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The map label for this gene is nifA [H]

Identifier: 308513322

GI number: 308513322

Start: 2712307

End: 2715018

Strand: Direct

Name: nifA [H]

Synonym: NA

Alternate gene names: 308513322

Gene position: 2712307-2715018 (Clockwise)

Preceding gene: 39997565

Following gene: 39997571

Centisome position: 71.11

GC content: 64.6

Gene sequence:

>2712_bases
GTGCGTCTCGTCTCCGGCCAGGACCGGATCCGCATCTACCTGGAAGACCTGACCCGCGGCGTCCTTACCTGTGTCCACGC
CTCGGGCCCCCTGGCCGATGAGATCCGCGAGGTGAGCTTCCCCATCATCTCCCGTGAGGCGTCGGTTTCCAGCGTCTTTG
TCTCCCAGTACGCCACCGAATTCCACTACGAGCCGGAGGGAAAACACACCTTCGACCGGGGCTTTGCCGAACGCTTCGCC
ATCGGCCACAGCTACATCCTGCCGGTGGTGAGCCAGGGGAAATCCATCGGCGTGGTCTGCGTCGACCGCTTCCGGCCGGG
GGAGATCCTGCGCGGCAAGGGGAAGGCGCTGCTGGGCGAGTTCGTCACCTCCGTGGCCGACCGGCTCGACGTCGCCCGCA
TCTACCACCAGCAGCTCCTGCTCGCCCGCCGGGTCGAGGAGTACAAGAAACGGGAAGCTGCCTCGTTCATGGTGCAGTCG
GCCGTGCGCCTCATCGACCGGCTGGTCCTCGCCTCGGTGCTGGTGCCCGTGCCCGGTCCGGAAGGCTCGTCGCGCCTCGC
CATCCTGGCCAGCCACTCGGAAGACCCCAGCCTGAAAAAGCAGTATGACGAGCAGGGAGAGATCGCTCTCCAGAGGGGTA
CCTCCCTCATCTCCCGGTTCCTGGACGACAACGCGGTCATCGCCGACGAACGGCTTCTGCGCCCGCTGTTCATTCCGGAC
CTGACCCAGCAGGAGCTCCAGAAAAAGGCCCTCACCGAGAAGATGGCGCTGCGCTCCCTCTACGTGGTGCCCCGCTACGA
GCCGTCCAGCCGCAAGGTCATCTGCCTGGTCAACTATTTCACCAAAGACCTGTACCGCTTCTCCGACTTCGAGATGGGCC
TGCTCCAGACCCATGCGGAGATGGCGGAGCGGATGGTGAACGAGATCGGCGGCGAACACTTGGAAATCCGCGTCCTGGCC
GAGATCACCGAACTCCTCCAGGAGCGCAACGAAGAGCTTTCCCCGTTCCTCACCCGGGTCCTGTCAATGGCCACGGAGCT
GATCGGCGCCGATACCGGCAGCATCGCCATCGTCCAGGAGCGTGACGGCGAAAAATGGCTTGTGGTGGAGGACGAAGAAG
GGACCATCGTCGGGGCCAAGAACAAGTCGTGGCTCAAGAAGTACATCCCCCCCTTCAGAATCGGCGGCCACGAGCTCCCC
GCCGAGGAGCGGAGCCTCACCGGCTACGTGGCCTGGTCCAAGCAGCCGAAGATCATCGCCCACGTGGCGGACGAGCAGGG
GGGCGAGGGGTTCCACCGCTCCATGCACGAGCTGATCAAGAGCGAGATCGCGGTCCCCATCGTCTGCGACGACGAGGTGA
TCGCCGTGGTCTGCCTCAACTCGCTCAAGCCCGCCTGGTTCACGGAAGAGCACAAGCGGATCCTGCAGATCATCGACCGG
CTCACCTCCCGGCACATCTCCGACGTCCAGCGGATCGAGCGGCTCGAGGGGGAGGTGACCCGGCTCAAGACTGACGTGGC
CTACAAAGACCCCCAGATCTCCTCCTACCGGCTCGGCAACATCATCGGCAACAGCCGCAAAGCCCAGGAGATCGTTGATT
TCATCAACACCGTGTCGGTGCCCCTCTTCAACCGGATCACCCTCTGGAGCAAGAACGTCCTCCAGGAGGCGACCATCGGC
CTCCCCTCCATCCTCGTCCAGGGGCAGACCGGCGCCGGCAAGGAGTTCTTCTTCAACAACCTCTACAACAAGCTGAACGA
GATGTACCGGGAGAAGCTCAACCCCGCTGGCCAGCTCCCCGTGAAAAAGACCAACATCGCGGCCTACAGCGGTGACCTGA
CCTACTCGGAACTCTTCGGCCACAAGAAGGGGGCCTTCACCGGCGCCTACAGCGACCGCAAGGGCATCCTGGAGGATGCC
GCCGGCGGGATCGTCTTCCTGGACGAGATCGGCGACGCCGACCCCAAGACCCAGGTGCAGCTGCTCCGGTTCCTGGACAA
CGGCGGGTTTGTGCGGCTGGGGGAAAACCAGGACCGTTTCAGCCGGGTGCTCCTGGTGGCCGCCACCAACAAGGATCTGG
CGGAAGAGATCCGCAAGGGGAACTTCCGGGAAGACCTCTACCACCGGCTGTCGGAGCTGGCGGTGCAGGTGCCGTCCCTG
AACGAGCGGCGCGAGGACATCCCCGACCTGGCCACCCACTTCCTGGGCAAGCTCTACCGCACCTACCGGGGGGACGAGTC
CAAGGACGCCGCCCCCACCCTGGCGGAGGAGGCCAAGCGGCTCCTGATGAACCACCACTACCACGGCAACATCAGGGAAC
TGCGGAGCATCCTGCTGCGGGCGCTCTTCTTCCGCAAGGGCACGGTGCTCACCGCCGACGACGTCCGGCGCGCCCTGGCC
GCGGGCATGCGCGAGTTCGCCCCTGCCACCGCCACCCAGGAGCTGAACGACCGGATGGTGACGGAAATCCTGGACAAGAT
CGCCAATGGCGAAACCTTCTGGGAAGCGGTCTACGAGCCCTACTCCCGCAACGCCATCCCCCGCGACGCCGTCCGGCTCG
TCATCGAGCGGAGCCGCGACGCGGCGGGCCGGAGCATGCCCCAGGTGGCCCGCTACCTCAAGGCCGTGGGCGACGATGTG
GAAGAAAACGACGAGGAGCGGAAGCGGTTCTTCAAGTTCAAGAATTTCCTCTATAAGACCGTGAAGATCTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 903; Mature: 903

Protein sequence:
NA

Sequences:

>Translated_903_residues
MRLVSGQDRIRIYLEDLTRGVLTCVHASGPLADEIREVSFPIISREASVSSVFVSQYATEFHYEPEGKHTFDRGFAERFA
IGHSYILPVVSQGKSIGVVCVDRFRPGEILRGKGKALLGEFVTSVADRLDVARIYHQQLLLARRVEEYKKREAASFMVQS
AVRLIDRLVLASVLVPVPGPEGSSRLAILASHSEDPSLKKQYDEQGEIALQRGTSLISRFLDDNAVIADERLLRPLFIPD
LTQQELQKKALTEKMALRSLYVVPRYEPSSRKVICLVNYFTKDLYRFSDFEMGLLQTHAEMAERMVNEIGGEHLEIRVLA
EITELLQERNEELSPFLTRVLSMATELIGADTGSIAIVQERDGEKWLVVEDEEGTIVGAKNKSWLKKYIPPFRIGGHELP
AEERSLTGYVAWSKQPKIIAHVADEQGGEGFHRSMHELIKSEIAVPIVCDDEVIAVVCLNSLKPAWFTEEHKRILQIIDR
LTSRHISDVQRIERLEGEVTRLKTDVAYKDPQISSYRLGNIIGNSRKAQEIVDFINTVSVPLFNRITLWSKNVLQEATIG
LPSILVQGQTGAGKEFFFNNLYNKLNEMYREKLNPAGQLPVKKTNIAAYSGDLTYSELFGHKKGAFTGAYSDRKGILEDA
AGGIVFLDEIGDADPKTQVQLLRFLDNGGFVRLGENQDRFSRVLLVAATNKDLAEEIRKGNFREDLYHRLSELAVQVPSL
NERREDIPDLATHFLGKLYRTYRGDESKDAAPTLAEEAKRLLMNHHYHGNIRELRSILLRALFFRKGTVLTADDVRRALA
AGMREFAPATATQELNDRMVTEILDKIANGETFWEAVYEPYSRNAIPRDAVRLVIERSRDAAGRSMPQVARYLKAVGDDV
EENDEERKRFFKFKNFLYKTVKI
>Mature_903_residues
MRLVSGQDRIRIYLEDLTRGVLTCVHASGPLADEIREVSFPIISREASVSSVFVSQYATEFHYEPEGKHTFDRGFAERFA
IGHSYILPVVSQGKSIGVVCVDRFRPGEILRGKGKALLGEFVTSVADRLDVARIYHQQLLLARRVEEYKKREAASFMVQS
AVRLIDRLVLASVLVPVPGPEGSSRLAILASHSEDPSLKKQYDEQGEIALQRGTSLISRFLDDNAVIADERLLRPLFIPD
LTQQELQKKALTEKMALRSLYVVPRYEPSSRKVICLVNYFTKDLYRFSDFEMGLLQTHAEMAERMVNEIGGEHLEIRVLA
EITELLQERNEELSPFLTRVLSMATELIGADTGSIAIVQERDGEKWLVVEDEEGTIVGAKNKSWLKKYIPPFRIGGHELP
AEERSLTGYVAWSKQPKIIAHVADEQGGEGFHRSMHELIKSEIAVPIVCDDEVIAVVCLNSLKPAWFTEEHKRILQIIDR
LTSRHISDVQRIERLEGEVTRLKTDVAYKDPQISSYRLGNIIGNSRKAQEIVDFINTVSVPLFNRITLWSKNVLQEATIG
LPSILVQGQTGAGKEFFFNNLYNKLNEMYREKLNPAGQLPVKKTNIAAYSGDLTYSELFGHKKGAFTGAYSDRKGILEDA
AGGIVFLDEIGDADPKTQVQLLRFLDNGGFVRLGENQDRFSRVLLVAATNKDLAEEIRKGNFREDLYHRLSELAVQVPSL
NERREDIPDLATHFLGKLYRTYRGDESKDAAPTLAEEAKRLLMNHHYHGNIRELRSILLRALFFRKGTVLTADDVRRALA
AGMREFAPATATQELNDRMVTEILDKIANGETFWEAVYEPYSRNAIPRDAVRLVIERSRDAAGRSMPQVARYLKAVGDDV
EENDEERKRFFKFKNFLYKTVKI

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=254, Percent_Identity=38.5826771653543, Blast_Score=165, Evalue=1e-41,
Organism=Escherichia coli, GI87082117, Length=362, Percent_Identity=32.3204419889503, Blast_Score=162, Evalue=1e-40,
Organism=Escherichia coli, GI1788905, Length=237, Percent_Identity=37.1308016877637, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1790299, Length=214, Percent_Identity=42.0560747663551, Blast_Score=154, Evalue=3e-38,
Organism=Escherichia coli, GI1788550, Length=282, Percent_Identity=34.3971631205674, Blast_Score=147, Evalue=4e-36,
Organism=Escherichia coli, GI1789087, Length=359, Percent_Identity=32.033426183844, Blast_Score=146, Evalue=5e-36,
Organism=Escherichia coli, GI1787583, Length=283, Percent_Identity=33.2155477031802, Blast_Score=139, Evalue=1e-33,
Organism=Escherichia coli, GI87082152, Length=226, Percent_Identity=38.0530973451327, Blast_Score=139, Evalue=1e-33,
Organism=Escherichia coli, GI87081872, Length=225, Percent_Identity=37.7777777777778, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1789233, Length=282, Percent_Identity=32.2695035460993, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789828, Length=309, Percent_Identity=31.0679611650485, Blast_Score=124, Evalue=4e-29,
Organism=Escherichia coli, GI1786524, Length=285, Percent_Identity=30.5263157894737, Blast_Score=122, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 102243; Mature: 102243

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVSGQDRIRIYLEDLTRGVLTCVHASGPLADEIREVSFPIISREASVSSVFVSQYATE
CCCCCCCCHHEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHH
FHYEPEGKHTFDRGFAERFAIGHSYILPVVSQGKSIGVVCVDRFRPGEILRGKGKALLGE
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCHHHHHH
FVTSVADRLDVARIYHQQLLLARRVEEYKKREAASFMVQSAVRLIDRLVLASVLVPVPGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGSSRLAILASHSEDPSLKKQYDEQGEIALQRGTSLISRFLDDNAVIADERLLRPLFIPD
CCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCHHCCCC
LTQQELQKKALTEKMALRSLYVVPRYEPSSRKVICLVNYFTKDLYRFSDFEMGLLQTHAE
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHH
MAERMVNEIGGEHLEIRVLAEITELLQERNEELSPFLTRVLSMATELIGADTGSIAIVQE
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
RDGEKWLVVEDEEGTIVGAKNKSWLKKYIPPFRIGGHELPAEERSLTGYVAWSKQPKIIA
CCCCEEEEEECCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEE
HVADEQGGEGFHRSMHELIKSEIAVPIVCDDEVIAVVCLNSLKPAWFTEEHKRILQIIDR
EECCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LTSRHISDVQRIERLEGEVTRLKTDVAYKDPQISSYRLGNIIGNSRKAQEIVDFINTVSV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHCC
PLFNRITLWSKNVLQEATIGLPSILVQGQTGAGKEFFFNNLYNKLNEMYREKLNPAGQLP
HHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
VKKTNIAAYSGDLTYSELFGHKKGAFTGAYSDRKGILEDAAGGIVFLDEIGDADPKTQVQ
CEECCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHH
LLRFLDNGGFVRLGENQDRFSRVLLVAATNKDLAEEIRKGNFREDLYHRLSELAVQVPSL
HHHHHCCCCEEEECCCHHHHHEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
NERREDIPDLATHFLGKLYRTYRGDESKDAAPTLAEEAKRLLMNHHYHGNIRELRSILLR
CHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALFFRKGTVLTADDVRRALAAGMREFAPATATQELNDRMVTEILDKIANGETFWEAVYEP
HHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
YSRNAIPRDAVRLVIERSRDAAGRSMPQVARYLKAVGDDVEENDEERKRFFKFKNFLYKT
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VKI
HCC
>Mature Secondary Structure
MRLVSGQDRIRIYLEDLTRGVLTCVHASGPLADEIREVSFPIISREASVSSVFVSQYATE
CCCCCCCCHHEEHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHH
FHYEPEGKHTFDRGFAERFAIGHSYILPVVSQGKSIGVVCVDRFRPGEILRGKGKALLGE
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCHHHHHH
FVTSVADRLDVARIYHQQLLLARRVEEYKKREAASFMVQSAVRLIDRLVLASVLVPVPGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGSSRLAILASHSEDPSLKKQYDEQGEIALQRGTSLISRFLDDNAVIADERLLRPLFIPD
CCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCHHCCCC
LTQQELQKKALTEKMALRSLYVVPRYEPSSRKVICLVNYFTKDLYRFSDFEMGLLQTHAE
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHH
MAERMVNEIGGEHLEIRVLAEITELLQERNEELSPFLTRVLSMATELIGADTGSIAIVQE
HHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
RDGEKWLVVEDEEGTIVGAKNKSWLKKYIPPFRIGGHELPAEERSLTGYVAWSKQPKIIA
CCCCEEEEEECCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEE
HVADEQGGEGFHRSMHELIKSEIAVPIVCDDEVIAVVCLNSLKPAWFTEEHKRILQIIDR
EECCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
LTSRHISDVQRIERLEGEVTRLKTDVAYKDPQISSYRLGNIIGNSRKAQEIVDFINTVSV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHCC
PLFNRITLWSKNVLQEATIGLPSILVQGQTGAGKEFFFNNLYNKLNEMYREKLNPAGQLP
HHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
VKKTNIAAYSGDLTYSELFGHKKGAFTGAYSDRKGILEDAAGGIVFLDEIGDADPKTQVQ
CEECCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCHHHHH
LLRFLDNGGFVRLGENQDRFSRVLLVAATNKDLAEEIRKGNFREDLYHRLSELAVQVPSL
HHHHHCCCCEEEECCCHHHHHEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
NERREDIPDLATHFLGKLYRTYRGDESKDAAPTLAEEAKRLLMNHHYHGNIRELRSILLR
CHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALFFRKGTVLTADDVRRALAAGMREFAPATATQELNDRMVTEILDKIANGETFWEAVYEP
HHHHHCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
YSRNAIPRDAVRLVIERSRDAAGRSMPQVARYLKAVGDDVEENDEERKRFFKFKNFLYKT
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VKI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]