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The map label for this gene is sspA

Identifier: 30064562

GI number: 30064562

Start: 3354737

End: 3355375

Strand: Reverse

Name: sspA

Synonym: S3484

Alternate gene names: 30064562

Gene position: 3355375-3354737 (Counterclockwise)

Preceding gene: 30064563

Following gene: 30064561

Centisome position: 72.95

GC content: 53.68

Gene sequence:

>639_bases
ATGGCTGTCGCTGCCAACAAACGTTCGGTAATGACGCTGTTTTCCGGTCCTACTGACATCTATAGCCATCAGGTCCGCAT
TGTGCTGGCTGAGAAAGGTGTAAGTTTCGAGATCGAACACGTGGAAAAGGACAATCCGCCTCAGGATCTGATTGACCTCA
ACCCGAATCAGAGCGTTCCGACCCTGGTGGATCGTGAGCTGACCCTGTGGGAATCTCGCATCATTATGGAATATCTGGAT
GAGCGTTTCCCGCATCCGCCACTGATGCCTGTTTACCCGGTAGCTCGCGGTGAAAGCCGTCTGTACATGCATCGCATCGA
AAAAGACTGGTACACGCTGATGAACACTATCATCAACGGTTCAGCTTCTGAAGCAGATGCCGCACGTAAGCAACTGCGCG
AAGAACTGCTGGCGATTGCGCCGGTCTTCGGTCAGAAGCCGTACTTCCTGAGCGATGAGTTCAGCCTGGTCGATTGCTAC
CTTGCTCCGCTGCTGTGGCGTCTGCCGCAACTGGGCATCGAGTTCAGCGGCCCGGGTGCGAAAGAGCTGAAAGGCTATAT
GACCCGCGTCTTTGAGCGTGACTCTTTCCTTGCTTCTTTAACTGAAGCAGAACGTGAAATGCGTCTGGGCCGGAGTTAA

Upstream 100 bases:

>100_bases
TTCGAACAAAGAGAGTCGTTCCTTATTGGGTAACACAACTTCTGACTGGCCACCTGGTGGCTGGTAGCAGTAAAAATTCT
GACTATACCTGGAGGTTTTC

Downstream 100 bases:

>100_bases
TCTGTATGGATTTGTCACAGCTAACACCACGTCGTCCCTATCTGCTGCGTGCATTCTATGAGTGGTTGCTGGATAACCAG
CTCACGCCGCACCTGGTGGT

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLD
ERFPHPPLMPVYPVARGESRLYMHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS

Sequences:

>Translated_212_residues
MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLD
ERFPHPPLMPVYPVARGESRLYMHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS
>Mature_211_residues
AVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDE
RFPHPPLMPVYPVARGESRLYMHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCYL
APLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain

Homologues:

Organism=Escherichia coli, GI1789624, Length=212, Percent_Identity=100, Blast_Score=437, Evalue=1e-124,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SSPA_ECO57 (P0ACA5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85987
- PIR:   F91141
- RefSeq:   NP_289797.1
- RefSeq:   NP_312129.1
- ProteinModelPortal:   P0ACA5
- SMR:   P0ACA5
- MINT:   MINT-1228551
- EnsemblBacteria:   EBESCT00000025707
- EnsemblBacteria:   EBESCT00000057385
- GeneID:   916049
- GeneID:   959207
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4587
- KEGG:   ecs:ECs4102
- GeneTree:   EBGT00050000009154
- HOGENOM:   HBG289890
- OMA:   MERQFAR
- ProtClustDB:   PRK09481
- BioCyc:   ECOL83334:ECS4102-MONOMER
- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:1.20.1050.10
- Gene3D:   G3DSA:3.40.30.10

Pfam domain/function: PF00043 GST_C; PF02798 GST_N; SSF47616 GST_C_like; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 24305; Mature: 24174

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50405 GST_CTER; PS50404 GST_NTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVP
CCCCCCCCCEEEEECCCHHHHCCEEEEEEECCCCEEEEEECCCCCCCHHHEECCCCCCCC
TLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLYMHRIEKDWYTLMNTIING
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC
SASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCYLAPLLWRLPQLGIEFSGPGA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCCEEECCCCH
KELKGYMTRVFERDSFLASLTEAEREMRLGRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKDNPPQDLIDLNPNQSVP
CCCCCCCCEEEEECCCHHHHCCEEEEEEECCCCEEEEEECCCCCCCHHHEECCCCCCCC
TLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLYMHRIEKDWYTLMNTIING
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCC
SASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCYLAPLLWRLPQLGIEFSGPGA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCCEEECCCCH
KELKGYMTRVFERDSFLASLTEAEREMRLGRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796