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The map label for this gene is pinE [H]

Identifier: 30063949

GI number: 30063949

Start: 2678519

End: 2678959

Strand: Reverse

Name: pinE [H]

Synonym: S2781

Alternate gene names: 30063949

Gene position: 2678959-2678519 (Counterclockwise)

Preceding gene: 30063951

Following gene: 161486454

Centisome position: 58.25

GC content: 49.89

Gene sequence:

>441_bases
ATGCTTATTGGCTATGTACGCGTATCAACAAATGACCAGAATACCGATCTACAACGTAATGCGTTGAACTGTGCAGGATG
CGAGCTGATTTTTGAAGACAAGATAAGTGGTACAAAGTCCGAAAGACCGGGGCTGAAAAAACTGCTCAAGACATTATCGG
CAGGCGACACACTGGTTGTCTGGAAGCTGGATCGGCTGGGGCGCAGTATGCGGCATCTTGTCGTGCTGGTGGAGGCGTTG
CGCGAACGAGGCATCAACTTTCGTAGTCTGACTGATTCAATTGATACCAGCACACCAATGGGACGCTTTTTCTTCCATGT
GATGGGTGCCCTGGCTGAAATGGAACGTGAACTGATTGTTGAACGAACAAAAGCAGGACTGGAAGCTGCTCGCGCACAGG
GACGAATTGGCGGTCGCCGTCCCAAACTTTCACCAGAATAA

Upstream 100 bases:

>100_bases
ATTTTATAATGACTGTGTATTCCCATGGAAGGAGATCACCCAATTCTCCCCCCCCCCCCCCGACTTTTGACGCAGTGTCT
TGTTAACAGGGGGATTTACC

Downstream 100 bases:

>100_bases
TGGGCGCAGGCCGGGCGACTAATTGCATCAGGAGTTTCTCGCCAGAAGGTGGCGATCATCTATGATGTTGGCGAATCGAC
TTTGTATAAGAGGTTTCCAG

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLKTLSAGDTLVVWKLDRLGRSMRHLVVLVEAL
RERGINFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLSPE

Sequences:

>Translated_146_residues
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLKTLSAGDTLVVWKLDRLGRSMRHLVVLVEAL
RERGINFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLSPE
>Mature_146_residues
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLKTLSAGDTLVVWKLDRLGRSMRHLVVLVEAL
RERGINFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLSPE

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=146, Percent_Identity=97.2602739726027, Blast_Score=291, Evalue=1e-80,
Organism=Escherichia coli, GI1787827, Length=152, Percent_Identity=42.1052631578947, Blast_Score=106, Evalue=6e-25,
Organism=Escherichia coli, GI1787638, Length=152, Percent_Identity=42.1052631578947, Blast_Score=106, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 16314; Mature: 16314

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLKTLSAGDTLVV
CEEEEEEEECCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVVLVEALRERGINFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTKAGLEAARAQGRIGGRRPKLSPE
HHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPGLKKLLKTLSAGDTLVV
CEEEEEEEECCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEE
WKLDRLGRSMRHLVVLVEALRERGINFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIV
EEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTKAGLEAARAQGRIGGRRPKLSPE
HHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]