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The map label for this gene is dacB

Identifier: 30064520

GI number: 30064520

Start: 3312263

End: 3313696

Strand: Direct

Name: dacB

Synonym: S3440

Alternate gene names: 30064520

Gene position: 3312263-3313696 (Clockwise)

Preceding gene: 30064518

Following gene: 30064525

Centisome position: 72.02

GC content: 53.14

Gene sequence:

>1434_bases
ATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCGAATGTTGATGAGTACGT
TACTCAACTCCCCGCAGGTGCCAACCTTGCCCTGATGGTGCAAAAAGTCGGTGCGTCGGCCCCCGCTATTGATTACCACA
GTCAGCAGATGGCGCTGCCCGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTT
CGTTTCACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGTACTTAAGGGTGACTTAGTGGCGCGATTTGGTGC
CGATCCGACGTTAAAACGTCAGGATATTCGCAATATGGTCGCGACTTTGAAAAAATCTGGCGTCAACCAAATCGATGGCA
ATGTGTTGATAGATACCTCCATTTTCGCCAGCCACGATAAAGCCCCCGGCTGGCCATGGAATGACATGACACAATGCTTT
AGCGCTCCGCCTGCTGCCGCCATAGTTGACCGCAACTGTTTCTCCGTCTCGCTCTACAGTGCCCCAAAGCCTGGTGATAT
GGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAG
CGCAATACTGCGAACTGGATGTGGTGCCAAGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAG
CCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGG
TATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCC
CGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATCGGCCAT
GCGCGCTTCAATGTGCCTGGAACATGGCGGGCTGGGTCGGACGCCGTGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGA
TATTGGTAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGATTGCCCCCGCCACCATGATGCAGGTGC
TGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTTGCGGGCTATGACGGCTCTTTGCAG
TACCGTGCAGGTCTGCATCAGGCGGGCGTGGATGGCAAAGTCTCAGCGAAAACCGGTTCGTTGCAGGGGGTATATAACCT
GGCGGGATTCATTACCACAGCGAGCGGGCAACGAATGGCATTTGTACAATATCTTTCTGGCTATGCAGTAAAACCCGCGG
ATCAGCGTAATCGCCGTATTCCGTTAGTGCGTTTTGAAAGCCGTTTGTACAAAGATATTTATCAGAACAATTAG

Upstream 100 bases:

>100_bases
ATGGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTC
CAGGTTGTTAGCGCGAGATT

Downstream 100 bases:

>100_bases
TCAAAAAGAAACCCCGGCGCATGGCCGGGGTTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTGAA
CGCCTTATCCGGCCTACAAA

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVGASAPAIDYHSQQMALPASTQKVITALAALIQLGPDF
RFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPSDLNRFTLTGCLPQRSE
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGH
ARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQ
YRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAVKPADQRNRRIPLVRFESRLYKDIYQNN

Sequences:

>Translated_477_residues
MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVGASAPAIDYHSQQMALPASTQKVITALAALIQLGPDF
RFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPSDLNRFTLTGCLPQRSE
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGH
ARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQ
YRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAVKPADQRNRRIPLVRFESRLYKDIYQNN
>Mature_477_residues
MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVGASAPAIDYHSQQMALPASTQKVITALAALIQLGPDF
RFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPSDLNRFTLTGCLPQRSE
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGH
ARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQ
YRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAVKPADQRNRRIPLVRFESRLYKDIYQNN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=477, Percent_Identity=99.3710691823899, Blast_Score=981, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51814; Mature: 51814

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVGASAPAIDYHSQQMALP
CCHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCEEHEEHHCCCCCEECCCCCCEECC
ASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMV
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECHHHHHCCCCCCHHHHHHHHHH
ATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCFSAPPAAAIVDRNCFSVSLYS
HHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCHHEECCCCEEEEEEC
APKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPSDLNRFTLTGCLPQRSE
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCC
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLK
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHH
IMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLS
HHHHCCCCHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCC
RHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQYRAGLHQAGVDGKVSAKTGS
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEHHCCCHHCCCCCEEECCCCC
LQGVYNLAGFITTASGQRMAFVQYLSGYAVKPADQRNRRIPLVRFESRLYKDIYQNN
CCHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVGASAPAIDYHSQQMALP
CCHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCEEHEEHHCCCCCEECCCCCCEECC
ASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLVARFGADPTLKRQDIRNMV
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECHHHHHCCCCCCHHHHHHHHHH
ATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCFSAPPAAAIVDRNCFSVSLYS
HHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHCCCCCHHEECCCCEEEEEEC
APKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCELDVVPSDLNRFTLTGCLPQRSE
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCC
PLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLRQTQVNEPGTVVASKQSAPLHDLLK
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHH
IMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSDAVRQILRQQAGVDIGNTIIADGSGLS
HHHHCCCCHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCC
RHNLIAPATMMQVLQYIAQHDNELNFISMLPLAGYDGSLQYRAGLHQAGVDGKVSAKTGS
CCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEHHCCCHHCCCCCEEECCCCC
LQGVYNLAGFITTASGQRMAFVQYLSGYAVKPADQRNRRIPLVRFESRLYKDIYQNN
CCHHHHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]