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The map label for this gene is ilvB

Identifier: 30064984

GI number: 30064984

Start: 3872959

End: 3874647

Strand: Reverse

Name: ilvB

Synonym: S3978

Alternate gene names: 30064984

Gene position: 3874647-3872959 (Counterclockwise)

Preceding gene: 30064985

Following gene: 30064983

Centisome position: 84.24

GC content: 56.07

Gene sequence:

>1689_bases
ATGGCAAGTTCGGGCACAACATCGACGCGTAAGCGCTTTACCGGCGCAGAATTTATCGTTCATTTCCTGGAACAGCAGGG
CATTAAGATTGTGACGGGCATTCCGGGCGGTTCTATCCTGCCTGTTTACGACGCCTTAAGCCAAAGCACGCAAATCCGCC
ATATTCTGGCTCGCCATGAACAGGGCGCGGGGTTTATCGCTCAGGGAATGGCGCGCACCGACGGTAAACCGGCGGTCTGT
ATGGCCTGTAGCGGGCCGGGTGCGACTAACCTGGTGACCGCCATTGCTGATGCGCGGCTGGACTCCATCCCGCTGATTTG
CATCACTGGTCAGGTTCCCGCCTCGATGATCGGCACCGACGCCTTCCAAGAAGTGGACACCTACGGCATCTCTATCCCCA
TCACCAAACACAACTATCTGGTCAGACATATCGAAGAGCTCCCACAGGTCATGAGCGATGCCTTCCGCATTGCGCAATCA
GGCCGCCCTAGCCCGGTGTGGATAGACATTCCTAAGGATGTACAAACGGCGGTTTTTGAGATTGAAGCTCAGCCCGCGGT
GGCAGAAAAAGCCGTTGCACCCGCCTTTAGCGAAGAAAGCATTCGTGACGCAGCTACAATGATTAACGCTGCCAAACGCC
CGGTGCTTTATCTGGGTGGTGGTGTGATCAATGCGCCTGCGCGGGTGCGTGAACTGGCGGAGAAAGCGCAACTGCCTACC
ACCATGACTTTAATGGCGCTGGGCATGCTGCCAAAAGCGCATCCGTTGTCGCTGGGTATGCTGGGGATGCACGGCGTGCG
CAGTACCAACTATATTTTGCAGGAGGCGGATTTGTTGATTGTGCTCGGTGCGCGTTTTGATGACCGGGCGATTGGCAAAA
CCGAGCAGTTCTGTCCGAATGCCAAAATTATTCATGTCGATATCGATCGCGCAGAGCTGGGTAAAATCAAGCAGCCGCAT
GTGGCGATTCAGGCGGATGTTGATGACGTGCTGGCGCAATTGATCCCGCGGGTGGAAGCGCAACCGCGTGCAGAGTGGCA
CCAGTTGGTAGCGGATTTGCAGCGCGAATTCCCGTGTCCAATCCCGAAAGCGTGCGATCCATTAAGCCATTACGGCCTGA
TCAACGCCGTTGCCGCCTGTGTCGATGACAATGCGATTATCACCACCGATGTGGGTCAGCATCAGATGTGGACCGCGCAA
GCTTATCCGCTCAATCGCCCACGCCAGTGGCTGACCTCCGGTGGGCTGGGCACGATGGGTTTTGGCCTGCCTGCGGCGAT
TGGCGCGGCGCTGGCGAACCCGGATCGCAAAGTGTTGTGTTTCTCCGGCGACGGCAGCCTGATGATGAATATTCAGGAGA
TGGCGACCGCCAGTGAAAATCAGCTGGATGTCAAAATCATTCTGATGAACAACGAAGCGCTGGGGCTGGTGCATCAGCAA
CAGAGTCTGTTCTACGAGCAAGGCGTTTTTGCCGCCACCTATCCGGGCAAAATCAACTTTATGCAGATTGCCGCCGGATT
CGGCCTCGAAACCTGTGATTTGAATAACGAAGCCGATCCGCAGGCTGCATTGCAGGAAATCATCAATCGCCCTGGCCCGG
CGCTGATCCATGTGCGCATTGATGCCGAAGAAAAAGTTTACCCGATGGTGCCGCCAGGTGCGGCGAATACTGAAATGGTG
GGGGAATAA

Upstream 100 bases:

>100_bases
GGAACAACACACGATTCCAAAACCCCGCCGGCGCAAACCGGGCGGGGTTTTTCGTTTAAGCACCTCCCGGAAAGTCGGCC
CAGAAGAAAAGGACTGGAGC

Downstream 100 bases:

>100_bases
GCCATGCAAAACACAACTCATAACAACGTAATTCTGGAGCTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTG
TGGCCTTTTTGCCCGCCGCG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
QSLFYEQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE

Sequences:

>Translated_562_residues
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
QSLFYEQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE
>Mature_561_residues
ASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCM
ACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQSG
RPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYLGGGVINAPARVRELAEKAQLPTT
MTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHV
AIQADVDDVLAQLIPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQA
YPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQQ
SLFYEQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVG
E

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=572, Percent_Identity=26.048951048951, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=25.4937163375224, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=98.7544483985765, Blast_Score=1143, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=39.2920353982301, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=484, Percent_Identity=35.9504132231405, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=28.804347826087, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=26.6247379454927, Blast_Score=142, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=591, Percent_Identity=26.3959390862944, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=24.910394265233, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=24.910394265233, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=40.4513888888889, Blast_Score=432, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=23.2793522267206, Blast_Score=104, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323073, Length=507, Percent_Identity=21.301775147929, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=477, Percent_Identity=28.9308176100629, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60494; Mature: 60363

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHCHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQSGRPSPVWIDIPKDVQTAVFE
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
IEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
EECCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEHHCCCCCCCCCHHEECCCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPRVEAQPRAEWHQLVADLQREFPCP
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
IPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEHHH
QSLFYEQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHCHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQSGRPSPVWIDIPKDVQTAVFE
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEE
IEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
EECCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEHHCCCCCCCCCHHEECCCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPRVEAQPRAEWHQLVADLQREFPCP
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
IPKACDPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEHHH
QSLFYEQGVFAATYPGKINFMQIAAGFGLETCDLNNEADPQAALQEIINRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]