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The map label for this gene is nemA [H]

Identifier: 30263593

GI number: 30263593

Start: 3408093

End: 3409220

Strand: Reverse

Name: nemA [H]

Synonym: BA_3707

Alternate gene names: 30263593

Gene position: 3409220-3408093 (Counterclockwise)

Preceding gene: 30263594

Following gene: 30263592

Centisome position: 65.22

GC content: 39.72

Gene sequence:

>1128_bases
ATGACAAGTTCAAACAAGAAATCGGCAAGTATTTATGAAGGAACAAATGTAAACGCTTGGGGGTCTTTTGAAAACGTTGC
AGAAACGAAGTTGTTTAATTCGATTCAAATTGGATCTTGGCCTCTTCGTAACCGTATAGCAATGGCACCAATGACAAGAT
GCTTTGCAGACAATGAAACTGGAGCAGTGGGTGCTGATGTAGTGGAATACTATAGAAAACGTGCAGCTGATGGAGTTGGA
TTGATTATTACAGAGGGAATCGTCATTAGCCCAAGAGCAAAAGGGAATCCGGGAGTACCGGGGATTTATACACAAGAACA
AATTGATTCGTGGAAACCTGTTACAGAGGCAGTACATAAAGAAGGCGGGACAATTATTGCTCAAATATGGCATGTTGGTC
GTATGAGTCATCATGAAATAATTGGTGGGCAAATGCCGCAAGCGCCGTCTGCAATCGCTGCAGAGGGAAATGTTCCACGT
TTTCGTAAGCCATTCGATACACCAGAAGCAATGACGTTAGAAGAAATACAAGAAGTTATCGGTCAATACGCACAAGCTGC
AAAAAATGCGATCGAAGCTGGGTTTGATGGAGTAGAAATTCACGGTGCACACGGATATTTAATTGATCAATTTACTTATG
AGTTTGTAAACAAGCGTACAGATAAATATGGTGGCGACTTAAAGCAAAGGTTAACGTTTATGAAAGAAGTAACTGAGGCT
GTAATAGAAGCGATTGGAGCTGATAAAACACTTCTTCGTTTCTCTGCTTTTAAAGGTGATAATCCTACTTATATGTGGGA
AAACCCTGAACTGGCAATTGAAACATTCGTAAATATGTTTAAAGAAGTTGGATTAACGATGATTCACCCTTCAACGATGA
GTTATACGCAAGTGATTGCTGACGGAAAGAATTTTCATCAATTAGTAAGAAAATATTGGGATGGTGCGATTGTTGGGGTT
GGTAATTTGAATCCGAAAGAGGCTGAAGAAGCGTTGCAAGAAGGAACAATTGATGTAGCTGCATTCGGAAGACCTTTAAT
TGCTAATCCGGATTTTGTTCATCGAATTAAAAGTGCTGAAAGTTTAGTTGAATATGACGCGAAAGAGCATCTTGCTACAT
TGATTTGA

Upstream 100 bases:

>100_bases
AATATTCGAATAACCTCTGGTTAAAGCGTATAAATTGACGTTACTATCTAAAAGTAAGTATGGTGCGATTGTAGTAAGAA
TTTTTTTAGGGGGAAGAAAC

Downstream 100 bases:

>100_bases
ATAAAGATAAAAGGAGAACACACGGAGTGTTCTCCTTTTATTATGGGATTTCATAGGGGGAAGCGAGATGAGTGAAACGC
TACTCGTTATTATTAGTATA

Product: Oye family NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVG
LIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFVNKRTDKYGGDLKQRLTFMKEVTEA
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Sequences:

>Translated_375_residues
MTSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVG
LIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPR
FRKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFVNKRTDKYGGDLKQRLTFMKEVTEA
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGV
GNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI
>Mature_374_residues
TSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNETGAVGADVVEYYRKRAADGVGL
IITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHKEGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRF
RKPFDTPEAMTLEEIQEVIGQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFVNKRTDKYGGDLKQRLTFMKEVTEAV
IEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIADGKNFHQLVRKYWDGAIVGVG
NLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAESLVEYDAKEHLATLI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=345, Percent_Identity=38.2608695652174, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789463, Length=345, Percent_Identity=31.304347826087, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17565138, Length=349, Percent_Identity=28.9398280802292, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=359, Percent_Identity=23.9554317548747, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=310, Percent_Identity=25.1612903225806, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=191, Percent_Identity=30.3664921465969, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=326, Percent_Identity=25.7668711656442, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=339, Percent_Identity=25.3687315634218, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=29.3023255813954, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321973, Length=363, Percent_Identity=31.6804407713499, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325086, Length=365, Percent_Identity=31.7808219178082, Blast_Score=162, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41368; Mature: 41237

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNET
CCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHCCCCC
GAVGADVVEYYRKRAADGVGLIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFVNKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
DGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAE
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSNKKSASIYEGTNVNAWGSFENVAETKLFNSIQIGSWPLRNRIAMAPMTRCFADNET
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHCCCCC
GAVGADVVEYYRKRAADGVGLIITEGIVISPRAKGNPGVPGIYTQEQIDSWKPVTEAVHK
CCCHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHH
EGGTIIAQIWHVGRMSHHEIIGGQMPQAPSAIAAEGNVPRFRKPFDTPEAMTLEEIQEVI
CCCEEEHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GQYAQAAKNAIEAGFDGVEIHGAHGYLIDQFTYEFVNKRTDKYGGDLKQRLTFMKEVTEA
HHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VIEAIGADKTLLRFSAFKGDNPTYMWENPELAIETFVNMFKEVGLTMIHPSTMSYTQVIA
HHHHHCCCHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHH
DGKNFHQLVRKYWDGAIVGVGNLNPKEAEEALQEGTIDVAAFGRPLIANPDFVHRIKSAE
CCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
SLVEYDAKEHLATLI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]