Click here to switch to the map view.

The map label for this gene is trkA

Identifier: 30064611

GI number: 30064611

Start: 3409518

End: 3410894

Strand: Direct

Name: trkA

Synonym: S3546

Alternate gene names: 30064611

Gene position: 3409518-3410894 (Clockwise)

Preceding gene: 30064610

Following gene: 30064612

Centisome position: 74.13

GC content: 49.89

Gene sequence:

>1377_bases
ATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTAC
TGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTGCAGGATAAATTTGACCTGCGAGTCGTGCAGGGGCATGGCTCTC
ATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATG
GTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGCATCCGCTCACCAGACTACGTACGCGA
TGCCGATAAGCTATTTCATTCAGATGCTGTACCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTT
ACCGACTGATTGAGTATCCAGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGTCAGCCTCGCTGTAGTTAAAGCC
TATTATGGCGGCCCGCTGATTGGTAATGCACTTTCGACCATGCGCGAACATATGCCACATATCGATACTCGTGTGGCAGC
AATTTTCCGCCACGATCGCCCCATTCGTCCACAAGGTTCGACCATTGTTGAAGCTGGTGATGAAGTGTTCTTTATTGCCG
CTTCACAGCATATCCGCGCGGTGATGAGTGAATTACAGCGACTGGAAAAACCGTATAAACGGATCATGCTGGTTGGTGGC
GGTAATATCGGTGCAGGGCTGGCGCGTCGTCTGGAAAAAGATTACAGCGTTAAACTCATCGAACGTAATCAGCAGCGCGC
TGCCGAACTGGCGGAAAAGTTGCAGAATACGATCGTCTTTTTTGGTGATGCGTCGGATCAAGAACTGCTGGCCGAAGAAC
ATATCGATCAAGTTGATCTGTTTATTGCCGTCACCAACGATGACGAGGCCAATATCATGTCCGCCATGCTTGCCAAACGT
ATGGGTGCGAAAAAGGTGATGGTATTGATCCAGCGTCGCGCTTATGTGGATCTGGTTCAGGGGAGCGTGATCGATATTGC
AATTTCACCACAACAAGCAACTATTTCTGCGTTGCTTAGCCATGTGCGAAAAGCAGATATTGTTGGTGTTTCCTCATTGC
GCCGCGGCGTAGCAGAAGCTATTGAAGCCGTTGCTCACGGTGATGAAAGCACCTCACGCGTTGTCGGCAGAGTCATTGAC
GAAATCAAGCTACCGCCAGGAACGATTATTGGAGCGGTGGTACGTGGAAACGACGTGATGATTGCCAATGACAATCTGCG
CATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAA
GCCCTTTCTTCTTGTAA

Upstream 100 bases:

>100_bases
AGAGCAACCGGGTAAACAAAATCAGCCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGAT
GAGATAACGGGTCGCGACTG

Downstream 100 bases:

>100_bases
TTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATG
GCTTAACATTTGTTAGACGT

Product: potassium transporter peripheral membrane component

Products: NA

Alternate protein names: K(+)-uptake protein trkA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFDLRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNM
VACQVAYSLFNTPNRIARIRSPDYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHIRAVMSELQRLEKPYKRIMLVGG
GNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQNTIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKR
MGAKKVMVLIQRRAYVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTSRVVGRVID
EIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERLFQPSPFFL

Sequences:

>Translated_458_residues
MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFDLRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNM
VACQVAYSLFNTPNRIARIRSPDYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHIRAVMSELQRLEKPYKRIMLVGG
GNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQNTIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKR
MGAKKVMVLIQRRAYVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTSRVVGRVID
EIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERLFQPSPFFL
>Mature_458_residues
MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFDLRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNM
VACQVAYSLFNTPNRIARIRSPDYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHIRAVMSELQRLEKPYKRIMLVGG
GNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQNTIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKR
MGAKKVMVLIQRRAYVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTSRVVGRVID
EIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERLFQPSPFFL

Specific function: Part of the constitutive potassium transport systems trkG and trkH. May regulate the transport activity of trkG and trkH systems. Binds to NAD+ and NADH

COG id: COG0569

COG function: function code P; K+ transport systems, NAD-binding component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Note=Peripherally bound to the inner side of the inner membrane via the trkG and trkH proteins (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 RCK N-terminal domains

Homologues:

Organism=Escherichia coli, GI1789685, Length=458, Percent_Identity=100, Blast_Score=925, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRKA_ECO57 (P0AGJ0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91148
- PIR:   G85993
- RefSeq:   NP_289851.1
- RefSeq:   NP_312182.1
- ProteinModelPortal:   P0AGJ0
- EnsemblBacteria:   EBESCT00000026224
- EnsemblBacteria:   EBESCT00000059510
- GeneID:   915986
- GeneID:   961474
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4660
- KEGG:   ecs:ECs4155
- GeneTree:   EBGT00050000011566
- HOGENOM:   HBG697234
- OMA:   HTPTKIA
- ProtClustDB:   PRK09496
- BioCyc:   ECOL83334:ECS4155-MONOMER
- GO:   GO:0005488
- InterPro:   IPR006036
- InterPro:   IPR016040
- InterPro:   IPR006037
- InterPro:   IPR003148
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00335

Pfam domain/function: PF02080 TrkA_C; PF02254 TrkA_N

EC number: NA

Molecular weight: Translated: 50369; Mature: 50369

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS51202 RCK_C; PS51201 RCK_N

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFDLRVVQGHGSHPRVLRE
CEEEEEECCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCEEEEECCCCCCHHHHH
AGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSPDYVRDADKLFHSDAVPID
CCCCCCCEEEEEECCCCCCEEHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHCCCCCCHH
HLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKAYYGGPLIGNALSTMREHMPH
HHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEHHCCCHHHHHHHHHHHHCCC
IDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHIRAVMSELQRLEKPYKRIMLVGG
HHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHEEEEEEC
GNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQNTIVFFGDASDQELLAEEHIDQVDL
CCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCEEEE
FIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRAYVDLVQGSVIDIAISPQQATISALLS
EEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
HVRKADIVGVSSLRRGVAEAIEAVAHGDESTSRVVGRVIDEIKLPPGTIIGAVVRGNDVM
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEE
IANDNLRIEQGDHVIMFLTDKKFITDVERLFQPSPFFL
EECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFDLRVVQGHGSHPRVLRE
CEEEEEECCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCEEEEECCCCCCHHHHH
AGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSPDYVRDADKLFHSDAVPID
CCCCCCCEEEEEECCCCCCEEHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHCCCCCCHH
HLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKAYYGGPLIGNALSTMREHMPH
HHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEHHCCCHHHHHHHHHHHHCCC
IDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHIRAVMSELQRLEKPYKRIMLVGG
HHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHEEEEEEC
GNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQNTIVFFGDASDQELLAEEHIDQVDL
CCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHCCCEEEE
FIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRAYVDLVQGSVIDIAISPQQATISALLS
EEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHH
HVRKADIVGVSSLRRGVAEAIEAVAHGDESTSRVVGRVIDEIKLPPGTIIGAVVRGNDVM
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCEEE
IANDNLRIEQGDHVIMFLTDKKFITDVERLFQPSPFFL
EECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796