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The map label for this gene is napF

Identifier: 30063648

GI number: 30063648

Start: 2304461

End: 2304955

Strand: Reverse

Name: napF

Synonym: S2422

Alternate gene names: 30063648

Gene position: 2304955-2304461 (Counterclockwise)

Preceding gene: 30063650

Following gene: 30063647

Centisome position: 50.11

GC content: 54.75

Gene sequence:

>495_bases
GTGAAGATTGATGCATCCCGTCGGGGCATACTCACTGGTTGCTGGCGCAAAGCCAGTAACGGTATCCGTCCGCCCTGGTC
CGGTGATGAATCTCATTTTCTGACCCATTGCACCCGCTGCGACGCCTGTATTAATGCTTGTGAAAACAACATTCTGCAAC
GCGGCACGGGTGGCTATCCGAGCGTTAATTTCAAAAATAACGAGTGCAGCTTCTGTTACGCCTGCGCGCAGGCTTGCCCC
GAATCGCTATTTTCTCCGCGCCACACCAGGGCCTGGGATTTGCAGTTCACCATCGGGGACGCGTGCCTGGCGTATCAGTC
AGTTGAATGCCGCCGCTGTCAGGATAGCTGTGAGCCAATGGCAATTATCTTTCGCCCGACGCTCTCCGGGATCTACCAGG
CGCAACTTAATAGCCAACTTTGCAACGGATGTGGAGCATGTGCAGCCAGCTGTCCGGTATCAGCCATAACTGCGGAGTAT
CTCCATGCACACTAA

Upstream 100 bases:

>100_bases
CATCCTTTAATATCTTAGCGGCTATAAAAATGGCTTATTAATTATGCGGTCTATTTGGTCGCTCTCAATTTTCAGAGCGC
GTTAATGATGGAAGGTCAAT

Downstream 100 bases:

>100_bases
CTGGCAAGTTTGCAGCCTGGTCGTGCAGGCCAAAAGCGAACGAATTTCAGACATCAGCACCCAACTGAACGCCTTTCCCG
GCTGTGAAGTTGCTGTCAGC

Product: ferredoxin-type protein

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGTGGYPSVNFKNNECSFCYACAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
LHAH

Sequences:

>Translated_164_residues
MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGTGGYPSVNFKNNECSFCYACAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
LHAH
>Mature_164_residues
MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGTGGYPSVNFKNNECSFCYACAQACP
ESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
LHAH

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=164, Percent_Identity=98.1707317073171, Blast_Score=333, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 17998; Mature: 17998

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
11.6 %Cys+Met (Translated Protein)
10.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
11.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGTGGYP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
AIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEYLHAH
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACINACENNILQRGTGGYP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDACLAYQSVECRRCQDSCEPM
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCE
AIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEYLHAH
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]