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The map label for this gene is nth [H]

Identifier: 33862777

GI number: 33862777

Start: 556147

End: 556770

Strand: Direct

Name: nth [H]

Synonym: PMT0504

Alternate gene names: 33862777

Gene position: 556147-556770 (Clockwise)

Preceding gene: 33862776

Following gene: 33862779

Centisome position: 23.07

GC content: 43.11

Gene sequence:

>624_bases
ATGCGAAGACTAAACGAGCACTATCCTGATCCTGCCATTCCGCTCAACCATCATGACGATTTCACCCTATTGATTGCAGT
TGTTTTAAGTGCGCAGTGCACTGATAAGAAAGTCAATGAGGTGACAGTATCTCTGTTTGAACATGCACAGACCCCAGAGG
AGATGTATCAGTTAGGTGAAGTGAGAATACTAAATATGATCCGACAGCTAGGGCTGTCAAAGCAAAAAGCCAAAAACATA
CATAGACTCTCGGAAATCATTGTTCAACGCTTCCATTCTTCCGTACCACAGAATTTCAATGATCTAGAATCACTCCCTGG
AGTGGGACATAAAACAGCAAGTGTTGTCATGGCTCAAGCTTTTGGGGTGCCTACATTTCCAGTTGATACGCATATCCACC
GTTTAGCTCAAAGATGGGGACTATCTAATGGCAGCAGTGTACTTCAGACAGAAAAAGATCTCAAAAAGCTGTTCCCGAAA
TCAGCATGGAATAAGCTTCATCTCCAGATCATCTACTATGGTCGTGAAAACTGTACCGCAAGGGGATGTGATGGAACAAC
CTGTGATTTATGTAGAGAGCTATATCCAAAACGACGCAATCCAGTAAAGTGGATCAAAGCCTAG

Upstream 100 bases:

>100_bases
AAGAAATTTTTTGACCAATGAATGATAAAAATGAAACAAGCACCTCAATTCGCAATTCAATGAAAGACACGTGAGAAAAA
ATCAACGAGCAGAAACGATC

Downstream 100 bases:

>100_bases
CCTGGTAAATAATAAGATATTGTCTGATAACTATGCATTCGCAAATGACGTCATGAGTTAAATATGAACGTATATATAGC
TACAAAAATATTTTGTTATT

Product: endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MRRLNEHYPDPAIPLNHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGEVRILNMIRQLGLSKQKAKNI
HRLSEIIVQRFHSSVPQNFNDLESLPGVGHKTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVLQTEKDLKKLFPK
SAWNKLHLQIIYYGRENCTARGCDGTTCDLCRELYPKRRNPVKWIKA

Sequences:

>Translated_207_residues
MRRLNEHYPDPAIPLNHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGEVRILNMIRQLGLSKQKAKNI
HRLSEIIVQRFHSSVPQNFNDLESLPGVGHKTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVLQTEKDLKKLFPK
SAWNKLHLQIIYYGRENCTARGCDGTTCDLCRELYPKRRNPVKWIKA
>Mature_207_residues
MRRLNEHYPDPAIPLNHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGEVRILNMIRQLGLSKQKAKNI
HRLSEIIVQRFHSSVPQNFNDLESLPGVGHKTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVLQTEKDLKKLFPK
SAWNKLHLQIIYYGRENCTARGCDGTTCDLCRELYPKRRNPVKWIKA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=161, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787920, Length=194, Percent_Identity=41.7525773195876, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17554540, Length=189, Percent_Identity=30.6878306878307, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=182, Percent_Identity=28.021978021978, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=168, Percent_Identity=27.3809523809524, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=30.5084745762712, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23651; Mature: 23651

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLNEHYPDPAIPLNHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGE
CCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
VRILNMIRQLGLSKQKAKNIHRLSEIIVQRFHSSVPQNFNDLESLPGVGHKTASVVMAQA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHH
FGVPTFPVDTHIHRLAQRWGLSNGSSVLQTEKDLKKLFPKSAWNKLHLQIIYYGRENCTA
HCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHCCCEEEEEEEEECCCCCCC
RGCDGTTCDLCRELYPKRRNPVKWIKA
CCCCCCHHHHHHHHCCCCCCCCHHCCC
>Mature Secondary Structure
MRRLNEHYPDPAIPLNHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGE
CCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHH
VRILNMIRQLGLSKQKAKNIHRLSEIIVQRFHSSVPQNFNDLESLPGVGHKTASVVMAQA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHH
FGVPTFPVDTHIHRLAQRWGLSNGSSVLQTEKDLKKLFPKSAWNKLHLQIIYYGRENCTA
HCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHCCCEEEEEEEEECCCCCCC
RGCDGTTCDLCRELYPKRRNPVKWIKA
CCCCCCHHHHHHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]