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The map label for this gene is yofA [H]

Identifier: 30261969

GI number: 30261969

Start: 1822857

End: 1823645

Strand: Reverse

Name: yofA [H]

Synonym: BA_1937

Alternate gene names: 30261969

Gene position: 1823645-1822857 (Counterclockwise)

Preceding gene: 30261981

Following gene: 30261958

Centisome position: 34.89

GC content: 31.05

Gene sequence:

>789_bases
ATGGAAATAAATGATCTTATTATATTCAAAACTGTAGCTAGCGAAGGTTCTATTAGTAAAGCTGCGAAAGAGTTAGGATA
TGTTCAACCAAACGTAACTGAGCGAATCAAAAAATTAGAACTAGAATTAGAAACACCTTTACTACATAGAGATAACAAAG
GCGTTTCGTTGTTACCTTCTGGTGATATTTTACTAGACTACACAAATCAAATATTAACGCTAGTAGAAGAAGCGAGAGAT
AAAATTAAAATGTGTGGTTCTTCTTATGTAATAGCGACGTCACAATCAATTTTGACTAATTATGTAAGTAAGCGCATTAA
AGAAAATTTCAGGAATTATCAAATATATGTAGAAAATAGTAGTCATTTGCAAAAATTACTGCAGCAACAAAAAGTTGATA
TGGTCATTACTTATGAGGATTACCCGGACACAGCGTTTAAAAAAGTATTTTCCACTTCAATTTCTATCGGTTTATTAAAA
GCGAAAGAAAAGTGTACCGTTGACTATTCAAAGGAACTCTTCTTCGTTAGCAATGACAAAAAGTGCCCTTTTAGAAATAA
GACCATTCAATTTCTAAAAGAAAATAATTTATCTCAGCGTCAACTTCAACAACTACATTCTTATTCGCTTATGGAAGAGT
ATATTCAGGACGGAAAAGGCATAGCCTTTTTACCGATTAATAATGATAAATTGGTACCAATTGAAGATGTTCAAATTGAG
AAATTAACTGTCCATTTTTTCACAAATCAAAACTCTTCAAAACACATCCCTGATGAATTATTTAATTAA

Upstream 100 bases:

>100_bases
CCTATATACATAATGATAAAGCGATGACTGACTAACGTAAAATAGGTATAACTTATGCTTGGTATCATTTATAATGATAT
CGTATAAGGAGCTGTGATAA

Downstream 100 bases:

>100_bases
TAAAAAATCCCTCCTTTACGAATCTAAAAGAGGGATTTTCATATTTTATACAATCGATTTTTCACACGTTTTGCTCCACT
TCTGCACGTTTCTCCTTCTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPSGDILLDYTNQILTLVEEARD
KIKMCGSSYVIATSQSILTNYVSKRIKENFRNYQIYVENSSHLQKLLQQQKVDMVITYEDYPDTAFKKVFSTSISIGLLK
AKEKCTVDYSKELFFVSNDKKCPFRNKTIQFLKENNLSQRQLQQLHSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
KLTVHFFTNQNSSKHIPDELFN

Sequences:

>Translated_262_residues
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPSGDILLDYTNQILTLVEEARD
KIKMCGSSYVIATSQSILTNYVSKRIKENFRNYQIYVENSSHLQKLLQQQKVDMVITYEDYPDTAFKKVFSTSISIGLLK
AKEKCTVDYSKELFFVSNDKKCPFRNKTIQFLKENNLSQRQLQQLHSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
KLTVHFFTNQNSSKHIPDELFN
>Mature_262_residues
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPSGDILLDYTNQILTLVEEARD
KIKMCGSSYVIATSQSILTNYVSKRIKENFRNYQIYVENSSHLQKLLQQQKVDMVITYEDYPDTAFKKVFSTSISIGLLK
AKEKCTVDYSKELFFVSNDKKCPFRNKTIQFLKENNLSQRQLQQLHSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
KLTVHFFTNQNSSKHIPDELFN

Specific function: Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=240, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30199; Mature: 30199

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPS
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEECCCCCEEEECC
GDILLDYTNQILTLVEEARDKIKMCGSSYVIATSQSILTNYVSKRIKENFRNYQIYVENS
CCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECC
SHLQKLLQQQKVDMVITYEDYPDTAFKKVFSTSISIGLLKAKEKCTVDYSKELFFVSNDK
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCEEECCCEEEEEECCC
KCPFRNKTIQFLKENNLSQRQLQQLHSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCEEE
KLTVHFFTNQNSSKHIPDELFN
EEEEEEEECCCCCCCCCHHHCC
>Mature Secondary Structure
MEINDLIIFKTVASEGSISKAAKELGYVQPNVTERIKKLELELETPLLHRDNKGVSLLPS
CCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHEECCCEEECCCCCEEEECC
GDILLDYTNQILTLVEEARDKIKMCGSSYVIATSQSILTNYVSKRIKENFRNYQIYVENS
CCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEEECC
SHLQKLLQQQKVDMVITYEDYPDTAFKKVFSTSISIGLLKAKEKCTVDYSKELFFVSNDK
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHEEEEECCCCCEEECCCEEEEEECCC
KCPFRNKTIQFLKENNLSQRQLQQLHSYSLMEEYIQDGKGIAFLPINNDKLVPIEDVQIE
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCEEE
KLTVHFFTNQNSSKHIPDELFN
EEEEEEEECCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]