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The map label for this gene is dnaA

Identifier: 30065010

GI number: 30065010

Start: 3904162

End: 3905565

Strand: Reverse

Name: dnaA

Synonym: S4009

Alternate gene names: 30065010

Gene position: 3905565-3904162 (Counterclockwise)

Preceding gene: 30065015

Following gene: 30065009

Centisome position: 84.92

GC content: 53.63

Gene sequence:

>1404_bases
GTGTCACTTTCGCTTTGGCAGCAGTGTCTTGCCCGATTGCAGGATGAGTTACCAGCCACAGAATTCAGTATGTGGATACG
CCCATTGCAGGCGGAACTGAGCGATAACACGCTGGCCCTGTACGCGCCAAACCGTTTTGTCCTCGATTGGGTACGGGACA
AGTACCTTAATAATATCAATGGACTGCTAACCAGTTTCTGCGGAGCGGATGCCCCACAGCTGCGTTTTGAAGTCGGCACC
AAACCGGTGACGCAAACGCCACAAGCGGCAGTGACGAGCAACGTCGCGGCCCCTGCACAGGTGGCGCAAACGCAGCCGCA
ACGTGCTGCGCCTTCTACGCGCTCAGGTTGGGATAACGTCCCGGCCCCGGCAGAACCGACCTATCGTTCTAACGTAAACG
TCAAACACACGTTTGATAACTTCGTTGAAGGTAAATCTAACCAACTGGCGCGCGCGGCGGCTTGCCAGGTGGCGGATAAC
CCTGGCGGTGCCTATAACCCGTTGTTCCTTTATGGCGGCACGGGTCTGGGTAAAACTCACCTGCTGCATGCGGTGGGTAA
CGGCATTATGGCGCGCAAGCCGAATGCCAAAGTGGTTTATATGCACTCCGAGCGCTTTGTTCAGGACATGGTTAAAGCCC
TGCAAAACAACGCGATCGAAGAGTTTAAACGCTACTACCGTTCCGTAGATGCACTGCTGATCGACGATATTCAGTTTTTT
GCTAATAAAGAACGATCTCAGGAAGAGTTTTTCCACACCTTCAACGCCCTGCTGGAAGGTAATCAACAGATCATTCTCAC
CTCGGATCGCTATCCGAAAGAGATCAACGGCGTTGAGGATCGTTTGAAATCCCGCTTCGGTTGGGGACTGACTGTGGCGA
TCGAACCGCCAGAGCTGGAAACCCGTGTGGCGATCCTGATGAAAAAGGCCGACGAAAATGACATTCGTTTGCCGGGTGAA
GTGGCGTTCTTTATCGCCAAGCGTCTACGATCTAACGTACGTGAGCTGGAAGGGGCGCTGAACCGCGTTATTGCTAACGC
CAACTTTACCGGACGGGCGATCACCATCGACTTCGTGCGTGAGGCGCTGCGCGACTTGCTGGCATTGCAGGAAAAACTGG
TCACCATCGACAATATTCAGAAGACGGTGGCGGAGTACTACAAGATCAAAGTCGCGGATCTCCTTTCCAAGCGTCGATCC
CGCTCGGTGGCGCGTCCGCGCCAGATGGCGATGGCGCTAGCGAAAGAGCTGACTAACCACAGTCTGCCAGAGATTGGCGA
TGCGTTTGGTGGGCGTGACCACACGACGGTGCTTCATGCCTGCCGTAAGATCGAGCAGTTGCGTGAAGAGAGCCACGATA
TCAAAGAAGATTTTTCAAATTTAATCAGAACATTGTCATCGTAA

Upstream 100 bases:

>100_bases
TTCGTGCGTCACCCTCAAGCAGGGTCTTTTCGACGTACGTCAACAATCATGAATGTTTCAGCCTTAGTCATTATCGACTT
TTGTTCGAGTGGAGTCCGCC

Downstream 100 bases:

>100_bases
ACCTATGAAATTTACCGTAGAACGTGAGCATTTATTAAAACCGCTACAACAGGTGAGCGGTCCGTTAGGTGGTCGTCCTA
CGCTACCGATTCTCGGTAAT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGT
KPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFF
ANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGE
VAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRS
RSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS

Sequences:

>Translated_467_residues
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGT
KPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFF
ANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGE
VAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRS
RSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
>Mature_466_residues
SLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTK
PVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADNP
GGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFFA
NKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEV
AFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSR
SVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=467, Percent_Identity=99.7858672376874, Blast_Score=964, Evalue=0.0,
Organism=Escherichia coli, GI226510964, Length=245, Percent_Identity=26.9387755102041, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52498; Mature: 52367

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNIN
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
GLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNV
HHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCHHCCCCCCCCCCCC
PAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADNPGGAYNPLFLYGGTGLGKTH
CCCCCCCCCCCCCEEECHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
LLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFF
HHHHHCCCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELE
HCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHCCCCEEEEECCCCHH
TRVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHH
EALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
SLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNIN
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
GLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNV
HHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCHHCCCCCCCCCCCC
PAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADNPGGAYNPLFLYGGTGLGKTH
CCCCCCCCCCCCCEEECHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
LLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFF
HHHHHCCCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELE
HCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCCHHHHHHHHCCCCEEEEECCCCHH
TRVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHH
EALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
SLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA