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The map label for this gene is sigF [H]

Identifier: 30263977

GI number: 30263977

Start: 3780541

End: 3781440

Strand: Reverse

Name: sigF [H]

Synonym: BA_4115

Alternate gene names: 30263977

Gene position: 3781440-3780541 (Counterclockwise)

Preceding gene: 30263978

Following gene: 30263976

Centisome position: 72.34

GC content: 34.44

Gene sequence:

>900_bases
ATGATGACTGATGATGATTTTATGAAAAAGTATAGCAGGCTAGTTTATTACTATGTAAACAAATATTTTTCCAATATCGC
TAAAGCAATTGAACAAGAAACAAATTTAAATATGGATGATTTAATTCAGTCTGGAATGTTGGGGTTGGTCACAGCGAGAA
GAGATTTTAAAACAGAACTTGGGTTTAAATTTACGACATTCGCTGTTCCAAGAATACTTGGATATATAAGAAAGGCTGTT
AGGGACTCGCAAAAAATTAGGATTACGAACGAAGTGTATGAAATCAAAGGAAAGATTTCTAGAATGAAACTTCATGATGA
AGAAGTGGAAAGTATTGCTAAATTACTAGAAGTTAGCGTGAGAGATGTGAAGACAGCAAAAGAATTTAATCCTAGAACGA
TTTCACTTCAAACACCATTAAATAAAGAGGTAAACAAGGAGAAAGTCACTCTAGAAAATACGCTAATAGATAAAAAAACA
TCAATGACACTCGATGATGTTATTAAGAAAATGATAATCACAGCGTTCTTATCAACCTTATGTAGTAAAGAGAAGTTTGT
ATGGGAACTACATGCTAAAAATATGACACAAGCTGCAATTGGCAAACGGTTAGGTGTAACGCAGACACAAATAAGTAGGA
TTTTAAAAGGGATTTATAAGAAAGCTGAAGAGTATGGAAATAAACATAGCTTACGTGATATAGGCAAGACAAGGTTTGAA
AAAACAATTGAAAATCACGTGAGACAAAGTAGCCATGGGAATTGGTTGGATATAGCTGAAAGTAATGGAGTAAGTAGAGG
AGCGTTTTGGTATAGATTGCGTGAAGGGTGGTCTTACGAGATGGCAGCCACACAACCATTAGGCAGTCAAGGGACAAGGA
AGAATAAGCAAGTTATTTAG

Upstream 100 bases:

>100_bases
TGGGCCTAGGAGCATGAAAAAAGGAGTTATGAAATTAAATTAGGAGGAGAAAACGGTGGGGGATGAACAAAAGGATTTGA
TTGAACAAGAAGATGTTCTA

Downstream 100 bases:

>100_bases
GAGGCAACATGGATAAGCAAAACATTTTGATTAATAAATTAATCGATAATCGTATATACAAGCTTCCAGACGGGCGTGAT
CTATTCGAGGGATCGATCGA

Product: prophage LambdaBa02, RNA polymerase sigma-F factor, putative

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTELGFKFTTFAVPRILGYIRKAV
RDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVNKEKVTLENTLIDKKT
SMTLDDVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEYGNKHSLRDIGKTRFE
KTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI

Sequences:

>Translated_299_residues
MMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTELGFKFTTFAVPRILGYIRKAV
RDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVNKEKVTLENTLIDKKT
SMTLDDVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEYGNKHSLRDIGKTRFE
KTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI
>Mature_299_residues
MMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTELGFKFTTFAVPRILGYIRKAV
RDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVNKEKVTLENTLIDKKT
SMTLDDVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEYGNKHSLRDIGKTRFE
KTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with spoIIAB inhibits sig

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

None

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34569; Mature: 34569

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHC
GFKFTTFAVPRILGYIRKAVRDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RDVKTAKEFNPRTISLQTPLNKEVNKEKVTLENTLIDKKTSMTLDDVIKKMIITAFLSTL
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHH
CSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEYGNKHSLRDIGKTRFE
HCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI
HHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHC
GFKFTTFAVPRILGYIRKAVRDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSV
CCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RDVKTAKEFNPRTISLQTPLNKEVNKEKVTLENTLIDKKTSMTLDDVIKKMIITAFLSTL
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHH
CSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEYGNKHSLRDIGKTRFE
HCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI
HHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3116160; 6088674; 8969508; 9384377; 8358793 [H]