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The map label for this gene is fusA

Identifier: 30065374

GI number: 30065374

Start: 4330060

End: 4332027

Strand: Direct

Name: fusA

Synonym: S4404

Alternate gene names: 30065374

Gene position: 4330060-4332027 (Clockwise)

Preceding gene: 30065373

Following gene: 30065375

Centisome position: 94.14

GC content: 50.86

Gene sequence:

>1968_bases
ATGGACTGGATGGAGCAGGAGCAGGAACGTGGTATTACCATCACTTCCGCTGCGACTACTGCATTCTGGTCTGGTATGGC
TAAGCAGTATGAGCCGCATCGCATCAACATCATCGACACCCCGGGGCACGTTGACTTCACAATCGAAGTAGAACGTTCCA
TGCGTGTTCTCGATGGTGCGGTAATGGTTTACTGCGCAGTTGGTGGTGTTCAGCCGCAGTCTGAAACCGTATGGCGTCAG
GCAAACAAATATAAAGTTCCGCGCATTGCGTTCGTTAACAAAATGGACCGCGTGGGTGCGAACTTCCTGAAAGTTGTTAA
CCAGATCAAAACCCGTCTGGGCGCGAACCCGGTTCCGCTGCAGCTGGCGATTGGTGCTGAAGAACATTTCACCGGTGTTG
TTGACCTGGTGAAAATGAAAGCTATCAACTGGAATGACGCTGACCAGGGCGTAACCTTCGAATACGAAGATATCCCGGCA
GACATGGTTGAACTGGCTAACGAATGGCACCAGAACCTGATCGAATCCGCAGCTGAAGCTTCTGAAGAGCTGATGGAAAA
ATACCTGGGTGGTGAAGAACTGACTGAAGCAGAAATCAAAGGTGCTCTGCGTCAGCGCGTTCTGAACAACGAAATCATCC
TGGTAACCTGTGGTTCTGCGTTCAAGAACAAAGGTGTTCAGGCGATGCTGGATGCGGTAATTGATTACCTGCCATCCCCG
GTTGACGTACCTGCGATCAACGGTATCCTGGACGACGGTAAAGACACTCCGGCTGAACGTCACGCAAGTGATGACGAGCC
GTTCTCTGCACTGGCGTTCAAAATCGCTACCGACCCGTTTGTTGGTAACCTGACCTTCTTCCGTGTTTACTCCGGTGTGG
TTAACTCTGGTGATACCGTACTGAACTCCGTGAAAGCTGCACGTGAGCGTTTCGGTCGTATCGTTCAGATGCACGCTAAC
AAACGTGAAGAGATCAAAGAAGTTCGCGCGGGCGACATCGCTGCTGCTATCGGCCTGAAAGACGTAACCACTGGTGACAC
CCTGTGTGACCCGGATGCGCCGATCATTCTGGAACGTATGGAATTCCCTGAGCCGGTAATCTCCATCGCAGTTGAACCGA
AAACCAAAGCTGACCAGGAAAAAATGGGTCTGGCTCTGGGCCGTCTGGCTAAAGAAGACCCGTCTTTCCGTGTATGGACT
GACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGA
ATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGCCAGAAAGTTACCGATGTTGAAG
GTAAACACGCGAAACAGTCTGGTGGTCGTGGTCAGTATGGTCATGTTGTTATCGACATGTACCCGCTGGAGCCGGGTTCA
AACCCGAAAGGCTACGAGTTCATCAACGACATTAAAGGTGGTGTAATCCCTGGCGAATACATCCCGGCCGTTGATAAAGG
TATCCAGGAACAGCTGAAAGCAGGTCCGCTGGCAGGCTACCCGGTAGTAGACATGGGTATTCGTCTGCACTTCGGTTCTT
ACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAA
CCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGAACACCGGTGACGTTATCGGTGACTTGAG
CCGTCGTCGTGGTATGCTCAAAGGTCAGGAATCTGAAGTTACTGGCGTTAAGATCCACGCTGAAGTACCGCTGTCTGAAA
TGTTCGGATACGCAACTCAGCTGCGTTCTCTGACCAAAGGTCGTGCATCATACACTATGGAATTCCTGAAGTATGATGAA
GCGCCGAGTAACGTTGCTCAGGCCGTAATTGAAGCCCGTGGTAAATAA

Upstream 100 bases:

>100_bases
TGCGCACATCGACGCCGGTAAAACCACTACTACCGAACGTATTCTGTTCTACACCGGTGTAAACCATAAAATCGGTGAAG
TTCATGACGGCGCTGCAACC

Downstream 100 bases:

>100_bases
GCCTAAGGGTTAATACCAAAGTCCCGTGCTCTCTCCTGAAGGGGAGAGCACTATAGTAAGGAATATAGCCGTGTCTAAAG
AAAAATTTGAACGTACAAAA

Product: elongation factor EF-2

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQ
ANKYKVPRIAFVNKMDRVGANFLKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPA
DMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSP
VDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHAN
KREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWT
DEESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGS
NPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAK
PVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDE
APSNVAQAVIEARGK

Sequences:

>Translated_655_residues
MDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQ
ANKYKVPRIAFVNKMDRVGANFLKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPA
DMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSP
VDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHAN
KREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWT
DEESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGS
NPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAK
PVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDE
APSNVAQAVIEARGK
>Mature_655_residues
MDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWRQ
ANKYKVPRIAFVNKMDRVGANFLKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPA
DMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSP
VDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHAN
KREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWT
DEESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGS
NPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAK
PVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDE
APSNVAQAVIEARGK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=665, Percent_Identity=43.4586466165414, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI19923640, Length=672, Percent_Identity=36.3095238095238, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI25306287, Length=672, Percent_Identity=34.5238095238095, Blast_Score=357, Evalue=3e-98,
Organism=Homo sapiens, GI25306283, Length=411, Percent_Identity=37.712895377129, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI4503483, Length=513, Percent_Identity=26.3157894736842, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=98, Percent_Identity=42.8571428571429, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI157426893, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=8e-14,
Organism=Escherichia coli, GI1789738, Length=655, Percent_Identity=99.8473282442748, Blast_Score=1346, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=445, Percent_Identity=25.1685393258427, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI48994988, Length=302, Percent_Identity=27.8145695364238, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788922, Length=116, Percent_Identity=35.3448275862069, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17533571, Length=662, Percent_Identity=41.8429003021148, Blast_Score=479, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17556745, Length=667, Percent_Identity=28.1859070464768, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17506493, Length=426, Percent_Identity=26.9953051643192, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=117, Percent_Identity=38.4615384615385, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=651, Percent_Identity=41.7818740399386, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322359, Length=753, Percent_Identity=28.8180610889774, Blast_Score=310, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6324707, Length=480, Percent_Identity=25.625, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320593, Length=480, Percent_Identity=25.625, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=133, Percent_Identity=35.3383458646617, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582462, Length=657, Percent_Identity=43.6834094368341, Blast_Score=536, Evalue=1e-152,
Organism=Drosophila melanogaster, GI221458488, Length=700, Percent_Identity=30.4285714285714, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24585711, Length=771, Percent_Identity=27.1076523994812, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24585713, Length=771, Percent_Identity=27.1076523994812, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24585709, Length=771, Percent_Identity=27.1076523994812, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI78706572, Length=142, Percent_Identity=33.0985915492958, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574573, Length=101, Percent_Identity=38.6138613861386, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 72241; Mature: 72241

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGA
CCCHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHHHHHHH
VMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKTRLGANPVPL
HEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
QLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAEA
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
SEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCHHHHHHHHHHHCCCC
VDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTV
CCCCHHCCCCCCCCCCCHHHCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHCCCHHH
LNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERM
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCEEEHHH
EFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDII
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCHHHHHHH
VDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEEECCCCC
NPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSS
CCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCEEEEECCCCCCCCH
ELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEV
HHHHHHHHHHHHHHHHHHCCCHHHCCHHEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCC
TGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEARGK
CEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERSMRVLDGA
CCCHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECHHHHHHHHH
VMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKTRLGANPVPL
HEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
QLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAEA
EEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
SEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCHHHHHHHHHHHCCCC
VDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTV
CCCCHHCCCCCCCCCCCHHHCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHCCCHHH
LNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDPDAPIILERM
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCEEEHHH
EFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDII
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCHHHHHHH
VDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPGS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEEEEECCCCC
NPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSS
CCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCEEEEECCCCCCCCH
ELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEV
HHHHHHHHHHHHHHHHHHCCCHHHCCHHEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCC
TGVKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEARGK
CEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA