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The map label for this gene is rne [H]

Identifier: 308535182

GI number: 308535182

Start: 828110

End: 830869

Strand: Direct

Name: rne [H]

Synonym: NA

Alternate gene names: 308535182

Gene position: 828110-830869 (Clockwise)

Preceding gene: 197117092

Following gene: 197117097

Centisome position: 17.94

GC content: 65.51

Gene sequence:

>2760_bases
ATGTCAAAAAAAATGCTGATTAATGCGCTCCACCCAGAGGAAGCGCGAGTGGCGATCGTCGAGGACGGCCGCCTGGTTGA
TCTGGATGTAGAGATCGCCGGCAACGAGCAGATCCGCGGCAACATCTACAAGGGGGTCGTGGTCCGAGTGGAGCAGGGGC
TGCAGGCCGCCTTCGTAGACATCGGGCTGAAGAAGCTCGGCTTCCTGCAGATGGGCGAACTGCATCCGGAAAACTGGAAG
TGGCGCGACGACATCCCCGAGGACCAGAGGCACCGCCGCCCGCGCATCCAGGAAGTGCTGCGCAGGGGGCAGGAGCTGAT
CGTCCAGGTGGAGAAGGGGGAGCGGGACAACAAGGGCTCCGCGCTCACCACCTACGTCTCTTTGCCTGGGCGCTACATGG
TGCTGATGCCCGGGAGCGACTCGGCCGGCATCTCCCGGAAAGTCGAGGGGGAAGGAGAGCGCAAGAAGCTCAAGGAAATC
ATCGCCGAGATGGCCATTCCCGAGGGGTACGGCTACATCATCCGGACCGAGGCGATGGGGAGGACCCGCGAAGAGCTCCA
GAAGGACCTCGACAACCTGATTGCGACCTACCAGGGGATCCGCTCGAAAGGTCTGGCCATGAAGGGAGCGGGGATGATCT
ACCAGGAGTCCGCGCTCATCATCAGGACCATCCGGGACTACTTCTCCGCCGACATCGACGAGGTTCTGGTGGACAGCAAG
GACGTCTACAACGATGTCCGCGACTGCTTGAAGGAGATCGACCCGGCCTTCGAAAAGCTGGTCAAGATGCACCAGGAGAA
GCGCCCCATCTTCTCGCGCTACCAGTTGGAGGAGCAGATCGACCTGATTTACGAGAAGAAGGTCCCCCTGAAGTCGGGCG
GGTCCATCTTCATCGAGCCGACCGAGGCACTGGTCTCGATCGACGTCAACTCGGGCAAGTCCACCGGCGAGAAGGGGGTC
GAGGATACCGCGTTCAAGACGAACCTGGAAGCGGCCGAGGAAGCTGCCCGGCAACTGCGGCTGCGCGACCTGGGCGGGCT
CATCGTCATCGATTTCATCGACATGCGCGACCGCAAGCACAACTCCGAAGTGGAAAAAACGCTGAAGACGGCGCTCAAGG
CCGACAAGGCGCGGGTGAACGTCTCGCGCATCTCGGAATTCGGCCTTTTGGAGATGTCGCGCCAGCGCATCCGCCAGACC
CTGAACCAGGCCTCCACCCTGGAATGTCCGCACTGCGACGGCAGGGGGAAGGTGAAATCGGTCGAGGCGATGGCGCTCTC
GTTCCTGCGGAAGGTGCACGCCGCCGCGGCCAAGGGGACCATCGGCGAGGTGGTGGGTGGCCTGCCGCTGGAGGTGGCCT
ACTACCTCTTGAACCGCAAACGCCACGAGCTTTCCCAGATCGAGAGCGACTACGACATCGAGGTGACGGTGAAAGGAAAA
CCGTCTTACCTCCTGAACCAGATGGATCTGGAACTGATCAAGCGGGAGAAGGCCGCAGACGAGCTGCCGCTGGAGAAGCC
GGCCCATGGGCGTGCGCTGCCTAAGGAAGAAGCGGCCCAGGAGCCCGCAGCCGAAGGGCGCAAGAAAAAGAAGAAGGGGA
AAAAGGCCCAGGAGCCGGAGGCCGCAGCTCACGTCGAGGCGCTGCCGGTTGCCGTCGTGGAGGAGCCGACCGGCGCCGAG
CTGGTGGTGACCGTCGAGGGCGAACCCGAGGAGCACAAGAAAAAGCGCCGCCGCAAGCGCAAGCGGGGCAAGGGAGGCGC
AGCCGAAGCGGGTGCCGAGGCCGCTGCGTCGCAATCGGTCGAGGCAGAAGTGGAGCTTCCGGCCGCAGAGCCAGGGACCC
TTCCCGCGCCGGGACCGGTCACGGAGCAGGCGGCTGCCGCCGAGGGTGAGCAGCTCCAGCACGAGGCGGGCGAAGAAGTG
AAGAAGAAGCGCCGGCGCAAAAGGCGGCGCGGCAAGGGAGGGCACGAGGCGCAGTTGCATGAGTCCGCAGCCGAGTTCCC
GCCGCAGTTGGAAGCTTTCGCGGCAGCGGGTGCCATCCCGGTGCAGGAAGTGACCCCGGTGATAACGGCCTCCGCCGTTT
CCGCCGAACCTGGCGCAGCAGCGAAGAAGGGGCGCAAAAGGGGTAAGGCGCGTGCCGAGGAGCCTGCTGTTTCGGCGGGC
GCGGCTCAGGTCGAGGCGATAACCGTTCCGGCAGAGGTCATCGAGTCGGCGCAACCCGCCGCGAAGCCGAAGCGTCCGCG
CGGCGGCAGGAAGGTGAAAGAAGCCGCCGCAGAGCAGGGCGCGCCGGCTGTGGTCGCAGCGGCGCCCGGTGGAGCACCTG
AGGCACCCGCCGGAGCCGCGGCCCCCGCGGAGCCCGCGAAGGCCAAAAGGAAACCGAGGGGAGCTAAGGCCGCCAAGGGC
GCTGAAGCGACCCCTGTCCCGGAAGCCGGCGAGGCAAAGGCCGCGCCGGCCGGAAAGAAAGCCCCCGCCGCGGTGACCGA
AGGAACGGAGGCTCCCGCCAAGGCGAAGGGAAAGAGCACCAAGGGTAAGAGCGCCGAGGCTCCCAAGGCGAAAAAGGAGC
AGGAAGCTGCCACCGCAGCGCCTGAACCGCCCCCCGCACCGGCTCAGGATAAAGCGCCTGCCAAGGCTCGCGCACCCCGT
GCCTCCCGTAAGAAAGAGGCACCGGAGGGCACCGGGCAGGCACCGGCCGCGCCGGTCGAGGAGAAGCCCAAGAAGCCGCG
GGCTCCCCGCAAGAAAAAAGAAGAAGCGCCCGCAGAATAG

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: ribonuclease, Rne/Rng family

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 919; Mature: 918

Protein sequence:
NA

Sequences:

>Translated_919_residues
MSKKMLINALHPEEARVAIVEDGRLVDLDVEIAGNEQIRGNIYKGVVVRVEQGLQAAFVDIGLKKLGFLQMGELHPENWK
WRDDIPEDQRHRRPRIQEVLRRGQELIVQVEKGERDNKGSALTTYVSLPGRYMVLMPGSDSAGISRKVEGEGERKKLKEI
IAEMAIPEGYGYIIRTEAMGRTREELQKDLDNLIATYQGIRSKGLAMKGAGMIYQESALIIRTIRDYFSADIDEVLVDSK
DVYNDVRDCLKEIDPAFEKLVKMHQEKRPIFSRYQLEEQIDLIYEKKVPLKSGGSIFIEPTEALVSIDVNSGKSTGEKGV
EDTAFKTNLEAAEEAARQLRLRDLGGLIVIDFIDMRDRKHNSEVEKTLKTALKADKARVNVSRISEFGLLEMSRQRIRQT
LNQASTLECPHCDGRGKVKSVEAMALSFLRKVHAAAAKGTIGEVVGGLPLEVAYYLLNRKRHELSQIESDYDIEVTVKGK
PSYLLNQMDLELIKREKAADELPLEKPAHGRALPKEEAAQEPAAEGRKKKKKGKKAQEPEAAAHVEALPVAVVEEPTGAE
LVVTVEGEPEEHKKKRRRKRKRGKGGAAEAGAEAAASQSVEAEVELPAAEPGTLPAPGPVTEQAAAAEGEQLQHEAGEEV
KKKRRRKRRRGKGGHEAQLHESAAEFPPQLEAFAAAGAIPVQEVTPVITASAVSAEPGAAAKKGRKRGKARAEEPAVSAG
AAQVEAITVPAEVIESAQPAAKPKRPRGGRKVKEAAAEQGAPAVVAAAPGGAPEAPAGAAAPAEPAKAKRKPRGAKAAKG
AEATPVPEAGEAKAAPAGKKAPAAVTEGTEAPAKAKGKSTKGKSAEAPKAKKEQEAATAAPEPPPAPAQDKAPAKARAPR
ASRKKEAPEGTGQAPAAPVEEKPKKPRAPRKKKEEAPAE
>Mature_918_residues
SKKMLINALHPEEARVAIVEDGRLVDLDVEIAGNEQIRGNIYKGVVVRVEQGLQAAFVDIGLKKLGFLQMGELHPENWKW
RDDIPEDQRHRRPRIQEVLRRGQELIVQVEKGERDNKGSALTTYVSLPGRYMVLMPGSDSAGISRKVEGEGERKKLKEII
AEMAIPEGYGYIIRTEAMGRTREELQKDLDNLIATYQGIRSKGLAMKGAGMIYQESALIIRTIRDYFSADIDEVLVDSKD
VYNDVRDCLKEIDPAFEKLVKMHQEKRPIFSRYQLEEQIDLIYEKKVPLKSGGSIFIEPTEALVSIDVNSGKSTGEKGVE
DTAFKTNLEAAEEAARQLRLRDLGGLIVIDFIDMRDRKHNSEVEKTLKTALKADKARVNVSRISEFGLLEMSRQRIRQTL
NQASTLECPHCDGRGKVKSVEAMALSFLRKVHAAAAKGTIGEVVGGLPLEVAYYLLNRKRHELSQIESDYDIEVTVKGKP
SYLLNQMDLELIKREKAADELPLEKPAHGRALPKEEAAQEPAAEGRKKKKKGKKAQEPEAAAHVEALPVAVVEEPTGAEL
VVTVEGEPEEHKKKRRRKRKRGKGGAAEAGAEAAASQSVEAEVELPAAEPGTLPAPGPVTEQAAAAEGEQLQHEAGEEVK
KKRRRKRRRGKGGHEAQLHESAAEFPPQLEAFAAAGAIPVQEVTPVITASAVSAEPGAAAKKGRKRGKARAEEPAVSAGA
AQVEAITVPAEVIESAQPAAKPKRPRGGRKVKEAAAEQGAPAVVAAAPGGAPEAPAGAAAPAEPAKAKRKPRGAKAAKGA
EATPVPEAGEAKAAPAGKKAPAAVTEGTEAPAKAKGKSTKGKSAEAPKAKKEQEAATAAPEPPPAPAQDKAPAKARAPRA
SRKKEAPEGTGQAPAAPVEEKPKKPRAPRKKKEEAPAE

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=473, Percent_Identity=46.9344608879493, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI87082235, Length=497, Percent_Identity=37.625754527163, Blast_Score=289, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 99041; Mature: 98910

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKMLINALHPEEARVAIVEDGRLVDLDVEIAGNEQIRGNIYKGVVVRVEQGLQAAFVD
CCCCCEEECCCCCCCEEEEEECCEEEEEEEEECCCCCCCCCHHHHHHEEHHHCHHHHHHH
IGLKKLGFLQMGELHPENWKWRDDIPEDQRHRRPRIQEVLRRGQELIVQVEKGERDNKGS
HHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHEEEEECCCCCCCCCC
ALTTYVSLPGRYMVLMPGSDSAGISRKVEGEGERKKLKEIIAEMAIPEGYGYIIRTEAMG
EEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHC
RTREELQKDLDNLIATYQGIRSKGLAMKGAGMIYQESALIIRTIRDYFSADIDEVLVDSK
CHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECHHHHHHHHHHHHHCCHHHHHHCCH
DVYNDVRDCLKEIDPAFEKLVKMHQEKRPIFSRYQLEEQIDLIYEKKVPLKSGGSIFIEP
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
TEALVSIDVNSGKSTGEKGVEDTAFKTNLEAAEEAARQLRLRDLGGLIVIDFIDMRDRKH
CCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCC
NSEVEKTLKTALKADKARVNVSRISEFGLLEMSRQRIRQTLNQASTLECPHCDGRGKVKS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
VEAMALSFLRKVHAAAAKGTIGEVVGGLPLEVAYYLLNRKRHELSQIESDYDIEVTVKGK
HHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PSYLLNQMDLELIKREKAADELPLEKPAHGRALPKEEAAQEPAAEGRKKKKKGKKAQEPE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCH
AAAHVEALPVAVVEEPTGAELVVTVEGEPEEHKKKRRRKRKRGKGGAAEAGAEAAASQSV
HHHHHHHCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHCHHHHHHCCC
EAEVELPAAEPGTLPAPGPVTEQAAAAEGEQLQHEAGEEVKKKRRRKRRRGKGGHEAQLH
CEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ESAAEFPPQLEAFAAAGAIPVQEVTPVITASAVSAEPGAAAKKGRKRGKARAEEPAVSAG
HHHHHCCCHHHHHHHCCCCCHHHHCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCC
AAQVEAITVPAEVIESAQPAAKPKRPRGGRKVKEAAAEQGAPAVVAAAPGGAPEAPAGAA
CCEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
APAEPAKAKRKPRGAKAAKGAEATPVPEAGEAKAAPAGKKAPAAVTEGTEAPAKAKGKST
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
KGKSAEAPKAKKEQEAATAAPEPPPAPAQDKAPAKARAPRASRKKEAPEGTGQAPAAPVE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCCCCCCCCCC
EKPKKPRAPRKKKEEAPAE
CCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
SKKMLINALHPEEARVAIVEDGRLVDLDVEIAGNEQIRGNIYKGVVVRVEQGLQAAFVD
CCCCEEECCCCCCCEEEEEECCEEEEEEEEECCCCCCCCCHHHHHHEEHHHCHHHHHHH
IGLKKLGFLQMGELHPENWKWRDDIPEDQRHRRPRIQEVLRRGQELIVQVEKGERDNKGS
HHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHCCHHEEEEECCCCCCCCCC
ALTTYVSLPGRYMVLMPGSDSAGISRKVEGEGERKKLKEIIAEMAIPEGYGYIIRTEAMG
EEEEEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHC
RTREELQKDLDNLIATYQGIRSKGLAMKGAGMIYQESALIIRTIRDYFSADIDEVLVDSK
CHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECHHHHHHHHHHHHHCCHHHHHHCCH
DVYNDVRDCLKEIDPAFEKLVKMHQEKRPIFSRYQLEEQIDLIYEKKVPLKSGGSIFIEP
HHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
TEALVSIDVNSGKSTGEKGVEDTAFKTNLEAAEEAARQLRLRDLGGLIVIDFIDMRDRKH
CCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCC
NSEVEKTLKTALKADKARVNVSRISEFGLLEMSRQRIRQTLNQASTLECPHCDGRGKVKS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
VEAMALSFLRKVHAAAAKGTIGEVVGGLPLEVAYYLLNRKRHELSQIESDYDIEVTVKGK
HHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PSYLLNQMDLELIKREKAADELPLEKPAHGRALPKEEAAQEPAAEGRKKKKKGKKAQEPE
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCH
AAAHVEALPVAVVEEPTGAELVVTVEGEPEEHKKKRRRKRKRGKGGAAEAGAEAAASQSV
HHHHHHHCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHCHHHHHHCCC
EAEVELPAAEPGTLPAPGPVTEQAAAAEGEQLQHEAGEEVKKKRRRKRRRGKGGHEAQLH
CEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ESAAEFPPQLEAFAAAGAIPVQEVTPVITASAVSAEPGAAAKKGRKRGKARAEEPAVSAG
HHHHHCCCHHHHHHHCCCCCHHHHCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCC
AAQVEAITVPAEVIESAQPAAKPKRPRGGRKVKEAAAEQGAPAVVAAAPGGAPEAPAGAA
CCEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCC
APAEPAKAKRKPRGAKAAKGAEATPVPEAGEAKAAPAGKKAPAAVTEGTEAPAKAKGKST
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCC
KGKSAEAPKAKKEQEAATAAPEPPPAPAQDKAPAKARAPRASRKKEAPEGTGQAPAAPVE
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCCCCCCCCCC
EKPKKPRAPRKKKEEAPAE
CCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]