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The map label for this gene is yhbU

Identifier: 30064498

GI number: 30064498

Start: 3284808

End: 3285803

Strand: Direct

Name: yhbU

Synonym: S3416

Alternate gene names: 30064498

Gene position: 3284808-3285803 (Clockwise)

Preceding gene: 30064495

Following gene: 30064499

Centisome position: 71.42

GC content: 57.23

Gene sequence:

>996_bases
ATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGG
GCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTG
TCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGTGCC
GTGGATATGGCGGCGCAGCTGGGTGCCGACGCGCTGATCCTCGCCGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTA
CCCGCATATTGAACGTCATGTGTCAGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTG
ACGTTGCCCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAGCTGGCACGGGTTACTCCTGTCCCGCTG
GAAGTGTTTGCTTTCGGCAGCCTGTGTATTATGTCGGAAGGCCGTTGCTATCTGTCGTCGTATCTGACGGGTGAGTCGCC
CAACACTGTGGGTGCGTGTTCTCCGGCCCGTTTCGTGCGCTGGCAACAAACGCCGCAGGGGCTGGAATCCCGCCTGAACG
AAGTGCTGATCGACCGTTATCAGGACGGCGAAAACGCAGGTTATCCGACGCTGTGTAAAGGGCGTTATCTGGTGGACGGC
GAGCGCTATCACGCGCTGGAAGAACCAACCAGTCTCAATACCCTGGAACTGCTGCCGGAGTTAATGGCTGCGAATATTGC
TTCGGTGAAAATTGAAGGCCGCCAGCGTAGCCCGGCGTATGTCAGCCAGGCGGCGAAAGTCTGGCGTCAGGCTATCGACC
GTTGTAAGGCCGATCCGCAAAACTTCGTACCGCAAAGCGCGTGGATGGAGACGCTCGGGTCGATGTCCGAAGGCACGCAA
ACCACCCTTGGCGCGTATCACCGTAAATGGCAGTGA

Upstream 100 bases:

>100_bases
CTTTAACTGCCTTAAATCAAAAATTGTCGCAGCAAGGTTAACTAAAATCCCAGTTCGTTAACATTTTTGCGTTTTGATAG
CGCAACCTTCAGGAAAAATT

Downstream 100 bases:

>100_bases
GAAGAGCAATGAAATATTCCTTAGGGCCAGTGCTGTGGTACTGGCCAAAAGAGACGCTGGAAGATTTTTATCAGCAAGCC
GCCGCCAGCAGCGCCGACGT

Product: putative collagenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTEKKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRA
VDMAAQLGADALILADLAMLEYAAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLIDRYQDGENAGYPTLCKGRYLVDG
ERYHALEEPTSLNTLELLPELMAANIASVKIEGRQRSPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQ
TTLGAYHRKWQ

Sequences:

>Translated_331_residues
MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTEKKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRA
VDMAAQLGADALILADLAMLEYAAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLIDRYQDGENAGYPTLCKGRYLVDG
ERYHALEEPTSLNTLELLPELMAANIASVKIEGRQRSPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQ
TTLGAYHRKWQ
>Mature_331_residues
MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTEKKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRA
VDMAAQLGADALILADLAMLEYAAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLIDRYQDGENAGYPTLCKGRYLVDG
ERYHALEEPTSLNTLELLPELMAANIASVKIEGRQRSPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQ
TTLGAYHRKWQ

Specific function: Unknown

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI1789548, Length=331, Percent_Identity=99.3957703927493, Blast_Score=690, Evalue=0.0,
Organism=Escherichia coli, GI1788397, Length=321, Percent_Identity=28.9719626168224, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081910, Length=293, Percent_Identity=32.7645051194539, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 37005; Mature: 37005

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTEKKLQEAVSFVHQHRRK
CCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHE
LHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEYAAERYPHIERHVSVQASA
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEECC
TNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPLEVFAFGSLCIMSEGRCYLSS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEECCCCHHHHH
YLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLIDRYQDGENAGYPTLCKGRYLVDG
HHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEECC
ERYHALEEPTSLNTLELLPELMAANIASVKIEGRQRSPAYVSQAAKVWRQAIDRCKADPQ
HHHHHCCCCCCCCHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHH
NFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ
HCCCHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTEKKLQEAVSFVHQHRRK
CCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHE
LHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEYAAERYPHIERHVSVQASA
EEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEECC
TNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPLEVFAFGSLCIMSEGRCYLSS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEECCCCHHHHH
YLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLIDRYQDGENAGYPTLCKGRYLVDG
HHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEECC
ERYHALEEPTSLNTLELLPELMAANIASVKIEGRQRSPAYVSQAAKVWRQAIDRCKADPQ
HHHHHCCCCCCCCHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHH
NFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ
HCCCHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]