Click here to switch to the map view.

The map label for this gene is aroF

Identifier: 30064001

GI number: 30064001

Start: 2733365

End: 2734435

Strand: Reverse

Name: aroF

Synonym: S2838

Alternate gene names: 30064001

Gene position: 2734435-2733365 (Counterclockwise)

Preceding gene: 30064005

Following gene: 30064000

Centisome position: 59.45

GC content: 51.26

Gene sequence:

>1071_bases
ATGCATAAAGACGCGCTGAATAACGTACATATTACCGACGAACAGGTTTTAATGACTCCGGAACAACTGAAGGCCGCTTT
TCCATTGAGCCTGCAACAAGAAGCCCAGATTGCTGACTCGCGTAAAACCATTTCAGATATTATCGCCGGGCGCGATCCTC
GTCTGCTGGTAGTATGTGGTCCTTGTTCCATTCATGATCCGGAAACTGCTCTGGAATATGCTCGTCGATTTAAAGCTCTT
GCCGCAGAGGTCAGCGATAGCCTCTATCTGGTAATGCGCGTCTATTTTGAAAAACCCCGTACCACTGTCGGCTGGAAAGG
GTTAATTAACGATCCCCATATGGATGGCTCTTTTGATGTAGAAGCCGGGCTGCAAATCGCGCGTAAATTGTTGCTTGAGC
TGGTGAATATGGGACTGCCACTGGCGACGGAAGCGTTAGATCCGAATAGCCCGCAATACCTGGGCGATCTGTTTAGCTGG
TCAGCAATTGGTGCTCGTACAACGGAATCGCAAACTCACCGTGAAATGGCCTCCGGGCTTTCCATGCCGGTTGGTTTTAA
AAACGGCACCGACGGCAGTCTGGCAACAGCAATTAACGCTATGCGCGCCGCCGCCCAGCCGCACTGTTTTGTTGGCATTA
ACCAGGCAGGGCAGGTTGCGTTGCTACAAACTCAGGGGAATCCGGACGGGCATGTGATCCTGCGCGGTGGTAAAGCGCCG
AACTATAGCCCTGCGGATGTTGCGCAATGTGAAAAAGAGATGGAACAGGCGGGACTGCGCCCGTCTCTGATGGTAGATTG
CAGCCACGGTAATTCCAATAAAGATTATCGCCGTCAGCCTGCGGTGGCAGAATCCGTGGTTGCTCAAATCAAAGATGGTA
ATCGCTCAATTATTGGTCTGATGATCGAAAGTAATATCCACGAGGGCAATCAGTCTTCTGAGCAACCGCGCAGTGAAATG
AAATACGGTGTATCCGTAACCGATGCCTGCATTAGCTGGGAAATGACCGATGCCTTGCTGCGTGAAATTCATCAGGATCT
GAACGGGCAGCTGACGGCTCGCGTGGCTTAA

Upstream 100 bases:

>100_bases
TGTACGAAATATGGATTGAAAACTTTACTTTATGTGTTATCGTTACGTCATCCTCGCTGAGGAGCAACTATCGCAAACGA
GCATAAACAGGATCGCCATC

Downstream 100 bases:

>100_bases
GAGGTTTATTATGGTTGCTGAATTGACCGCATTACGCGATCAAATTGATGAAGTCGATAAAGCGCTGCTGAATTTATTAG
CGAAGCGTCTGGAACTGGTT

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQVALLQTQGNPDGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA

Sequences:

>Translated_356_residues
MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQVALLQTQGNPDGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA
>Mature_356_residues
MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKAL
AAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQVALLQTQGNPDGHVILRGGKAP
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEM
KYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=356, Percent_Identity=99.1573033707865, Blast_Score=733, Evalue=0.0,
Organism=Escherichia coli, GI1786969, Length=337, Percent_Identity=55.4896142433234, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1787996, Length=329, Percent_Identity=49.5440729483283, Blast_Score=336, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6320240, Length=331, Percent_Identity=51.6616314199396, Blast_Score=357, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319726, Length=345, Percent_Identity=50.4347826086956, Blast_Score=347, Evalue=1e-96,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 38774; Mature: 38774

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCG
CCCCCCCCCEECCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
EAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQVALLQTQGNPDGHVILRGGKAP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCC
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEE
MIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA
EEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTISDIIAGRDPRLLVVCG
CCCCCCCCCEECCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTTVGWKGLINDPHMDGSFDV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCH
EAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSWSAIGARTTESQTHREMASGL
HHHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQVALLQTQGNPDGHVILRGGKAP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCC
NYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDYRRQPAVAESVVAQIKDGNRSIIGL
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCEEEEE
MIESNIHEGNQSSEQPRSEMKYGVSVTDACISWEMTDALLREIHQDLNGQLTARVA
EEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]