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The map label for this gene is argA

Identifier: 30064160

GI number: 30064160

Start: 2904258

End: 2905589

Strand: Direct

Name: argA

Synonym: S3026

Alternate gene names: 30064160

Gene position: 2904258-2905589 (Clockwise)

Preceding gene: 30064156

Following gene: 30064172

Centisome position: 63.14

GC content: 52.7

Gene sequence:

>1332_bases
GTGGTAAAGGAACGTAAAACCGAGCTGGTCGAGGGATTCCGCCATTCGGTTCCCTATATCAATACCCACCGGGGAAAAAC
GTTTGTCATCATGCTCGGCGGTGAAGCCATTGAGCATGAGAATTTCTCCAGTATCGTTAATGATATCGGGTTGTTGCACA
GCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCG
CTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGA
TATTACTGCTCGCCTGTCAATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTTAGTGGCAATTTTA
TTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCG
CTCCATCGTCAACTGGAAAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAATCT
GACCTCGGAAGAGATTGCCACTCAACTAGCCATCAAACTGAAAGCTGAAAAGATGATTGGTTTTTGCTCTTCCCAGGGCG
TTACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCACGGGTAGAAGCCCAGGAAGAGAAA
GGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGCCGCTGTCATTT
AATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAA
GCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCGCTGGAGCAGCAAGGT
ATTCTGGTGCGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTAT
TGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCA
GTTCATCACGGGGCGAGGTTCTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGCGGCTTAAGCAAATTGTTTGTGCTG
ACCACGCGCAGTATTCACTGGTTCCAGGAACGTGGATTTACCCCGGTGGATATTGATTTACTGCCCGAGAGCAAAAAGCA
GTTGTACAACTACCAGCGTAAATCCAAAGTGTTGATGGCGGATTTAGGGTAA

Upstream 100 bases:

>100_bases
TGATAAAAAAGTCGCTCTCGCATAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACAATAATTTCAAATA
ATCATGCAAAGGGGTGTGCC

Downstream 100 bases:

>100_bases
GCGATGAAAATCACCGGATGCGGCAGGCGTAACACTCGTGCGTCGCATCCGGCAATTACGTTTATTCCCGCGAACTAAAC
AACGCCGCCAGACCACTGCG

Product: N-acetylglutamate synthase

Products: NA

Alternate protein names: N-acetylglutamate synthase; AGS; NAGS [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG

Sequences:

>Translated_443_residues
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG
>Mature_443_residues
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1789181, Length=443, Percent_Identity=99.548532731377, Blast_Score=909, Evalue=0.0,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR001048
- InterPro:   IPR010167 [H]

Pfam domain/function: PF00696 AA_kinase; PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.1 [H]

Molecular weight: Translated: 49210; Mature: 49210

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLV
CCCHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
VVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTP
EEECCCCCCCCCHHHCCCCCCEECCEEEECHHHHHHHHHHCCEEEEEEEEEEEEEECCCC
LQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDALHRQLESGAIVLMGPVAVSV
CCCCEEEEEECCEEEEECCCCCCCCHHHCCCCCEECCHHHHHHHHHCCCEEEEECEEEEE
TGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
ECCCCCCCHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHC
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRR
CCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKI
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCEEEEEEECCCEEEEEEECCCCHHHH
GEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVLTTRSIHWFQERGFTPVDIDL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCEEEEEE
LPESKKQLYNYQRKSKVLMADLG
CCCHHHHHHHHHHHCEEEEECCC
>Mature Secondary Structure
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLV
CCCHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
VVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTP
EEECCCCCCCCCHHHCCCCCCEECCEEEECHHHHHHHHHHCCEEEEEEEEEEEEEECCCC
LQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDALHRQLESGAIVLMGPVAVSV
CCCCEEEEEECCEEEEECCCCCCCCHHHCCCCCEECCHHHHHHHHHCCCEEEEECEEEEE
TGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
ECCCCCCCHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHC
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRR
CCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKI
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCEEEEEEECCCEEEEEEECCCCHHHH
GEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVLTTRSIHWFQERGFTPVDIDL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCEEEEEE
LPESKKQLYNYQRKSKVLMADLG
CCCHHHHHHHHHHHCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]