Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 304570578

GI number: 304570578

Start: 379097

End: 379894

Strand: Direct

Name: fliA [H]

Synonym: NA

Alternate gene names: 304570578

Gene position: 379097-379894 (Clockwise)

Preceding gene: 78355429

Following gene: 78355431

Centisome position: 10.16

GC content: 54.14

Gene sequence:

>798_bases
ATGGCAATATCAAGTTCTTCTGGAAAAAGCTCCTCTTCAGGTAATCAGCCCTGGGAACGGCTGGAGTCCGGTGCTACCGC
GTGGCCCGACTTCACCCCCGCGGAACAGGAACAGGTGGTCAGGCACTATGCGCCCAAGATCAAGTTTCTGGCTTTGCGGC
TCAAGGGCAAACTGCCGAAGAGTGTCGAACTGAACGAGCTGATCAGTGCCGGAACGCTGGGGCTTATGGAAGCCTTCGGC
AAATTCCAGCCCAAGCTCGGCATCAAGTTTGACACCTATGCCGAAAACCGCATTCGCGGGGCCATGCTTGACGAGCTGCG
CAGGCTGGACTGGTTTCCGCGAAGTCTGCGGCAGCGCGTGCGGCTGCTGGAAGACACTATAAACCGTCTGGAGCACGAAA
AAGGGCGGCGTCCTTCGGAAGAAGAACTGCAGAAGGCCACGGGGCTTGAGGAAAAAGACCTGCGTAGTGGCCTGGAAGCA
TTGCAGAATCATCTTTGTATCAGTCTTGATGCCATTCAGGAGACCTTTGCCGCAGACGGCAGAGACCATCTTGAGGGCGA
GCCCTTCCATTCTACAGCCACACAAGAGCTGATCGAGCGCATAGCTTCGCTTATAGATCAATTGACACCAAGGGAAAAGC
TGGTATTGTCGCTATATTATAGTGATGAACTGAACATGCGGGAAACTGCCGAAGTAATGGGTGTTACCGAAGGCAGAGTG
TCGCAGCTTCATTCTCAGGCTCTCAACCGGCTCCGCAAACAGTTTCATGAACAGTTTGGAACTCAGGGCGCCGTTTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: sigma-70 factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:
NA

Sequences:

>Translated_265_residues
MAISSSSGKSSSSGNQPWERLESGATAWPDFTPAEQEQVVRHYAPKIKFLALRLKGKLPKSVELNELISAGTLGLMEAFG
KFQPKLGIKFDTYAENRIRGAMLDELRRLDWFPRSLRQRVRLLEDTINRLEHEKGRRPSEEELQKATGLEEKDLRSGLEA
LQNHLCISLDAIQETFAADGRDHLEGEPFHSTATQELIERIASLIDQLTPREKLVLSLYYSDELNMRETAEVMGVTEGRV
SQLHSQALNRLRKQFHEQFGTQGAV
>Mature_264_residues
AISSSSGKSSSSGNQPWERLESGATAWPDFTPAEQEQVVRHYAPKIKFLALRLKGKLPKSVELNELISAGTLGLMEAFGK
FQPKLGIKFDTYAENRIRGAMLDELRRLDWFPRSLRQRVRLLEDTINRLEHEKGRRPSEEELQKATGLEEKDLRSGLEAL
QNHLCISLDAIQETFAADGRDHLEGEPFHSTATQELIERIASLIDQLTPREKLVLSLYYSDELNMRETAEVMGVTEGRVS
QLHSQALNRLRKQFHEQFGTQGAV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=217, Percent_Identity=42.3963133640553, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789448, Length=207, Percent_Identity=30.9178743961353, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30042; Mature: 29911

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISSSSGKSSSSGNQPWERLESGATAWPDFTPAEQEQVVRHYAPKIKFLALRLKGKLPK
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCC
SVELNELISAGTLGLMEAFGKFQPKLGIKFDTYAENRIRGAMLDELRRLDWFPRSLRQRV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RLLEDTINRLEHEKGRRPSEEELQKATGLEEKDLRSGLEALQNHLCISLDAIQETFAADG
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RDHLEGEPFHSTATQELIERIASLIDQLTPREKLVLSLYYSDELNMRETAEVMGVTEGRV
CHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCHHHHHHHHCCCHHHH
SQLHSQALNRLRKQFHEQFGTQGAV
HHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AISSSSGKSSSSGNQPWERLESGATAWPDFTPAEQEQVVRHYAPKIKFLALRLKGKLPK
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCC
SVELNELISAGTLGLMEAFGKFQPKLGIKFDTYAENRIRGAMLDELRRLDWFPRSLRQRV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RLLEDTINRLEHEKGRRPSEEELQKATGLEEKDLRSGLEALQNHLCISLDAIQETFAADG
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RDHLEGEPFHSTATQELIERIASLIDQLTPREKLVLSLYYSDELNMRETAEVMGVTEGRV
CHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCHHHHHHHHCCCHHHH
SQLHSQALNRLRKQFHEQFGTQGAV
HHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]