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The map label for this gene is mycA [H]

Identifier: 30064329

GI number: 30064329

Start: 3107379

End: 3109067

Strand: Direct

Name: mycA [H]

Synonym: S3222

Alternate gene names: 30064329

Gene position: 3107379-3109067 (Clockwise)

Preceding gene: 30064328

Following gene: 30064330

Centisome position: 67.56

GC content: 52.93

Gene sequence:

>1689_bases
ATGCGCTATGCCGATTTTCCAACGCTGGTTGATGCTTTGGACTACGCCGCTCTGAGTAGAGCCGGAATGAATTTTTATGA
CAGACGTTGCCAACTTGAAGATCAACTGGAATATCAGACATTAAAAACGCGTGCCGAAGCTGGTGCGAAGCGGTTGTTAT
CGCTGAACCTGAAAAAAGGCGAACGCGTGGCGCTGATTGCCGAAACGAGTAGCGGGTTTGTAGAGGCTTTTTTTGCCTGT
CAGTATGCTGGCTTAGTCGCCGTCCCGTTGGCGATTCCAATGGGCGTCGGTCAGCGGGATTCCTGGAGCGCCAAACTCCA
GGGTTTACTGGCAAGTTGCCAGCCCGCAGCCATTATCACTGGTGATGAGTGGTTGCCACTGGTCAATGCCGCGACGCATA
ACAACCCCGAATTACATGTTTTAAGCCACGCTTGGTTTAAGGCATTACCGGAAGCCGATGTTGCGCTCCAGCGTCCAGTT
CCGAACGATATCGCCTACCTCCAGTACACCTCCGGCAGCACCCGTTTTCCCCGTGGCGTCATTATCACCCATCGCGAAGT
GATGGCTAATCTACGTGCTATAAGCCACGATGGCATTAAATTACGCCCTAGCGACCGCTGCGTCTCCTGGCAGCCTTTCT
ACCATGATATGGGACTGGTCGGCTTTCTCCTGCCCCCCGTCGCCACGCAACTTTCAGTAGATTATTTGCGCACTCAGGAT
TTTGCCATGCGTCCTTTGCAATGGCTTAAATTGATCAGTAAAAATCGCGGCACCGTTTCCGTTGCGCCGCCGTTTGGCTA
TGAATTGTGCCAACGCCGCGTGAATGAAAAAGATCTCGCTGAACTGGATCTTTCCTGCTGGCGCGTCGCTGGTATTGGTG
CTGAGCCGATCTCCGCAGAACAACTCCATCAATTCGCTGAATGTTTCCGTCAGGTTAACTTTGACAATAAAACTTTCATG
CCGTGCTACGGACTGGCAGAAAATGCGCTGGCTGTCAGCTTCTCTGATGAAGCCTCCGGGGTTGTGGTTAACGAAGTGGA
TCGCGACATCCTCGAATATCAGGGTAAAGCCGTCGCGCCGGGTGCAGAGACACGCGCCGTATCGACTTTCGTCAACTGCG
GCAAAGCGTTGCCGGAACATGGTATTGAAATCCGCAATGAAGCAGGTATGCCGGTCGCGGAACGTGTGGTAGGCCATATT
TGCATCTCCGGTCCCAGTCTGATGAGCGGTTACTTTGGCGACCAGGTTTCGCAAGACGAGATTGCCGCGACGGGCTGGTT
AGACACCGGCGACCTCGGTTATCTGCTGGACGGTTATCTGTATGTCACCGGACGCATTAAAGATCTGATTATTATTCGTG
GCCGTAATATCTGGCCGCAGGATATTGAATATATAGCGGAACAGGAACCGGAAATTCATTCTGGCGATGCGATTGCTTTT
GTTACCGCCCAGGAAAAAATCATTTTGCAGATCCAGTGTCGGATCAGCGACGAAGAACGTCGCGGGCAGCTTATCCACGC
GCTGGCGGCACGGATCCAAAGCGAATTTGGCGTGACCGCGGCTATCGATCTGTTGCCGCCCCACAGTATTCCCCGAACGT
CCTCCGGCAAGCCTGCCCGTGCGGAAGCGAAAAAACGTTATCAGAAGGCTTATGCTGCCAGTCTTAATGTGCAGGAATCC
CTGGCATAA

Upstream 100 bases:

>100_bases
TTATGCCTATATAATCCACCGCATTATTGTCATTTTATTTTTGATATCCAATTTTTTCGTGGTGTATATGTCTAATAAAA
TCTTTACGCATTCCCTACCT

Downstream 100 bases:

>100_bases
ATCAAACTGTCGCGGTGACGGGCGCTACCGGGTTTATCGGTAAATATATTATCGATAACCTGCTCGCCCGCGGCTTTCAC
GTTCGCGCATTGACGCGTAC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFAC
QYAGLVAVPLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQD
FAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPVAERVVGHI
CISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAF
VTAQEKIILQIQCRISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQES
LA

Sequences:

>Translated_562_residues
MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFAC
QYAGLVAVPLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQD
FAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPVAERVVGHI
CISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAF
VTAQEKIILQIQCRISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQES
LA
>Mature_562_residues
MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFAC
QYAGLVAVPLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQD
FAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPVAERVVGHI
CISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAF
VTAQEKIILQIQCRISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQES
LA

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=563, Percent_Identity=23.0905861456483, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI187761345, Length=499, Percent_Identity=23.8476953907816, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI187761343, Length=499, Percent_Identity=23.8476953907816, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI55749758, Length=544, Percent_Identity=21.1397058823529, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1788107, Length=584, Percent_Identity=23.8013698630137, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI145693145, Length=478, Percent_Identity=25.3138075313808, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=551, Percent_Identity=22.1415607985481, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=558, Percent_Identity=22.9390681003584, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=509, Percent_Identity=22.3968565815324, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18859661, Length=399, Percent_Identity=23.8095238095238, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24654656, Length=619, Percent_Identity=22.6171243941842, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24581924, Length=388, Percent_Identity=24.2268041237113, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62038; Mature: 62038

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTRAEAGAKRLLSLNLKKG
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCC
ERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSWSAKLQGLLASCQPAAIIT
CEEEEEEECCCHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPVPNDIAYLQYTSGSTRFPRGV
CCCCCEEEECCCCCCCCEEEEHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCCCCCCCE
IITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQD
EEECHHHHHHHHHHCCCCEEECCCCCCEECCCHHHHCCHHHHHHCCHHHHHHHHHHHCCH
FAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAE
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCHHHHHHHCHHHHEEECCCCCCCCHH
QLHQFAECFRQVNFDNKTFMPCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAP
HHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHCCCEECC
GAETRAVSTFVNCGKALPEHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDE
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHEECCCHHHHCCCCCCCCCCH
IAATGWLDTGDLGYLLDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAF
HEEECCCCCCCCHHEECCEEEEEECEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEE
VTAQEKIILQIQCRISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPAR
EEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCH
AEAKKRYQKAYAASLNVQESLA
HHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTRAEAGAKRLLSLNLKKG
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCC
ERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSWSAKLQGLLASCQPAAIIT
CEEEEEEECCCHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPVPNDIAYLQYTSGSTRFPRGV
CCCCCEEEECCCCCCCCEEEEHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCCCCCCCE
IITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQD
EEECHHHHHHHHHHCCCCEEECCCCCCEECCCHHHHCCHHHHHHCCHHHHHHHHHHHCCH
FAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAE
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCHHHHHHHCHHHHEEECCCCCCCCHH
QLHQFAECFRQVNFDNKTFMPCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAP
HHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHCCCEECC
GAETRAVSTFVNCGKALPEHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDE
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHEECCCHHHHCCCCCCCCCCH
IAATGWLDTGDLGYLLDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAF
HEEECCCCCCCCHHEECCEEEEEECEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEE
VTAQEKIILQIQCRISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPAR
EEECCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCH
AEAKKRYQKAYAASLNVQESLA
HHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]