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The map label for this gene is wecC

Identifier: 30064918

GI number: 30064918

Start: 3792207

End: 3793469

Strand: Reverse

Name: wecC

Synonym: S3899

Alternate gene names: 30064918

Gene position: 3793469-3792207 (Counterclockwise)

Preceding gene: 30064919

Following gene: 30064917

Centisome position: 82.48

GC content: 53.76

Gene sequence:

>1263_bases
ATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTACATCGGGCTGCCTACAGCAGCAGCGTTTGCCTCACGGCAAAA
ACAGGTGATTGGTGTCGATATCAACCAACACGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAGCCTGATT
TGGCGAGCGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTG
ATTGCTGTACCTACGCCGTTTAAAGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCC
AGTGCTGAAAAAAGGCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAG
CAGAGATGCGCCCGGATCTGACTTTCCCACAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGT
GTATTACCGGGCCAGGTTATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCG
CGCCAGCGAATTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCA
CCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTC
TGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCAT
TGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTTAACGATC
ACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTTGCCGACTGCCTTGCTGCTACCGATAAACGAGCCAGTGAA
CTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGA
ACTGATCGCCCAGTGGCACAGCGGCGAAACGCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGC
TTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAA
GTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGA

Upstream 100 bases:

>100_bases
AAAACGAATATCAAGCTATGAGCCGCGCCCATAACCCGTATGGTGATGGTCAGGCATGCTCTCGCATTCTGGAAGCGTTA
AAAAATAATCGGATATCACT

Downstream 100 bases:

>100_bases
TGAGAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGAT
GCGGTGGTAGTGGTCGATAA

Product: UDP-N-acetyl-D-mannosamine dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWL
IAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASE
LKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFK
VINGDNVHQQYVVDAKGVWR

Sequences:

>Translated_420_residues
MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWL
IAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASE
LKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFK
VINGDNVHQQYVVDAKGVWR
>Mature_419_residues
SFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLI
AVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPERV
LPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINVW
ELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASEL
KIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFKV
INGDNVHQQYVVDAKGVWR

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA)

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family

Homologues:

Organism=Homo sapiens, GI4507813, Length=457, Percent_Identity=26.6958424507659, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI296040438, Length=358, Percent_Identity=25.1396648044693, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI48994968, Length=420, Percent_Identity=100, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1788340, Length=376, Percent_Identity=23.6702127659574, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17560350, Length=437, Percent_Identity=28.8329519450801, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17136908, Length=370, Percent_Identity=27.5675675675676, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECC_ECO57 (P67066)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B86065
- PIR:   H91218
- RefSeq:   NP_290418.1
- RefSeq:   NP_312747.1
- ProteinModelPortal:   P67066
- SMR:   P67066
- EnsemblBacteria:   EBESCT00000023975
- EnsemblBacteria:   EBESCT00000059523
- GeneID:   915235
- GeneID:   960381
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5298
- KEGG:   ecs:ECs4720
- GeneTree:   EBGT00050000010460
- HOGENOM:   HBG400967
- OMA:   REMMATE
- ProtClustDB:   PRK11064
- BioCyc:   ECOL83334:ECS4720-MONOMER
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11374
- PIRSF:   PIRSF000124
- TIGRFAMs:   TIGR03026

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N; SSF48179 6DGDH_C_like; SSF52413 UDP-Glc/GDP-Man_DH_C

EC number: 1.1.1.- [C]

Molecular weight: Translated: 45839; Mature: 45708

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00678 WD_REPEATS_1

Important sites: ACT_SITE 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKT
CCEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHCCCCCCEEEEECCHHHHHHHH
AVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILEST
HHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
SPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPERVLPGQVMVELIKNDRVIGGM
CCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEECCC
TPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
CCHHHHHHHHEEEEEECCEEEEECCCHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCH
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVI
HHHHHHHCCCCCEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEH
DQVKAAVADCLAATDKRASELKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVV
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
EPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFKVINGDNVHQQYVVDAKGVWR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKT
CEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHCCCCCCEEEEECCHHHHHHHH
AVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILEST
HHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
SPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPERVLPGQVMVELIKNDRVIGGM
CCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEECCC
TPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
CCHHHHHHHHEEEEEECCEEEEECCCHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCH
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVI
HHHHHHHCCCCCEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEH
DQVKAAVADCLAATDKRASELKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVV
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
EPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFKVINGDNVHQQYVVDAKGVWR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796