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The map label for this gene is rihA [H]

Identifier: 30263499

GI number: 30263499

Start: 3315233

End: 3316165

Strand: Reverse

Name: rihA [H]

Synonym: BA_3606

Alternate gene names: 30263499

Gene position: 3316165-3315233 (Counterclockwise)

Preceding gene: 30263500

Following gene: 30263498

Centisome position: 63.44

GC content: 36.23

Gene sequence:

>933_bases
ATGAAAAAAGTATTATTTTTAGGAGACCCAGGAATTGATGACTCTTTAGCAATTATGTATGGATTGTTGCATCCTGATAT
TGATATTGTTGGTGTAGTAACTGGATATGGAAATGTAACGCAAGAAAAGGCGACAAGTAATGCGGCATATTTATTGCAAC
TGGCAGGACGGGAAGATATACCTATTATTAATGGTGCGAAAATCCCTTTATCTGGAGATATTACAACGTATTATCCAGAA
ATTCATGGGGCGGAAGGCTTAGGACCAATTCGACCGCCGAAAAATCTTTCTCCAAATATAAGGCCTTTTTGTGAGTTTTT
TGACATTCTTGAAAAATATAAAGGAGAATTAATTATAGTTGATGCTGGGAGGTCAACGACACTTGCAACAGCATTTATTT
TAGAAAAACCATTGATGAAGTATGTGAAAGAATATTATATAATGGGCGGTGCTTTTTTAATGCCTGGAAATGTTACACCA
GTCGCAGAAGCGAATTTTCATGGTGACCCTATTGCATCACAATTAGTCATGCAAAATGCCAAGAATGTGACGTTGGTGCC
GCTGAATGTTACATCTGAAGCTATAATCACGCCAGAGATGGTAAAGTACATTACGAAACATTCTAAAACGAGTTTTAATA
AATTAATTGAACCGATTTTTACGTATTATTATAAAGCTTATAGAAAGTTAAATCCGAAAATAACAGGAAGTCCAGTACAT
GACGTTGTTACAATGATGGTCGCGGCGAATCCTTCAATACTGGATTATGTGTATCGTCGTGTAGATGTAGATACAGTGGG
GATTGCAAAAGGAGAAAGTATTGCAGATTTCCGTCCTCAACCTGATGCAAAAGCCTTAAAAAATTGGGTACGAATTGGTT
GGTCATTACATTATAAAAAATTCCTTGAGGATTTTGTGAAAATCATGACGTAG

Upstream 100 bases:

>100_bases
TTACAGGACTGCGTTTTTTCGTATAAATTTGTTCTTTTTATTCTGTAATCCTGTGAATATTCATAAATTAATAACAATTG
AGGGGATGGAGTTGAGGGCG

Downstream 100 bases:

>100_bases
ACATAATGGGAGTATACAAGTTAAAATAATGGAGATGGTTATTCAAATAACCATCTCCATTATTTTGTTTTTTTAAGATA
GAGAGAAAGAGTTACACAAT

Product: inosine-uridine preferring nucleoside hydrolase family protein

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTYYPE
IHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTP
VAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Sequences:

>Translated_310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTYYPE
IHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTP
VAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT
>Mature_310_residues
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDIPIINGAKIPLSGDITTYYPE
IHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIVDAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTP
VAEANFHGDPIASQLVMQNAKNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKKFLEDFVKIMT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=280, Percent_Identity=29.2857142857143, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788486, Length=279, Percent_Identity=27.2401433691756, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=28.4313725490196, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI32565474, Length=198, Percent_Identity=28.7878787878788, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17565698, Length=200, Percent_Identity=29, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=275, Percent_Identity=30.5454545454545, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34465; Mature: 34465

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDI
CCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCCCCC
PIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIV
CEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
DAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVMQNA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
KNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVH
CCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKK
HHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLEDFVKIMT
HHHHHHHHCC
>Mature Secondary Structure
MKKVLFLGDPGIDDSLAIMYGLLHPDIDIVGVVTGYGNVTQEKATSNAAYLLQLAGREDI
CCEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCCEEEEEECCCCCC
PIINGAKIPLSGDITTYYPEIHGAEGLGPIRPPKNLSPNIRPFCEFFDILEKYKGELIIV
CEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEE
DAGRSTTLATAFILEKPLMKYVKEYYIMGGAFLMPGNVTPVAEANFHGDPIASQLVMQNA
ECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
KNVTLVPLNVTSEAIITPEMVKYITKHSKTSFNKLIEPIFTYYYKAYRKLNPKITGSPVH
CCEEEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
DVVTMMVAANPSILDYVYRRVDVDTVGIAKGESIADFRPQPDAKALKNWVRIGWSLHYKK
HHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FLEDFVKIMT
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA