGTGTGCGTTGGCACGACAACATCCCAGTACTCG
CATGGATTCAACTGGGCGGTCGCTGTCGTGATTGCAGAACCACCATTCCAGTGCGTTATCCAGCGGTGGAGAGTCTCAGC
GCCGGGCTTTGGGTATCGGCTGCCTTGGCAGCTCCAACCAGCATGGGCGAGTTGTCTTCCTTGTTCAATCTCTTTGCTGG
AGTCGTGCTTGTCAGTGTGCTGTTGCCACTGGTCCTCATCGACCTCGATCACATGTGGTTGCCTGAACCACTTTGTCGGG
CTGGCCTTTTGTTAGGCCTGGCGTTCACCGCGACAGCGGCAGTTTCACTCGGCTGGAGTAAGGGATCAACCCTGCTACTC
GACCATTTACTAGCAGCAGCAGCAGCCTTAATTGCGATGGAAGGGCTTAGCTTCCTTGCCAAAAAGCTGATTGGTCAGCC
GGCCCTTGGGCTTGGTGATGCCAAATTGGCGGCCATGGCTGGAGCATGGTTAGGACTGAATGGGGTGGCTATGGCCATGA
CCCTGGCCATCATCAGCGGCGCAGCGGTTGGTCTCATCGGTCGGCTTGCTGGTCAGCTACAACCTCGCCAGCCTTTTCCC
TTCGGGCCTTTCATTGCCTTTGGGGTCTGGGGTGTATGGCTTTGTGGATCAGATTGGTGGTGGCAACATTGGCTCAACCT
AATGGGTCTTTAA

Upstream 100 bases:

>100_bases
TTCCAAAAGAAAAATTTCTCAAGCTAGAGCTCCTGCGCACCCTCCCGATGCGCTCCCGTCAAACCCGTGCAAGAACCATC
GCCATCTCTAGGCGCAGCTA

Downstream 100 bases:

>100_bases
TAGACCAACTGCTGCTAGAAAAAAATGCCAACTGTCAGTGAATCAAGCGCGCTTTAGATTTGGATGAATCACCACTTCTT
AATTTGCGGTATTGGACATA

Product: type 4 prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLWIYLAIIGACMGSFINVMAWRLPRKESLIWPGSHCPRCGCGVRWHDNIPVLAWIQLGGRCRDCRTTIPVRYPAVESLS
AGLWVSAALAAPTSMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLCRAGLLLGLAFTATAAVSLGWSKGSTLLL
DHLLAAAAALIAMEGLSFLAKKLIGQPALGLGDAKLAAMAGAWLGLNGVAMAMTLAIISGAAVGLIGRLAGQLQPRQPFP
FGPFIAFGVWGVWLCGSDWWWQHWLNLMGL

Sequences:

>Translated_270_residues
MLWIYLAIIGACMGSFINVMAWRLPRKESLIWPGSHCPRCGCGVRWHDNIPVLAWIQLGGRCRDCRTTIPVRYPAVESLS
AGLWVSAALAAPTSMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLCRAGLLLGLAFTATAAVSLGWSKGSTLLL
DHLLAAAAALIAMEGLSFLAKKLIGQPALGLGDAKLAAMAGAWLGLNGVAMAMTLAIISGAAVGLIGRLAGQLQPRQPFP
FGPFIAFGVWGVWLCGSDWWWQHWLNLMGL
>Mature_270_residues
MLWIYLAIIGACMGSFINVMAWRLPRKESLIWPGSHCPRCGCGVRWHDNIPVLAWIQLGGRCRDCRTTIPVRYPAVESLS
AGLWVSAALAAPTSMGELSSLFNLFAGVVLVSVLLPLVLIDLDHMWLPEPLCRAGLLLGLAFTATAAVSLGWSKGSTLLL
DHLLAAAAALIAMEGLSFLAKKLIGQPALGLGDAKLAAMAGAWLGLNGVAMAMTLAIISGAAVGLIGRLAGQLQPRQPFP
FGPFIAFGVWGVWLCGSDWWWQHWLNLMGL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=258, Percent_Identity=32.9457364341085, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 28865; Mature: 28865

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWIYLAIIGACMGSFINVMAWRLPRKESLIWPGSHCPRCGCGVRWHDNIPVLAWIQLGG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEECCCCCEEEEEECCC
RCRDCRTTIPVRYPAVESLSAGLWVSAALAAPTSMGELSSLFNLFAGVVLVSVLLPLVLI
CCCCCCCCCCEECCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DLDHMWLPEPLCRAGLLLGLAFTATAAVSLGWSKGSTLLLDHLLAAAAALIAMEGLSFLA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KKLIGQPALGLGDAKLAAMAGAWLGLNGVAMAMTLAIISGAAVGLIGRLAGQLQPRQPFP
HHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FGPFIAFGVWGVWLCGSDWWWQHWLNLMGL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLWIYLAIIGACMGSFINVMAWRLPRKESLIWPGSHCPRCGCGVRWHDNIPVLAWIQLGG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEECCCCCEEEEEECCC
RCRDCRTTIPVRYPAVESLSAGLWVSAALAAPTSMGELSSLFNLFAGVVLVSVLLPLVLI
CCCCCCCCCCEECCCHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DLDHMWLPEPLCRAGLLLGLAFTATAAVSLGWSKGSTLLLDHLLAAAAALIAMEGLSFLA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KKLIGQPALGLGDAKLAAMAGAWLGLNGVAMAMTLAIISGAAVGLIGRLAGQLQPRQPFP
HHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FGPFIAFGVWGVWLCGSDWWWQHWLNLMGL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]