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The map label for this gene is cysJ

Identifier: 30064113

GI number: 30064113

Start: 2848826

End: 2850625

Strand: Reverse

Name: cysJ

Synonym: S2973

Alternate gene names: 30064113

Gene position: 2850625-2848826 (Counterclockwise)

Preceding gene: 30064117

Following gene: 30064112

Centisome position: 61.98

GC content: 56.11

Gene sequence:

>1800_bases
ATGACGACACAGGTCCCACCTTCCGCGTTGCTTCCGTTGAACCCGGAGCAACTGGCACGCCTTCAGGCGGCCACGACCGA
TTTAACGCCTACCCAGCTTGCCTGGGTTTCTGGCTATTTCTGGGGCGTGCTCAACCAGCAGCCTGTTGCGCTTGCAGTGA
CGCCAGCGCCCGCCGCAGAAATGCCGGGTATAACGATTATCTCTGCTTCGCAAACCGGCAATGCGCGCCGGGTTGCTGAA
GCATTACGCGATGATTTATTAGCAGCAAAACTGAACGTTAAGCTGGTGAACGCGGGCGACTATAAATTCAAACAAATCGC
CAGCGAAAAACTGCTCATCGTAGTGACGTCAACGCAAGGGGAAGGGGAACCGCCGGAAGAAGCCGTCGCGCTGCATAAGT
TCCTGTTCTCCAAAAAAGCGCCAAAGCTGGAAAACACCGCGTTTGCCGTGTTTAGCCTCGGCGATAGCTCTTATGAATTT
TTCTGCCAGTCCGGGAAAGATTTTGACAGCAAGCTGGCGGAACTGGGGGGTGAACGCCTGCTCGACCGTGTCGATTCTGA
TGTTGAATACCAGGCTGCTGCCAGCGAGTGGCGCGCCCGCGTGGTTGATGCGCTTAAATCGCGTGCGCCTGTCGCGGCAC
CTTCGCAATCCGTCGCTACTGGCGCGGTAAATGAAATCCACACCAGCCCGTACAGCAAAGACGCGCCGCTGGTGGCTAGC
CTCTCTGTTAACCAGAAAATTACTGGGCGTAACTCTGAAAAAGACGTTCGCCATATCGAAATTGACTTAGGCGACTCGGG
CCTGCGTTACCAGCCGGGTGACGCGCTGGGCGTCTGGTATCAGAACGATCCGGCACTGGTGAAAGAACTTGTCGAACTGC
TGTGGCTGACAGGCGATGAACCTGTCACTGTCGAGGGCAAAACGTTGCCTCTGAACGAAGCGCTACAGTGGCACTTCGAA
CTGACCGTCAACACCGCCAACATTGTTGAGAATTACGCCACGCTTACCCGCAGTGAAACACTGCTGCCGCTGGTGGGCGA
TAAAGCGAAGTTACAGCATTACGCCGCGACGACGCTGATTGTTGACATGGTGCGTTTCTCCCCGGCACAGCTTGATGCCG
AAGCGCTAATTAATCTGCTGCGCCCGCTGACCCCGCGTCTCTATTCCATTGCCTCCTCGCAGGCGGAAGTCGAGAACGAA
GTACACGTCACCGTTGGTGTGGTGCGTTACGACGTGGAAGGCCGCGCCCGTGCCGGTGGTGCCTCCAGCTTCCTCGCTGA
CCGCGTGGAAGAAGAGGGCGAAGTCCGCGTATTTATCGAACATAACGATAACTTCCGCCTGCCCGCTAACCTGGAAACCC
CGGTGATTATGATTGGCCCAGGCACCGGTATTGCGCCGTTCCGCGCCTTTATGCAGCAACGCGCCGCTGACGAAGCGCCA
GGTAAAAACTGGCTGTTCTTTGGCAATCCGCATTTTACGGAAGACTTCCTGTATCAGGTGGAGTGGCAGCGCTACGTCAA
AGAGGGTGTGCTGACGCGGATCGATCTCGCCTGGTCGCGCGACCAAAAAGAAAAAGTTTACGTACAAGATAAACTGCGCG
AACAGGGCGCGGAGCTGTGGCGCTGGATCAATGACGGTGCCCACATTTATGTCTGCGGCGACGCTAATCGCATGGCGAAA
GACGTTGAGCAGGCACTTCTGGAAGTGATTGCCGAATTTGGTGGCATGGACACCGAAGCGGCGGATGAATTTTTAAGTGA
GCTGCGCGTAGAGCGCCGTTATCAGCGAGATGTCTACTAA

Upstream 100 bases:

>100_bases
TTAATCCACACCGTTCACCCCGTTAACCTTACCTTCTCTTCTGTTTTATGGGCGCTGACAGGGCGCAGAAACAGCTTTGC
TTACTGGAACATAACGACGC

Downstream 100 bases:

>100_bases
TGAGCGAAAAACATCCAGGGCCTTTAGTGGTCGAAGGAAAACTGACAGACGCCGAGCGCATGAAGCTTGAAAGCAACTAC
CTGCGCGGCACCATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPVALAVTPAPAAEMPGITIISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENE
VHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Sequences:

>Translated_599_residues
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPVALAVTPAPAAEMPGITIISASQTGNARRVAE
ALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFE
LTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENE
VHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQRAADEAP
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAK
DVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
>Mature_598_residues
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEA
LRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFF
CQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVASL
SVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFEL
TVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEV
HVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQRAADEAPG
KNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKD
VEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=601, Percent_Identity=30.4492512479201, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI7657393, Length=595, Percent_Identity=30.2521008403361, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI221316709, Length=595, Percent_Identity=30.2521008403361, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI24041029, Length=597, Percent_Identity=30.6532663316583, Blast_Score=222, Evalue=9e-58,
Organism=Homo sapiens, GI221316705, Length=604, Percent_Identity=29.8013245033113, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI10835173, Length=651, Percent_Identity=29.3394777265745, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI221316707, Length=525, Percent_Identity=30.4761904761905, Blast_Score=199, Evalue=8e-51,
Organism=Homo sapiens, GI40254422, Length=649, Percent_Identity=27.2727272727273, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI169790956, Length=437, Percent_Identity=28.3752860411899, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI169790958, Length=437, Percent_Identity=28.3752860411899, Blast_Score=156, Evalue=6e-38,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=98.6644407345576, Blast_Score=1210, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=636, Percent_Identity=29.559748427673, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17566446, Length=583, Percent_Identity=26.4150943396226, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531441, Length=394, Percent_Identity=25.3807106598985, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321832, Length=636, Percent_Identity=28.4591194968553, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321143, Length=397, Percent_Identity=31.2342569269521, Blast_Score=160, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6325305, Length=626, Percent_Identity=23.3226837060703, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI17137192, Length=624, Percent_Identity=27.724358974359, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24582192, Length=510, Percent_Identity=29.4117647058824, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24583543, Length=523, Percent_Identity=32.5047801147228, Blast_Score=187, Evalue=3e-47,
Organism=Drosophila melanogaster, GI78706876, Length=523, Percent_Identity=32.5047801147228, Blast_Score=187, Evalue=3e-47,
Organism=Drosophila melanogaster, GI78706872, Length=523, Percent_Identity=32.5047801147228, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24660907, Length=595, Percent_Identity=26.218487394958, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24660903, Length=595, Percent_Identity=26.218487394958, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24647438, Length=437, Percent_Identity=21.7391304347826, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 66344; Mature: 66212

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPVALAVTPAPAAE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
MPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLTGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTLI
CEEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDVEGRARAGG
HHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVLNQQPVALAVTPAPAAE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
MPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYKFKQIASEKLLIVVTSTQG
CCCEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHCCCCEEEEEECCCC
EGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEFFCQSGKDFDSKLAELGGERL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHCCCCCCHHHHHHHHCHHHH
LDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSVATGAVNEIHTSPYSKDAPLVAS
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCCCCCCCEEEE
LSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQNDPALVKELVELLWLTGDE
EECCCEECCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVTVEGKTLPLNEALQWHFELTVNTANIVENYATLTRSETLLPLVGDKAKLQHYAATTLI
CEEECCCCCCCCCCEEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
VDMVRFSPAQLDAEALINLLRPLTPRLYSIASSQAEVENEVHVTVGVVRYDVEGRARAGG
HHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCHHHCCCCEEEEEEEEEEECCCCCCCCC
ASSFLADRVEEEGEVRVFIEHNDNFRLPANLETPVIMIGPGTGIAPFRAFMQQRAADEAP
HHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQKEKVYVQDKLREQGAELW
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
RWINDGAHIYVCGDANRMAKDVEQALLEVIAEFGGMDTEAADEFLSELRVERRYQRDVY
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA