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The map label for this gene is ebgR

Identifier: 30064419

GI number: 30064419

Start: 3200573

End: 3201556

Strand: Direct

Name: ebgR

Synonym: S3322

Alternate gene names: 30064419

Gene position: 3200573-3201556 (Clockwise)

Preceding gene: 161486434

Following gene: 30064420

Centisome position: 69.59

GC content: 51.32

Gene sequence:

>984_bases
ATGGCAACATTAAAAGACATCGCAATCGAAGCTGGCGTATCCCTGGCGACAGTATCCAGGGTCTTAAATGACGATCCGAC
ATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGCGCCCGTA
AACTACAGACAGGCGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAGCAGGAGCTGGAGATCAACGATCCT
TACTATCTGGCGATCCGCCACGGCATTGAAACTCAGTGCGAAAAGCTGGCGATCGAACTGACCAACTGTTATGAACACAA
CGGATTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGCCCGCCCTGCGCGCCGCTGCCAGCG
CGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGC
ATCAGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGG
CAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCG
GCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTT
GCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGTGGCCTGAACATCCCACAAGATATTTCGCTTAT
CAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAGATGATGGGAA
GTCAGGGCGTTAACCTGGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGCTAGTCTTCGTTCCCAGCAAATTA
AAACTGCGCGGCACGACCCGTTAA

Upstream 100 bases:

>100_bases
CAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGTGACCCGATAAGCACTTCCTCTACAATGGGGGCGCACATCAG
GGAAAGTAAAAAAGGTAAAC

Downstream 100 bases:

>100_bases
ATCCCCTTACACACTGTTCGGCAATCATTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGC
ATTTACCTGCTGCTTTTTAG

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR

Sequences:

>Translated_327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR
>Mature_326_residues
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPY
YLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARI
SKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFVA
SDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLK
LRGTTR

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=99.388379204893, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=28.4431137724551, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=27.0588235294118, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=26.4984227129338, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788474, Length=347, Percent_Identity=25.6484149855908, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=26.2536873156342, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=27.0627062706271, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36252; Mature: 36121

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILI
CCCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
GGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC
>Mature Secondary Structure 
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILI
CCCEEEEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEE
GGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]