Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 30064234

GI number: 30064234

Start: 2997641

End: 2998804

Strand: Reverse

Name: pgk

Synonym: S3111

Alternate gene names: 30064234

Gene position: 2998804-2997641 (Counterclockwise)

Preceding gene: 30064235

Following gene: 30064233

Centisome position: 65.2

GC content: 52.32

Gene sequence:

>1164_bases
ATGTCTGTAATTAAGATGACCGATCTGGATCTTGCTGGGAAACGTGTATTTATCCGTGCGGATCTGAACGTACCAGTAAA
AGACGGGAAAGTAACCAGCGACGCGCGTATCCGTGCTTCTCTGCCGACCATCGAACTGGCCCTGAAACAAGGCGCAAAAG
TGATGGTAACTTCCCACCTGGGTCGTCCTACCGAAGGCGAGTACAACGAAGAATTCTCTCTGCTGCCGGTTGTTAACTAC
CTGAAAGACAAACTGTCTAACCCGGTTCGTCTGGTTAAAGATTACCTCGACGGCGTTGACGTTGCTGAAGGTGAACTGGT
TGTTCTGGAAAACGTTCGCTTCAACAAAGGCGAGAAGAAAGACGACGAAACCCTGTCCAAAAAATACGCTGCACTGTGTG
ACGTGTTCGTAATGGACGCATTCGGTACTGCTCACCGCGCGCAGGCTTCTACTCACGGTATCGGTAAATTCGCTGACGTT
GCGTGCGCAGGCCCGCTGCTGGCAGCTGAACTGGACGCGCTGGGTAAAGCACTGAAAGAACCTGCTCGCCCGATGGTGGC
TATCGTTGGTGGTTCTAAAGTATCTACCAAACTGACCGTTCTGGACTCCCTGTCTAAAATCGCTGACCAGCTGATTGTTG
GTGGTGGTATCGCTAACACCTTTATCGCGGCACAAGGCCACGATGTGGGTAAATCCCTGTACGAAGCTGACCTGGTTGAC
GAAGCTAAACGTCTGCTGACCACCTGCAACATCCCGGTTCCGTCTGATGTTCGCGTAGCAACCGAGTTCTCTGAAACTGC
ACCGGCTACCCTGAAATCTGTTAACGATGTGAAAGCTGACGAGCAGATCCTGGATATCGGTGATGCTTCCGCTCAGGAAC
TGGCTGAAATCCTGAAGAATGCGAAAACCATTCTGTGGAACGGTCCGGTTGGCGTGTTCGAATTCCCGAACTTCCGCAAA
GGTACTGAAATCGTGGCTAACGCTATCGCAGACAGCGAAGCGTTCTCCATCGCTGGCGGCGGCGACACTCTGGCAGCAAT
CGACCTGTTCGGCATTGCTGACAAAATCTCCTACATCTCCACTGGCGGCGGCGCATTCCTCGAATTCGTGGAAGGTAAAG
TACTGCCTGCAGTAGCGATGCTCGAAGAGCGCGCTAAGAAGTAA

Upstream 100 bases:

>100_bases
AACCGAATGCTCGACACGACGTTAGCTATGGCTACTGTTGCTTTCAGGTAAGACGCAAGCAGCGTCTGCAAAACTTTTAG
AATCAACGAGAGGATTCACC

Downstream 100 bases:

>100_bases
AAAATCACAGGGCAGGGAAACCTGCACTTGTTTCAGCGCGCTTTTAGAGCACGCATTTTTTCATACTCTAAATAATTCGA
GTTGCAGGAAGGCGACAAGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNY
LKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
EAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRK
GTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK

Sequences:

>Translated_387_residues
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNY
LKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
EAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRK
GTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK
>Mature_386_residues
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYL
KDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVA
CAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVDE
AKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKG
TEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=36.4963503649635, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=36.2530413625304, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1789294, Length=387, Percent_Identity=100, Blast_Score=776, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17508823, Length=413, Percent_Identity=39.4673123486683, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319857, Length=416, Percent_Identity=39.6634615384615, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24581203, Length=416, Percent_Identity=39.9038461538462, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=39.0776699029126, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ECO24 (A7ZR34)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001464263.1
- ProteinModelPortal:   A7ZR34
- SMR:   A7ZR34
- STRING:   A7ZR34
- EnsemblBacteria:   EBESCT00000021335
- GeneID:   5585986
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_3254
- eggNOG:   COG0126
- GeneTree:   EBGT00050000011026
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   ECOL331111:ECE24377A_3254-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41119; Mature: 40987

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 113-113 BINDING 146-146 BINDING 197-197 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHL
CCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVACAGPLLAAELDALGKALKE
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCHHHHHHHHHHHHH
EAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKN
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYIS
CCEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHEE
TGGGAFLEFVEGKVLPAVAMLEERAKK
CCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTIELALKQGAKVMVTSHL
CEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVAEGELVVLENVRFNKGEKK
CCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADVACAGPLLAAELDALGKALKE
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFIAAQGHDVGKSLYEADLVD
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCHHHHHHHHHHHHH
EAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVNDVKADEQILDIGDASAQELAEILKN
HHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFPNFRKGTEIVANAIADSEAFSIAGGGDTLAAIDLFGIADKISYIS
CCEEEECCCCCCEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHEE
TGGGAFLEFVEGKVLPAVAMLEERAKK
CCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA