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The map label for this gene is dnaG

Identifier: 30064414

GI number: 30064414

Start: 3188808

End: 3190553

Strand: Direct

Name: dnaG

Synonym: S3312

Alternate gene names: 30064414

Gene position: 3188808-3190553 (Clockwise)

Preceding gene: 30064413

Following gene: 30064415

Centisome position: 69.33

GC content: 52.29

Gene sequence:

>1746_bases
ATGGCTGGACGAATCCCACGCGTATTCATTAATGATCTGCTGGCACGCACTGACATCGTCGATCTGATCGATGCCCGTGT
GAAGCTGAAAAAGCAGGGCAAGAATTTCCACGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCATTCACCGTTAACG
GTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTC
GAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCA
GATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAAC
CTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTT
GCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGAT
GTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAACGGGTGATGTTCCCCATTCGCGATAAACGCGGTC
GGGTGATTGGTTTTGGCGGGCGCGTGCTGGGCAACGATACCCCCAAATACCTGAACTCGCCGGAAACAGACATTTTCCAT
AAAGGCCGCCAGCTTTACGGTCTTTATGAAGCGCAGCAGGATAACGCTGAACCCAATCGTCTGCTTGTGGTCGAAGGCTA
TATGGACGTGGTGGCGCTGGCGCAATACGGCATTAATTACGCCGTTGCGTCGTTAGGTACGTCAACCACCGCCGATCACA
TACAACTGTTGTTCCGCGCGACCAACAATGTCATTTGCTGTTATGACGGCGACCGTGCAGGCCGCGATGCCGCATGGCGA
GCGCTGGAAACGGCGCTGCCTTACATGACAGACGGCCGTCAGCTACGCTTTATGTTTTTGCCGGATGGCGAAGACCCTGA
CACGCTGGTACGAAAAGAAGGTAAAGAAGCGTTTGAAGCGCGGATGGAGCAGGCGATGCCACTCTCCGCATTTCTGTTTA
ACAGTCTGATGCCGCAAGTTGATCTGAGTACCCCTGACGGGCGCGCACGTTTGAGTACGCTGGCACTGCCATTGATATCG
CAAGTGCCGGGCGAAACGCTGCGAATATATCTTCGTCAGGAATTAGGCAACAAATTAGGCATACTTGATGACAGCCAGCT
TGAACGATTAATGCCAAAAGCGGCAGAGAGCGGCGTTTCTCGCCCTGTTCCGCAGCTAAAACGCACGACCATGCGTATAC
TTATAGGGTTGCTGGTGCAAAATCCAGAATTAGCGACGTTGGTCCCGCCGCTTGAGAATCTGGATGAAAATAAGCTCCCT
GGACTTGGCTTATTCAGAGAACTGGTCAACACTTGTCTCTCCCAGCCAGGTCTGACCACCGGGCAACTTTTAGAGCACTA
TCGTGGTACAAATAATGCTGCCACCCTTGAAAAACTGTCGATGTGGGACGATATAGCAGATAAGAATATTGCTGAGCAAA
CCTTCACCGACTCACTCAACCATATGTTTGATTCGCTGCTTGAACTGCGCCAGGAAGAGTTAATCGCTCGTGAGCGCACG
CATGGTTTAAGCAACGAAGAACGCCTGGAGCTCTGGACATTAAACCAGGAGCTGGCGAAAAAGTGA

Upstream 100 bases:

>100_bases
GCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGA
ATTGCTAAAAATCGGGGCCT

Downstream 100 bases:

>100_bases
TTTAACGGCTTAAGTGCCGAAGAGCGATCGGGAAGCCCCCGACAGCCGCACTGAGAGGCAGCGGCAAATATATAAGTACG
CCCTCGTAATTATCGTTGGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKL
EFVETVEELAAMHNLEVPFEAGSGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
KGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWR
ALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLIS
QVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTTMRILIGLLVQNPELATLVPPLENLDENKLP
GLGLFRELVNTCLSQPGLTTGQLLEHYRGTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERT
HGLSNEERLELWTLNQELAKK

Sequences:

>Translated_581_residues
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKL
EFVETVEELAAMHNLEVPFEAGSGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
KGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWR
ALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLIS
QVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTTMRILIGLLVQNPELATLVPPLENLDENKLP
GLGLFRELVNTCLSQPGLTTGQLLEHYRGTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERT
HGLSNEERLELWTLNQELAKK
>Mature_580_residues
AGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLE
FVETVEELAAMHNLEVPFEAGSGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGFA
PPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFHK
GRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRA
LETALPYMTDGRQLRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLISQ
VPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTTMRILIGLLVQNPELATLVPPLENLDENKLPG
LGLFRELVNTCLSQPGLTTGQLLEHYRGTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTH
GLSNEERLELWTLNQELAKK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=581, Percent_Identity=100, Blast_Score=1202, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_ECO57 (P0ABS6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E91122
- RefSeq:   NP_289641.1
- RefSeq:   NP_311976.1
- ProteinModelPortal:   P0ABS6
- MINT:   MINT-1222290
- EnsemblBacteria:   EBESCT00000026971
- EnsemblBacteria:   EBESCT00000057749
- GeneID:   916216
- GeneID:   958533
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4419
- KEGG:   ecs:ECs3949
- GeneTree:   EBGT00050000010939
- HOGENOM:   HBG573408
- OMA:   GDALPKY
- ProtClustDB:   PRK05667
- BioCyc:   ECOL83334:ECS3949-MONOMER
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:1.10.860.10
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00766
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 65566; Mature: 65434

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEE
CFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAGSGPSQIERHQRQTLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGFAPPGWDNVLKRFGGNPENRQ
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
SLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
HHHHCCCEEECCCCCCHHHHHHHCCCCEECCCCCEEEECCEECCCCCHHHCCCCCHHHHH
KGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRA
CCCHHEEHHHHHCCCCCCCEEEEEECHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHH
TNNVICCYDGDRAGRDAAWRALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEGKEAFEA
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHH
RMEQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLISQVPGETLRIYLRQELGNKLG
HHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
ILDDSQLERLMPKAAESGVSRPVPQLKRTTMRILIGLLVQNPELATLVPPLENLDENKLP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCCCC
GLGLFRELVNTCLSQPGLTTGQLLEHYRGTNNAATLEKLSMWDDIADKNIAEQTFTDSLN
CHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HMFDSLLELRQEELIARERTHGLSNEERLELWTLNQELAKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCC
>Mature Secondary Structure 
AGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEE
CFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAGSGPSQIERHQRQTLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGFAPPGWDNVLKRFGGNPENRQ
HHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
SLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
HHHHCCCEEECCCCCCHHHHHHHCCCCEECCCCCEEEECCEECCCCCHHHCCCCCHHHHH
KGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHIQLLFRA
CCCHHEEHHHHHCCCCCCCEEEEEECHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHH
TNNVICCYDGDRAGRDAAWRALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEGKEAFEA
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHH
RMEQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLISQVPGETLRIYLRQELGNKLG
HHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
ILDDSQLERLMPKAAESGVSRPVPQLKRTTMRILIGLLVQNPELATLVPPLENLDENKLP
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCCCC
GLGLFRELVNTCLSQPGLTTGQLLEHYRGTNNAATLEKLSMWDDIADKNIAEQTFTDSLN
CHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HMFDSLLELRQEELIARERTHGLSNEERLELWTLNQELAKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796