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The map label for this gene is gatC

Identifier: 30063533

GI number: 30063533

Start: 2161463

End: 2162818

Strand: Reverse

Name: gatC

Synonym: S2280

Alternate gene names: 30063533

Gene position: 2162818-2161463 (Counterclockwise)

Preceding gene: 30063534

Following gene: 30063532

Centisome position: 47.02

GC content: 51.25

Gene sequence:

>1356_bases
ATGTTTTCAGAAGTCATGCGTTATATTCTCGACCTCGGCCCTACAGTGATGCTGCCGGTTGTCATCATTATTTTTTCTAA
AATATTAGGCATGAAGGCAGGCGATTGCTTTAAAGCGGGTCTGCATATCGGGATTGGCTTTGTTGGCATTGGCCTTGTGA
TTGGCTTAATGCTGGATTCCATTGGTCCGGCGGCGAAAGCGATGGCGGAAAATTTCGACCTGAATCTGCATGTGGTCGAT
GTCGGCTGGCCGGGCTCTTCACCAATGACCTGGGCGTCGCAAATTGCGCTGGTGGCGATTCCGATTGCGATATTGGTTAA
CGTGGCGATGTTACTGACCCGTATGACGCGGGTGGTAAATGTTGATATCTGGAATATCTGGCATATGACCTTCACCGGCG
CGTTGCTGCACCTGGCAACCGGTTCATGGATGATAGGGATGGCGGGTGTGGTAATTCACGCGGCGTTTGTTTATAAGCTC
GGCGACTGGTTTGCCCGCGATACCCGAAATTTCTTTGAGCTGGAAGGTATTGCTATTCCGCACGGTACGTCGGCGTATAT
GGGGCCGATTGCGGTGCTGGTCGATGCTATCATCGAGAAAATCCCAGGCGTTAACCGAATTAAATTTAGCGCCGACGATA
TTCAGCGCAAATTTGGCCCTTTTGGCGAACCTGTCACCGTGGGTTTTGTGATGGGGCTGATTATCGGCATCCTCGCGGGT
TACGATGTCAAAGGTGTATTGCAGCTGGCGGTAAAAACGGCGGCAGTTATGCTGCTGATGCCACGGATGATTAAACCCAT
CATGGATGGTTTAACGCCCATCGCTAAGCAGGCCCGTAGTCGTTTACAGGCGAAGTTCGGCGGTCAGGAGTTCCTGATTG
GCCTGGATCCCGCATTACTGCTGGGGCATACGGCGGTGGTATCGGCAAGCCTGATTTTTATTCCGCTCACCATTTTAATT
GCTGTTTGTGTTCCGGGTAATCAGGTACTGCCGTTTGGCGATCTTGCCACTATCGGCTTCTTCGTGGCGATGGCGGTCGC
GGTGCATCGTGGAAATCTGTTCCGCACCTTAATCTCGGGTGTCATCATTATGAGCATCACCCTGTGGATCGCGACGCAAA
CTATTGGTTTGCACACCCAACCGGCGGCTAATGCTGGGGCGTTAAAAGCCGGGGGTATGGTGGCTTCAATGGATCAGGGC
GGTTCTCCCATTACCTGGTTACTGATTCAGGTTTTCTCCCCGCAAAATATTCCCGGTTTCATTATTATCGGCGCAATTTA
TCTGACCGGTATTTTCATGACCTGGCGTAGAGCGCGTGGTTTTACTAAACAAGAGAAAGCCGTTCTCGCAGAATAA

Upstream 100 bases:

>100_bases
TTTTGGCGATATTCCGTTAGTTCACGGCATGCCTTTTGTTTCTGGTGTCGGTATCGAAGCATTACAAAATAAAATTCTGA
CTATCTTACAGGGATGACCT

Downstream 100 bases:

>100_bases
TTTTTACCTGAGGGGGAGGTTATCCCCCTTAACAATCAGGGTAATGCTGCCAACTTACTGATTTAGTGTATGATGGTGAT
TTTAAGGTGCTTGCGTGGCT

Product: PTS system galactitol-specific enzyme IIC

Products: galactitol-1-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gat; PTS system galactitol-specific EIIC component [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVD
VGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
YDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILI
AVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGGMVASMDQG
GSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLAE

Sequences:

>Translated_451_residues
MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVD
VGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
YDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILI
AVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGGMVASMDQG
GSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLAE
>Mature_451_residues
MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVD
VGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
YDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILI
AVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGGMVASMDQG
GSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLAE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3775

COG function: function code G; Phosphotransferase system, galactitol-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788408, Length=451, Percent_Identity=98.8913525498891, Blast_Score=885, Evalue=0.0,
Organism=Escherichia coli, GI1790758, Length=384, Percent_Identity=45.5729166666667, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013853
- InterPro:   IPR013014
- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: NA

Molecular weight: Translated: 48328; Mature: 48328

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDS
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
IGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVN
CCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGIAIP
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECC
HGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
YDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHH
LGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIIMSITLWIATQTIGLHTQPAANAGALKAGGMVASMDQGGSPITWLLIQVFSPQNIPGF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHEECCCCCCCCHH
IIIGAIYLTGIFMTWRRARGFTKQEKAVLAE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIGIGFVGIGLVIGLMLDS
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
IGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPIAILVNVAMLLTRMTRVVN
CCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKLGDWFARDTRNFFELEGIAIP
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEECC
HGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKFGPFGEPVTVGFVMGLIIGILAG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
YDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAKQARSRLQAKFGGQEFLIGLDPALL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCHHHH
LGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGDLATIGFFVAMAVAVHRGNLFRTLISG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
VIIMSITLWIATQTIGLHTQPAANAGALKAGGMVASMDQGGSPITWLLIQVFSPQNIPGF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHEECCCCCCCCHH
IIIGAIYLTGIFMTWRRARGFTKQEKAVLAE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; galactitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + galactitol [Periplasm] = galactitol-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]