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The map label for this gene is phnE

Identifier: 30064673

GI number: 30064673

Start: 3487169

End: 3487393

Strand: Reverse

Name: phnE

Synonym: S3611

Alternate gene names: 30064673

Gene position: 3487393-3487169 (Counterclockwise)

Preceding gene: 30064674

Following gene: 30064670

Centisome position: 75.82

GC content: 58.67

Gene sequence:

>225_bases
ATGCCGGATGCGGTGCAAGCACCTTATCCGGCCTACAGACCGGAGCCAAACATGCAAACCATCACCATCGCCCCACCCAA
GCGTAGCTGGTTCTCGCTTCTGAGCTGGGCCGTTGTTCTCGACGTGCTGGTCGTCTCGTGGCAGGGCGCGGAAATGGCCC
CGCTTACGCTGATTAAAGACGGCGGCAACATGGCAACCTTCGCGGTAATGACTCCAACTTACTGA

Upstream 100 bases:

>100_bases
CAACGCGTTAAGCGCGATGAGTTCGGTGAGTAAAGCGGTGCAGTAAATCGTAGGTCGGATAAGACGCCCCGGCGTCGCAT
CCGACAATGTGTAGGCGTTG

Downstream 100 bases:

>100_bases
TAGTGTTTTATGTTCAGATAATGCCCGATGACCTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGTGACTTCCGTAC
CAGCCTTGCCAGATGTTGTC

Product: membrane channel protein component of phosphonate transporter

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 74; Mature: 73

Protein sequence:

>74_residues
MPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDGGNMATFAVMTPTY

Sequences:

>Translated_74_residues
MPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDGGNMATFAVMTPTY
>Mature_73_residues
PDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDGGNMATFAVMTPTY

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 8154; Mature: 8022

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKD
CCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
GGNMATFAVMTPTY
CCCEEEEEEECCCC
>Mature Secondary Structure 
PDAVQAPYPAYRPEPNMQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKD
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
GGNMATFAVMTPTY
CCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]