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The map label for this gene is insK [H]

Identifier: 30065164

GI number: 30065164

Start: 4082600

End: 4083451

Strand: Direct

Name: insK [H]

Synonym: S4178

Alternate gene names: 30065164

Gene position: 4082600-4083451 (Clockwise)

Preceding gene: 30065157

Following gene: 30065167

Centisome position: 88.76

GC content: 42.96

Gene sequence:

>852_bases
GTGAAAGTACTCAACGAGCTAAGGCAGTTTTATCCTCTTGATGAGCTTCTCAGGGCTGCGGAGATACCGCGCAGTACGTT
TTATTACCATCTAAAGGCTCTCAGCAAGCCTGACAAGTATGCGGACGTTAAAAAGCGTATTGGTGAGATTTATCACGAGA
ATAGAGGCCGATACGGATACCGTAGGGTAACGCTGTCTCTTCATCGAGAAGGGAAACAGATTAACCATAAAGCTGTTCAG
CGCCTGATGGGAACCCTCTCACTTAAAGCAGCGATTAAGGTCAAGCGATACCGCTCTTACAGAGGAGAGGTAGGGCAAAC
CGCCCCTAATGTTCTCCAAAGAGATTTCAAGGCTACGCGGCCAAACGAGAAGTGGGTTACCGATGTTACTGAATTTGCAG
TCAATGGGCGCAAGCTGTATTTGTCTCCAGTAATAGATCTCTTCAACAACGAAGTTATTTCTTACAGCCTTTCGGAAAGA
CCAGTGATGAACATGGTTGAGAATATGCTCGATCAGGCATTCAAAAAGCTTAATCCTCACGAGCATCCTGTTCTGCACTC
TGACCAGGGATGGCAGTATCGTATGAGAAGATATCAAAATATCCTTAAAGAACATGGTATTAAACAAAGCATGTCCAGAA
AAGGCAATTGTCTGGATAATGCTGTGGTGGAGTGTTTCTTTGGAACCTTAAAGTCGGAGTGTTTTTATCTTGATGAGTTC
AGTAATATAAGCGAACTGAAGGATGCTGTTACGGAATATATTGAATACTATAACAGCAGAAGAATTAGCCTGAAATTAAA
AGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTATAA

Upstream 100 bases:

>100_bases
AAGACCGACGCATTGAGGATCTTGAAAGGCAAGTTCGATTTCTTGAAACGCGGCTTATGTATCTAAAAAAGCTGAAAGCC
TTAGCTCATCCCACGAAAAA

Downstream 100 bases:

>100_bases
CTGTCCAACTTTTTGGGGTCAGTACAAAGTGAGGCTACCCTATGCCTGAACACCATAAGGTTAGCCTCTTACTCGTTGGA
AATCAACACAGGGGGCTGGG

Product: IS150 putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQ
RLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV

Sequences:

>Translated_283_residues
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQ
RLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV
>Mature_283_residues
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQ
RLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSER
PVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=231, Percent_Identity=31.6017316017316, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1789981, Length=283, Percent_Identity=99.6466431095406, Blast_Score=589, Evalue=1e-170,
Organism=Escherichia coli, GI1788405, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787263, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786753, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786570, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786490, Length=273, Percent_Identity=30.7692307692308, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33448; Mature: 33448

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRIGEIYHENRGRYGY
CHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCH
RRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATR
HEEEHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPH
CCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
EHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEHHHH
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCC
>Mature Secondary Structure
MKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRIGEIYHENRGRYGY
CHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCH
RRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRDFKATR
HEEEHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFKKLNPH
CCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
EHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEF
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEHHHH
SNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]