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The map label for this gene is intB [H]

Identifier: 30064288

GI number: 30064288

Start: 3044913

End: 3046175

Strand: Direct

Name: intB [H]

Synonym: S4822

Alternate gene names: 30064288

Gene position: 3044913-3046175 (Clockwise)

Preceding gene: 30064287

Following gene: 30064289

Centisome position: 66.2

GC content: 48.77

Gene sequence:

>1263_bases
ATGGCACTGACTGACGCAAAAATCCGGGCTGCAAAGCCCACTGACAAGGCTTATAAACTCACTGACGGGGCCGGCATGTT
CCTGCTGGTTCATCCTAATGGTTCCCGTTACTGGCGTCTCCGTTATCGTATTCTGGGTAAGGAGAAGACTCTGGCACTTG
GTGTGTATCCAGAAGTTTCTCTCTCCGAAGCTCGTACAAAACGGGATGAGGCCCGAAAACTGATTTCAGAGGGGATTGAC
CCTTGCGAACAGAAAAGAGTTAAAAAAGTTGTCCCTGATTTACAGCTCTCTTTTGAACATATTGCACGACGCTGGCATGC
CAGTAATAAACAATGGGCACAATCACACAGCGATAAAGTACTCAAAAGCCTCGAGACACACGTTTTCCCCTTTATCGGCA
ACCGGGATATCACAACACTCAATACCCCGGACCTGCTTATCCCTGTTCGTGCTGCAGAAGCAAAACAAATTTATGAAATC
GCCAGTCGTCTGCAGCAAAGAATATCTGCTGTAATGCGTTACGCCGTACAGTCTGGCATCATCAGATATAACCCGGCTCT
GGATATGGCTGGTGCATTGACCACGGTAAAACGCCAGCATCGCCCTGCTCTGGAGCTTTCACGCCTGCCTGAACTTCTGT
CGCGTATTGACGGTTATAAAGGCCAGCCTGTCACCCGACTGGCAGTTATGCTGAATTTACTGGTTTTTATTCGTTCCAGT
GAACTCAGATATGCCCGCTGGTCGGAAATTGATATTGACAATTCCATGTGGACTATTCCAGCCGAACGCGAACCTCTGCC
AGGCGTAAAATTCTCACACCGGGGATCCAAGATGCGAACACCACATCTTGTGCCACTCAGCAAACAGGTTGTAGCCATAC
TGGCAGAACTTCAGACATGGGCTGGTGAAAATGGTCTGATCTTTACGGGTGCACATGACCCGCGTAAACCAATCAGTGAA
AATACTGTAAATAAAGCCCTGAGGGTGATGGGGTATGACACAACCCAGGAAGTCTGTGGCCATGGATTCCGGGCGATGGC
GTGCAGTGCATTGATTGAATCAGGTTTGTGGTCCCGCGATGCTGTGGAACGTCAGATGAGCCATCAGGAGCGTAATGGTG
TACGTGCTGCGTATATCCATAAAGCAGAACATCTGGAAGAACGGCGACTGATGTTGCAATGGTGGGCCGATTTTCTAGAT
GCGAACCGGGAGAAGGGTATCAGCCCGTTTGAATATGCAAAGATTAACAATCCATTAAAATAG

Upstream 100 bases:

>100_bases
AGATTCCCCCCACATCAACCCCATTGTGTGGGTATAATTGCGGGTATACCCCAGTTCGACAGAATTTGTACCCTCTTTAA
CGCATTCAGAAGGCTGAACT

Downstream 100 bases:

>100_bases
TAAGCAGTCCGGGCAATCAGCCCGGACTGCTTTAACTAATTATTTTTCTTTGTAAATGATGACGAAGATTGATGTTTCTG
ACAAGTTACTCCGGCTTCCA

Product: putative P4-type integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPEVSLSEARTKRDEARKLISEGID
PCEQKRVKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSS
ELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISE
NTVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLD
ANREKGISPFEYAKINNPLK

Sequences:

>Translated_420_residues
MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPEVSLSEARTKRDEARKLISEGID
PCEQKRVKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSS
ELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISE
NTVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLD
ANREKGISPFEYAKINNPLK
>Mature_419_residues
ALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDP
CEQKRVKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIA
SRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSSE
LRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISEN
TVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDA
NREKGISPFEYAKINNPLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI145693166, Length=414, Percent_Identity=35.7487922705314, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1788974, Length=418, Percent_Identity=35.1674641148325, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1788690, Length=404, Percent_Identity=35.1485148514851, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47859; Mature: 47728

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPEVS
CCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEECCCCEEEEECCCCCC
LSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRYAVQSGI
HHHHHHHHCCEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSS
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
ELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTW
CCCEECCCCEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRD
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCHH
AVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREKGISPFEYAKINNPLK
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
ALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPEVS
CCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCEEEEEEEEECCCCEEEEECCCCCC
LSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRYAVQSGI
HHHHHHHHCCEECCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSS
EEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
ELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAELQTW
CCCEECCCCEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRD
CCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHCCCCCHH
AVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREKGISPFEYAKINNPLK
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]