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The map label for this gene is opuE [H]

Identifier: 30263591

GI number: 30263591

Start: 3404691

End: 3406169

Strand: Reverse

Name: opuE [H]

Synonym: BA_3705

Alternate gene names: 30263591

Gene position: 3406169-3404691 (Counterclockwise)

Preceding gene: 30263592

Following gene: 30263590

Centisome position: 65.16

GC content: 36.58

Gene sequence:

>1479_bases
GTGAAGATTGAGATTATGGTTTCGCTTGCTATTTATATGGCAGGTATGTTGTATATCGGGTATTGGTCTTATAAGAAGAC
ATCCGATTTATCAGATTATATGTTAGGCGGAAGAGGACTCGGTCCAGCAGTTACAGCTTTATCAGCCGGTGCTTCTGACA
TGAGCGGTTGGATGCTTATGGGATTACCGGGTGCGATGTATGCGACAGGGTTGTCCAGTGTATGGATCGCGATAGGTTTA
TTAATAGGCGCTTATGCAAACTATTTAATTCTTGCGCCGCGTTTACGAACATATACGGAAGTAGCAAATGATTCAATTAC
GATTCCAGATTTTTTAGAGAATCGGTTTAAAGATCGTACGAAAATACTTCGTTTTGTCTCCGCTATCGTCATTTTAGTAT
TTTTCACATTTTATGCGTCAGCTGGTTTAGTTTCAGGTGGACGTTTGTTTGAAAATTCTTTTAACCTTGATTATAAAATA
GGTTTATTTGTAACTGTTGGTGTCGTTGTTGCTTATACACTATTCGGTGGTTTTTTAGCAGTAAGTTGGACCGACTTTGT
GCAAGGTTGTATTATGTTTATTGCTCTTGTATTAGTTCCAATTGTAGCTTTTACAGATGTCGGTGGTGTAACAGAAACAT
TCAATACAATTAAGCAAGTTGATGCATCGCATTTAGATATGTTTAAAGGGACTACAATACTTGGTATTATTTCATTTTTA
GCATGGGGCCTTGGGTATTTTGGTCAACCACATATTATTGTCCGCTTTATGGCAATTACCTCTATTAAAGATTTAAAAAC
TTCTCGTAGAATTGGTATCGGTTGGATGACGATTTCAATTATAGGTGCAATGCTTACTGGTCTAGTTGGTATTGCTTATT
ACGCTAAAAATAATGCGACATTACAAGATCCGGAAATGGTCTTTGTAACATTCTCAAATATTTTATTCCATCCGTACATT
ACTGGATTTTTATTATCAGCTATTTTGGCTTCGATTATGAGTAGTATTTCCTCGCAATTACTTGTTATTTCAAGTGCTGT
AACGGAAGATTTCTATAAAACATTTTTCCGTCGTAAAGCAAGTGATAAAGAACTTGTATTTATCGGTAGGCTGTCAGTAT
TAGTAGTAGCGATGATTGCAGTTGTTTTAGCGTATCATCCGAGTGATACAATTTTAACGCTTGTTGGATATGCTTGGGCA
GGATTTGGATCAGCATTCGGACCAGCAATTTTATTAAGTTTATATTGGAAGAGAACGAACAAATGGGGCGTTCTTGCTGG
GATGATTGTCGGTGCATTAGTTGTTATCACTTGGGTACAAATTCCAAGTTTAAAAGCGAGTATGTATGAGATGGTACCTG
GATTCTTCTGTAGCTTATTAGCTGTTATTATCGTAAGTTTAGTAACGAAAGAACCAGTTAAAGCAATACATCGTGAATTT
AATGAGATGGAAGCAGTATTGGAAGAGGAAACAAAATAA

Upstream 100 bases:

>100_bases
AAATGTTTGGTAAGGTAGGGAACCATAAGCGAAAACAAGAAAATAGAAAAAGTCATTTTTCGAAATTTTGAATGGCTTAT
GTAACTAAGGGAGAGATTTG

Downstream 100 bases:

>100_bases
TTAGTGAAGAGGGTAAACGCTGAATAGCGTTTACCTTCTTTTCATTTTAAATGAAATCCGTAACTTATTGACAAAGAAAA
GTAAGTAACTTATAATAAAT

Product: sodium/proline symporter family protein

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIGL
LIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKI
GLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFL
AWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPYI
TGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWA
GFGSAFGPAILLSLYWKRTNKWGVLAGMIVGALVVITWVQIPSLKASMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHREF
NEMEAVLEEETK

Sequences:

>Translated_492_residues
MKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIGL
LIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKI
GLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFL
AWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPYI
TGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWA
GFGSAFGPAILLSLYWKRTNKWGVLAGMIVGALVVITWVQIPSLKASMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHREF
NEMEAVLEEETK
>Mature_492_residues
MKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLMGLPGAMYATGLSSVWIAIGL
LIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRTKILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKI
GLFVTVGVVVAYTLFGGFLAVSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFL
AWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNATLQDPEMVFVTFSNILFHPYI
TGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKASDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWA
GFGSAFGPAILLSLYWKRTNKWGVLAGMIVGALVVITWVQIPSLKASMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHREF
NEMEAVLEEETK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=494, Percent_Identity=55.0607287449393, Blast_Score=540, Evalue=1e-154,
Organism=Homo sapiens, GI11141885, Length=488, Percent_Identity=23.9754098360656, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI14140236, Length=539, Percent_Identity=23.747680890538, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI110835708, Length=545, Percent_Identity=22.9357798165138, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI109659836, Length=381, Percent_Identity=22.5721784776903, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI4507031, Length=470, Percent_Identity=24.2553191489362, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI109659839, Length=397, Percent_Identity=21.9143576826196, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI206597483, Length=464, Percent_Identity=23.0603448275862, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI206597487, Length=464, Percent_Identity=23.2758620689655, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787251, Length=485, Percent_Identity=49.4845360824742, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI87082237, Length=460, Percent_Identity=29.5652173913043, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1790503, Length=454, Percent_Identity=27.0925110132159, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1790113, Length=391, Percent_Identity=24.0409207161125, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539284, Length=447, Percent_Identity=25.2796420581655, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533094, Length=384, Percent_Identity=21.875, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648033, Length=452, Percent_Identity=24.1150442477876, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356865, Length=452, Percent_Identity=24.1150442477876, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221459584, Length=372, Percent_Identity=24.1935483870968, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24640370, Length=358, Percent_Identity=23.7430167597765, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24645928, Length=375, Percent_Identity=25.6, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281362918, Length=297, Percent_Identity=26.9360269360269, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24651741, Length=466, Percent_Identity=24.8927038626609, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24651739, Length=449, Percent_Identity=24.0534521158129, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221459588, Length=380, Percent_Identity=23.1578947368421, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221459586, Length=385, Percent_Identity=22.0779220779221, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53893; Mature: 53893

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLM
CEEEEHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEEE
GLPGAMYATGLSSVWIAIGLLIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCHHHHHHHHHHHH
KILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAYTLFGGFLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
VSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHH
AWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNAT
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LQDPEMVFVTFSNILFHPYITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKA
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILLSLYWKRTN
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
KWGVLAGMIVGALVVITWVQIPSLKASMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHREF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEMEAVLEEETK
HHHHHHHHHCCC
>Mature Secondary Structure
MKIEIMVSLAIYMAGMLYIGYWSYKKTSDLSDYMLGGRGLGPAVTALSAGASDMSGWMLM
CEEEEHHHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCCHHHHHHHCCCCCCCCEEEE
GLPGAMYATGLSSVWIAIGLLIGAYANYLILAPRLRTYTEVANDSITIPDFLENRFKDRT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHCCCCCCCHHHHHHHHHHHH
KILRFVSAIVILVFFTFYASAGLVSGGRLFENSFNLDYKIGLFVTVGVVVAYTLFGGFLA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
VSWTDFVQGCIMFIALVLVPIVAFTDVGGVTETFNTIKQVDASHLDMFKGTTILGIISFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHH
AWGLGYFGQPHIIVRFMAITSIKDLKTSRRIGIGWMTISIIGAMLTGLVGIAYYAKNNAT
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LQDPEMVFVTFSNILFHPYITGFLLSAILASIMSSISSQLLVISSAVTEDFYKTFFRRKA
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SDKELVFIGRLSVLVVAMIAVVLAYHPSDTILTLVGYAWAGFGSAFGPAILLSLYWKRTN
CCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
KWGVLAGMIVGALVVITWVQIPSLKASMYEMVPGFFCSLLAVIIVSLVTKEPVKAIHREF
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NEMEAVLEEETK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]