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The map label for this gene is insD1 [C]

Identifier: 30064483

GI number: 30064483

Start: 3272539

End: 3272961

Strand: Reverse

Name: insD1 [C]

Synonym: S3399

Alternate gene names: 30064483

Gene position: 3272961-3272539 (Counterclockwise)

Preceding gene: 161486430

Following gene: 30064481

Centisome position: 71.16

GC content: 52.96

Gene sequence:

>423_bases
GTGACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCT
TCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGT
TGGGACTTGAACCGAAGAACACGGCGGTGCGTAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAG
CGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAA
CGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATAGGTTAAGTG
ATAACAGATGTCTGGAAATATAG

Upstream 100 bases:

>100_bases
CGATGGTGCTCTGACGGGTTCGAGTTCTGCTGTGATAACGGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATC
GTGAGGCACTGCACTGGGCG

Downstream 100 bases:

>100_bases
GGGCAAATCCACCAGCGTTCTAACGGAATACTGAGATTCAAAGAGAGTGCACGGTCATTATTTTCATCAGTGTAGCGGCT
GTATTGATAATTCAGGCTGT

Product: IS2 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIK
RDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNRLSDNRCLEI

Sequences:

>Translated_140_residues
MTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIK
RDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNRLSDNRCLEI
>Mature_139_residues
TTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKR
DYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNRLSDNRCLEI

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=140, Percent_Identity=98.5714285714286, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1789424, Length=140, Percent_Identity=98.5714285714286, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1789224, Length=140, Percent_Identity=98.5714285714286, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1788305, Length=140, Percent_Identity=98.5714285714286, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1787669, Length=140, Percent_Identity=98.5714285714286, Blast_Score=290, Evalue=2e-80,
Organism=Escherichia coli, GI1786558, Length=140, Percent_Identity=98.5714285714286, Blast_Score=290, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 15869; Mature: 15738

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKN
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECHHHHHHHHHHCCCCCC
TAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCH
REYLRQRACNRLSDNRCLEI
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKN
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECHHHHHHHHHHCCCCCC
TAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCH
REYLRQRACNRLSDNRCLEI
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA