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The map label for this gene is yghK

Identifier: 30064325

GI number: 30064325

Start: 3096365

End: 3097951

Strand: Reverse

Name: yghK

Synonym: S3215

Alternate gene names: 30064325

Gene position: 3097951-3096365 (Counterclockwise)

Preceding gene: 30064326

Following gene: 30064324

Centisome position: 67.36

GC content: 52.55

Gene sequence:

>1587_bases
ATGGTTACCTGGACCCAAATGTATATGCCGATGGGAGGACTGGGGCTATCCGCTCTGGTCGCCCTGATCCCGATAATATT
CTTCTTCGTTGCACTCGCGGTATTACGTCTGAAAGGACATGTTGCTGGAGCAATAACCCTTATATTATCTATCCTGATTG
CGATATTCGCCTTTAAAATGCCGATTGATATGGCATTTGCTGCTGCGGGCTATGGCTTTATTTATGGATTATGGCCAATT
GCGTGGATTATTGTCGCGGCGGTGTTCCTGTATAAATTAACCGTTGCCAGCGGGCAGTTCGATATTATCCGCAGCTCGGT
TATCTCCATCACCGACGATCAGCGTTTGCAGGTGTTACTGATTGGTTTCTCCTTTGGTGCGTTGCTGGAAGGAGCTGCTG
GCTTTGGTGCGACGGTGGCGTTTGGTGCGTTGGGCGTGCCGATTCTGGTCGCCGGTCAGGTAACGGGAATCGATCCGTTC
CACATTGGCGCAATGGCGGGACGTCAGTTACCGTTCCTGTCGGTTCTTGTGCCGTTCTGGCTGGTAGCAATGATGGACGG
CTGGAAAGGGGTGAAAGAGACGTGGCCAGCGGCGCTGGTTGCTGGGGGAAGCTTCGCTGTCACTCAGTTCTTTACCTCTA
ACTATATTGGTCCGGAACTGCCGGATATTACTTCGGCGCTGGTGAGTATCGTCTCACTCGCTTTATTCCTTAAAGTCTGG
CGGCCGAAAAATACCGAAACGGCAATCAGCATGGGGCAATCCGCAGGTGCGATGGTGGTAAATAAGCCATCTTCTGGCGG
TCCCGTGCCTTCAGAATATAGTCTGGGGCAAATCATTCGTGCGTGGTCACCGTTTTTAATCTTAACGGTGCTGGTCACCA
TCTGGACCATGAAGCCGTTTAAAGCGTTATTTGCTCCGGGCGGCGCGTTTTATTCACTGGTGATTAATTTCCAGATCCCT
CATTTGCATCAACAAGTGTTGAAAGCGGCACCCATTGTCGCCCAACCAACGCCAATGGATGCGGTGTTTAAATTCGACCC
TCTCTCGGCTGGCGGCACCGCTATTTTTATTGCGGCGATTATCTCTATCTTCATCCTCGGCGTGGGCATCAAGAAAGGGA
TTGGCGTCTTTGCCGAAATGCTAATTAGTCTGAAGTGGCCGATATTGTCGATTGGCATGGTGCTGGCATTCGCCTTCGTC
ACCAACTATTCCGGCATGTCCACCACGCTGGCGCTGGTACTGGCAGGTACAGGCGTGATGTTCCCGTTTTTCTCACCGTT
TCTCGGCTGGCTGGGCGTATTCCTTACCGGGTCGGACACTTCTTCAAACGCCCTGTTTGGTTCACTGCAATCGACCACGG
CGCAGCAAATCAACGTCTCTGACACTCTGCTGGTGGCAGCAAATACCAGCGGCGGCGTAACTGGCAAGATGATCTCCCCG
CAATCTATCGCCGTGGCCTGCGCCGCGACGGGCATGGTGGGCCGAGAATCTGAACTGTTCCGCTACACCGTGAAGCACAG
TCTGATTTTTGCCAGCGTTATCACCCTGCTGCAGGCGTATGTGTTTACCGGGATGTTAGTCTCGTAA

Upstream 100 bases:

>100_bases
GCTGTAAAGTATAACGCCTATATTTTACAGTTCAGTCCTCTTTTGCTTATTACAACCTTAAGTATATCAAGCATATAAAG
ATAATAAGAGACTGAACAAT

Downstream 100 bases:

>100_bases
ATCAATAAAGCCGGATGAAGGTAAATATCACCCGGCTTTCCATTTGTCTGTCGTTTATCTCGCCAGGGAACCGTTTTTAC
GATTGGCTTCTTTATACGTA

Product: putative permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPI
AWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVW
RPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIP
HLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIGMVLAFAFV
TNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMISP
QSIAVACAATGMVGRESELFRYTVKHSLIFASVITLLQAYVFTGMLVS

Sequences:

>Translated_528_residues
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPI
AWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVW
RPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIP
HLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIGMVLAFAFV
TNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMISP
QSIAVACAATGMVGRESELFRYTVKHSLIFASVITLLQAYVFTGMLVS
>Mature_528_residues
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPI
AWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVW
RPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIP
HLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIGMVLAFAFV
TNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMISP
QSIAVACAATGMVGRESELFRYTVKHSLIFASVITLLQAYVFTGMLVS

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=560, Percent_Identity=93.9285714285714, Blast_Score=982, Evalue=0.0,
Organism=Escherichia coli, GI1790031, Length=558, Percent_Identity=57.8853046594982, Blast_Score=595, Evalue=1e-171,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 55861; Mature: 55861

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKM
CCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEE
IGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFW
EEECHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHH
LVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVW
HHHHHHCCCCHHHHCCHHEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
RPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPF
CCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
KALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAI
HHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
ISIFILGVGIKKGIGVFAEMLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVM
HHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
FPFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMISP
HHHHHHHHHHHHEEEECCCCCCCHHEECHHHCHHHEECCCCEEEEEECCCCCCEECEECC
QSIAVACAATGMVGRESELFRYTVKHSLIFASVITLLQAYVFTGMLVS
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKM
CCEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEE
IGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFW
EEECHHHHHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHH
LVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVW
HHHHHHCCCCHHHHCCHHEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
RPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPF
CCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
KALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAI
HHHHCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHH
ISIFILGVGIKKGIGVFAEMLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVM
HHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
FPFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMISP
HHHHHHHHHHHHEEEECCCCCCCHHEECHHHCHHHEECCCCEEEEEECCCCCCEECEECC
QSIAVACAATGMVGRESELFRYTVKHSLIFASVITLLQAYVFTGMLVS
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]