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The map label for this gene is spoU

Identifier: 30065073

GI number: 30065073

Start: 3979737

End: 3980426

Strand: Reverse

Name: spoU

Synonym: S4078

Alternate gene names: 30065073

Gene position: 3980426-3979737 (Counterclockwise)

Preceding gene: 30065074

Following gene: 30065072

Centisome position: 86.54

GC content: 55.22

Gene sequence:

>690_bases
ATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGT
CCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTG
GTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGC
GATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAATGCTGTCGATTTCCGCGA
AATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGG
ATCAGGACATCATCATTCCGATGATCGGCATGGTGCAGTCGCTGAATGTTTCCGTTGCCTCAGCCCTCATTCTTTACGAA
GCCCAGCGTCAGCGGCAAAATGCAGGCATGTACCTGCGTGAAAACAGCATGTTGCCGGAAGCAGAGCAACAACGCCTGTT
GTTTGAAGGCGGCTATCCGGTGCTGGCGAAAGTCGCAAAACGCAAAGGCCTGCCTTATCCCCACGTCAATCAGCAAGGCG
AGATCGAAGCTGATGCCGACTGGTGGGCTACTATGCAGGCTGCAGGGTAA

Upstream 100 bases:

>100_bases
TCTGACCGCTCGTGACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCC
GAAACCGAAATTAATGTTTT

Downstream 100 bases:

>100_bases
GTGCCATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACTTAGTAACAAACTG
GCGAAAATCAACCTGCATAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG

Sequences:

>Translated_229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG
>Mature_229_residues
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIG
DAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG

Specific function: Specifically methylates guanosine-18 in various tRNAs

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Escherichia coli, GI1790083, Length=229, Percent_Identity=100, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1790623, Length=160, Percent_Identity=31.875, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMH_ECO57 (P0AGJ3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F91194
- PIR:   G86041
- RefSeq:   NP_290231.1
- RefSeq:   NP_312553.1
- ProteinModelPortal:   P0AGJ3
- SMR:   P0AGJ3
- EnsemblBacteria:   EBESCT00000028477
- EnsemblBacteria:   EBESCT00000060439
- GeneID:   915517
- GeneID:   960894
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5077
- KEGG:   ecs:ECs4526
- GeneTree:   EBGT00050000008907
- HOGENOM:   HBG309728
- OMA:   VLGAEKW
- ProtClustDB:   PRK11081
- BioCyc:   ECOL83334:ECS4526-MONOMER
- GO:   GO:0005737
- InterPro:   IPR022724
- InterPro:   IPR001537

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase

EC number: =2.1.1.34

Molecular weight: Translated: 25343; Mature: 25343

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: BINDING 119-119 BINDING 139-139 BINDING 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEECCCCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCEEEEEEC
EKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
AEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG
HHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHEEEECCC
>Mature Secondary Structure
MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTM
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEECCCCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCCCEEEEEEC
EKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
AEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEADADWWATMQAAG
HHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796