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The map label for this gene is murE

Identifier: 30263916

GI number: 30263916

Start: 3732971

End: 3734446

Strand: Reverse

Name: murE

Synonym: BA_4053

Alternate gene names: 30263916

Gene position: 3734446-3732971 (Counterclockwise)

Preceding gene: 30263917

Following gene: 30263915

Centisome position: 71.44

GC content: 37.47

Gene sequence:

>1476_bases
ATGAAGTTGCATACACTTGTATCATGTTTGCATGATTTTCCAGTTGTTCCAAAAGAAAATCCAGAAATCACATCTATAGA
AGCTGATTCACGTAAAGTGAAAGAGGGAAGCTTATTTGTATGTATGAAAGGGTATACGGTTGATAGCCATGATTTTGCTA
AGCAAGCAGCGGCACAAGGAGCGGCTGCTATTGTTGCGGAAAGACCAATTGATGTTGACGTTCCAGTTGTACTTGTGAAA
AACACTTTTCGTTCGTTAGCGGTTTTAGCTGATTATTTTTATGGTCAACCAACACACAAGTTACATTTAATCGGTATTAC
AGGTACAAATGGAAAGACAACAACATCGCATATTATGGATGAGATTATGCGCGCACATGGTCATAAAACGGGGCTAATTG
GAACGATTAATATGAAAATCGGTGATGAAACATTTGAGGTGAAAAATACAACGCCAGATGCACTAACACTTCAACAGACG
TTTGCAAAAATGGTTGAACACGGTGTGGATAGCACAGTAATGGAAGTTTCATCACATGCTTTAGATCTTGGTCGTGTACA
CGGATGTGATTACGATGTAGCGGTGTTTACAAATTTAACACAAGATCATTTAGACTACCATAAAACGATGGAAGAATATA
AACATGCAAAAGGGTTGCTATTTGCACAACTTGGCAATAGTTATAATCATAATCGCGAAAAATACGCGGTACTGAATAAC
GATGATGCTGTAACAGTGGAATATATGAGAAGTACTGCAGCAACTGTTGTAACGTATGGAATTGATACAACAAGTGATGT
TATGGCCAAAGATATCGCTATGACAAGTGGTGGAACTACATTTACGCTTGTAACACCGTATGAAAGTGTAAATGTTACGA
TGAAATTAATTGGGAAATTTAATGTATATAATGTACTAGCTGCAACAGCGGCAGGACTTGTTTCTGGTGTAAGTTTGGAA
ACAATCATTGATGTAATAAAGGAACTAGCTGGAGTTCCTGGACGTTTCGAAGTAGTTGATGGTGGCCAAAATTATACAGT
TATTGTTGATTATGCACATACACCAGATAGCTTAGAAAATGTATTGAAAACAGCAAAGCAGTTTGCAAAAGGTGACGTAT
ATTGCATCGTCGGTTGTGGTGGTGATAGAGATCGAACGAAGAGACCAATCATGGCAAGTGTTGCCACACAATATGCAACT
CACGCAATTTACACTTCAGATAATCCAAGAAGTGAAGATCCAGCGGCTATTTTAGATGATATGGTACATGGTGCAAATGG
AAATAATTATGAAGTGATTATTGATAGAAAAGAAGCAATACACCATGCGATCACTAAAGCGAAAGCAGATGATATCATTA
TTATTGCTGGTAAGGGTCATGAAACATATCAAATTATTGGTAAAGATGTTCATCATTTTGACGATCGTGAAGTCGCTAAA
GAAGCAATTACAGAGCGTTTAAACAACGAAGAGTAA

Upstream 100 bases:

>100_bases
AGACACAGTAGCGCTTACCTCCTATTTTACATAATTAGGAGGTAGCGTGTTGCTTATTAATAAAAGAGATAAATACACAA
TGTAGAGAGGGTTTAGTGAA

Downstream 100 bases:

>100_bases
CAACGTGAGAGGAGGACTACATGTGCTTGAGCAAGGTTTATTAGTAACGGCTGGGGTAGCATTCTTAATTTCTGTTGCCC
TTTCGCCATTGTTTATTCCA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK
NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT
FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
DDAVTVEYMRSTAATVVTYGIDTTSDVMAKDIAMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE
TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYAT
HAIYTSDNPRSEDPAAILDDMVHGANGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
EAITERLNNEE

Sequences:

>Translated_491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK
NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT
FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
DDAVTVEYMRSTAATVVTYGIDTTSDVMAKDIAMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE
TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYAT
HAIYTSDNPRSEDPAAILDDMVHGANGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
EAITERLNNEE
>Mature_491_residues
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQGAAAIVAERPIDVDVPVVLVK
NTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMDEIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQT
FAKMVEHGVDSTVMEVSSHALDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
DDAVTVEYMRSTAATVVTYGIDTTSDVMAKDIAMTSGGTTFTLVTPYESVNVTMKLIGKFNVYNVLAATAAGLVSGVSLE
TIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLENVLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYAT
HAIYTSDNPRSEDPAAILDDMVHGANGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
EAITERLNNEE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=480, Percent_Identity=35.625, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1786274, Length=339, Percent_Identity=28.9085545722714, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_BACAN (Q81WC7)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_846293.1
- RefSeq:   YP_020695.1
- RefSeq:   YP_030016.1
- ProteinModelPortal:   Q81WC7
- SMR:   Q81WC7
- EnsemblBacteria:   EBBACT00000008767
- EnsemblBacteria:   EBBACT00000016254
- EnsemblBacteria:   EBBACT00000023692
- GeneID:   1086243
- GeneID:   2815156
- GeneID:   2848185
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4053
- KEGG:   bar:GBAA_4053
- KEGG:   bat:BAS3765
- TIGR:   BA_4053
- TIGR:   GBAA_4053
- GeneTree:   EBGT00050000000629
- HOGENOM:   HBG602753
- OMA:   IGTIANY
- ProtClustDB:   PRK00139
- BioCyc:   BANT260799:BAS3765-MONOMER
- BioCyc:   BANT261594:GBAA4053-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 53705; Mature: 53705

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 149-149 BINDING 177-177 BINDING 185-185 BINDING 383-383 BINDING 457-457 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQG
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEECCHHHHHHHHHCC
AAAIVAERPIDVDVPVVLVKNTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMD
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH
EIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQTFAKMVEHGVDSTVMEVSSHA
HHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
LDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECC
DDAVTVEYMRSTAATVVTYGIDTTSDVMAKDIAMTSGGTTFTLVTPYESVNVTMKLIGKF
CCEEEHHHHHHCCEEEEEECCCCCHHHHHHHHEEECCCCEEEEECCCCCCCEEEEEEECC
NVYNVLAATAAGLVSGVSLETIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLEN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
VLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYATHAIYTSDNPRSEDPAAILDD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
MVHGANGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
HHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCHHHHHH
EAITERLNNEE
HHHHHHHCCCC
>Mature Secondary Structure
MKLHTLVSCLHDFPVVPKENPEITSIEADSRKVKEGSLFVCMKGYTVDSHDFAKQAAAQG
CCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCEECCHHHHHHHHHCC
AAAIVAERPIDVDVPVVLVKNTFRSLAVLADYFYGQPTHKLHLIGITGTNGKTTTSHIMD
CEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHH
EIMRAHGHKTGLIGTINMKIGDETFEVKNTTPDALTLQQTFAKMVEHGVDSTVMEVSSHA
HHHHHCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
LDLGRVHGCDYDVAVFTNLTQDHLDYHKTMEEYKHAKGLLFAQLGNSYNHNREKYAVLNN
HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECC
DDAVTVEYMRSTAATVVTYGIDTTSDVMAKDIAMTSGGTTFTLVTPYESVNVTMKLIGKF
CCEEEHHHHHHCCEEEEEECCCCCHHHHHHHHEEECCCCEEEEECCCCCCCEEEEEEECC
NVYNVLAATAAGLVSGVSLETIIDVIKELAGVPGRFEVVDGGQNYTVIVDYAHTPDSLEN
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEECCCCHHHHH
VLKTAKQFAKGDVYCIVGCGGDRDRTKRPIMASVATQYATHAIYTSDNPRSEDPAAILDD
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
MVHGANGNNYEVIIDRKEAIHHAITKAKADDIIIIAGKGHETYQIIGKDVHHFDDREVAK
HHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHCCCHHHHHH
EAITERLNNEE
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629